FastQCFastQC Report
Thu 26 May 2016
SRR1926465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942989
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA36320.38515825741339504No Hit
GTCCTACAGTGGACATTTCTAAATT33930.35981331701642333No Hit
CTTTAGGACGTGAAATATGGCGAGG26790.28409663315266664No Hit
GTCCTAAAGTGTGTATTTCTCATTT25740.2729618267021142No Hit
GTCCTACAGTGTGCATTTCTCATTT23670.251010351128168No Hit
CTGTAGGACCTGGAATATGGCGAGA19900.2110310936818987No Hit
CTGAAGGACCTGGAATATGGCGAGA14740.1563114734106124No Hit
CCCATGTACTCTGCGTTGATACCAC10850.1150596666557086No Hit
GTACATGGGAAGCAGTGGTATCAAC9570.10148580736360657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA651.8267747E-716.14072
TAGGACC7100.014.5041584
ATTCCCG400.00534383214.22301611
TAGCGAG400.005370225314.21245718
CCGGTTT759.869764E-713.90694812
GTCTAGG551.9018856E-413.8663341
AAGGCGT706.983695E-613.621636
AAAGGCG957.026938E-813.0480875
GTAGGAC19450.012.8483663
TCCAACG1550.012.83705718
TGTAGGA19850.012.732932
GTCCTAT751.4252022E-512.7108081
GGCGTGC604.0869424E-412.6675538
GGCGAGG5800.012.5788419
CTGTAGG19650.012.5652651
TCCTATA1155.007678E-912.4404592
AGGACCT13850.012.3233945
AGGCGTG853.9378992E-612.2943247
CGGTTTC854.0230007E-612.27083713
TATAGCG550.003128098612.05905316
Produced by FastQC (version 0.11.5)