FastQCFastQC Report
Thu 26 May 2016
SRR1926464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999083
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18190.18206695539810006No Hit
CTGTAGGACGTGGAATATGGCAAGA12940.1295187687109079No Hit
GTCCTAAAGTGTGTATTTCTCATTT12400.12411381236593956No Hit
GTCCTACAGTGTGCATTTCTCATTT11400.11410463394933154No Hit
CTTTAGGACGTGAAATATGGCGAGG10030.10039205951857853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG400.002321806716.1200984
TGTAGGA10650.015.2141272
TAGGACC4300.014.9954414
GTCCTAA5850.014.18347551
CTGTAGG10450.013.9590881
GTCCTAC12050.013.7715071
ATTTAAG700.001176999213.7313952
TGTTGGG802.063925E-413.7313942
TAGGATA808.055918E-613.4334144
CTAGGAC901.5044188E-613.3946443
GATTTAC600.007992567513.3827961
TCCTACA13150.013.3659192
GTAGGAC11900.013.3537123
TCCTAAA6350.013.1907872
TGTAGAA2400.013.1592532
ACTGTCG450.00961081313.0152478
TAGAGGT756.554215E-512.8960784
GTAGAAC2300.012.86521153
TATTAAC750.001979340312.8159682
TTAGTAC600.002015618112.7857973
Produced by FastQC (version 0.11.5)