FastQCFastQC Report
Thu 26 May 2016
SRR1926464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999083
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA42230.42268760453335713No Hit
GTCCTACAGTGGACATTTCTAAATT39610.39646355708184405No Hit
CTTTAGGACGTGAAATATGGCGAGG30300.3032781060232233No Hit
GTCCTAAAGTGTGTATTTCTCATTT29000.29026617408163286No Hit
GTCCTACAGTGTGCATTTCTCATTT26760.26784561442843086No Hit
CTGTAGGACCTGGAATATGGCGAGA22070.2209025676545392No Hit
CTGAAGGACCTGGAATATGGCGAGA17850.17866383473645334No Hit
CCCATGTACTCTGCGTTGATACCAC14340.14353161849415913No Hit
GTACATGGGAAGCAGTGGTATCAAC12990.1300192276317383No Hit
GAATATGGCAAGAAAACTGAAAATC11450.11460509287016193No Hit
GTCCTTCAGTGTGCATTTCTCATTT10640.10649765835270943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG250.006097432718.96377610
TAGGACC7700.014.2469184
TTACACC1051.2860255E-913.6274874
TTAATCC1206.91216E-1012.7189884
AGGACCT12900.012.5710935
GTCCTAT1750.011.990981
CTTATAC1052.564302E-711.8140613
GTCCTAC20400.011.782581
GTAGGAC19800.011.7107923
GGACCTG12850.011.6543616
TGTAGGA20600.011.6277452
CTGTAGG19950.011.6180541
CCTACAC1900.011.5509963
GGCGAGG8500.011.47716619
TCCAACG752.1291655E-411.36687518
TCCTACA22450.011.1371462
CCAACGA600.00600621611.05002219
GCTTTAA951.2994757E-511.0443241
ACGAAAT951.3900868E-510.97737812
TCTAAAT12800.010.95245818
Produced by FastQC (version 0.11.5)