FastQCFastQC Report
Thu 26 May 2016
SRR1926463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620577
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG1051.6007107E-1017.8041781
TGCTAGA551.9168478E-417.432312
GTCTTAT654.4546432E-516.4346261
TGTAGGA2050.016.0352952
GTCCTAT708.3931845E-515.2607231
ATTATAC450.004507198514.58999353
GTAGGAC2050.014.4120663
TAGGACA901.2437704E-714.2846534
CTAGGCT450.005186879514.2846534
GTCCTAC2450.014.1706721
AGGGTAT400.00652919913.79780410
TAACACA550.001240341813.635354
TCTACAC1054.274625E-813.5478523
CCCCTAC600.00810272913.3531331
GTCCAGT700.001451273913.3531331
GGCGAGG804.872363E-712.77135619
ATAATCC600.002036775212.7662443
GGACGTG2150.012.5976346
TCCTACA3200.012.412742
CAGGAGT801.2313755E-412.0526764
Produced by FastQC (version 0.11.5)