##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926463_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 620577 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.92257366934321 27.0 7.0 30.0 2.0 31.0 2 16.3175077387657 16.0 2.0 30.0 2.0 31.0 3 20.049787536437865 24.0 15.0 31.0 2.0 31.0 4 22.33871864410057 28.0 2.0 35.0 2.0 37.0 5 22.654324926640854 29.0 2.0 35.0 2.0 37.0 6 23.550115457066568 31.0 2.0 35.0 2.0 37.0 7 23.480261111836242 31.0 7.0 35.0 2.0 37.0 8 23.62961888693909 31.0 8.0 35.0 2.0 37.0 9 26.286512390887836 32.0 17.0 37.0 2.0 39.0 10 26.966921107292084 32.0 23.0 37.0 2.0 39.0 11 24.075769807775668 30.0 7.0 37.0 2.0 39.0 12 25.179278316792274 31.0 11.0 37.0 2.0 39.0 13 21.83718217078622 27.0 2.0 35.0 2.0 39.0 14 25.201186959877663 31.0 17.0 38.0 2.0 40.0 15 26.269782798911336 31.0 17.0 38.0 2.0 40.0 16 26.658050491719802 31.0 17.0 39.0 2.0 40.0 17 25.948209488911125 31.0 11.0 38.0 2.0 40.0 18 26.014056273435852 31.0 16.0 38.0 2.0 40.0 19 26.424458205831026 31.0 18.0 39.0 2.0 40.0 20 26.65853713560122 31.0 18.0 39.0 2.0 40.0 21 24.494520422123284 30.0 2.0 37.0 2.0 40.0 22 25.435763813354345 31.0 2.0 38.0 2.0 40.0 23 25.434084730823088 31.0 2.0 38.0 2.0 40.0 24 24.500534180287055 31.0 2.0 38.0 2.0 40.0 25 24.292891937664464 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.471775248978023 1101 2 10.378829699430753 1101 3 7.140927313660903 1101 4 8.723654642898474 1101 5 8.263109867542749 1101 6 7.922368495857334 1101 7 7.587779903672821 1101 8 6.147608356911231 1101 9 5.842508362457298 1101 10 5.459564849408011 1101 11 8.50047615799052 1101 12 5.837205373057454 1101 13 10.574510424391104 1101 14 8.137150145634514 1101 15 7.199723840921575 1101 16 6.730273526695235 1101 17 3.1290961022056223 1101 18 5.31914266172204 1101 19 5.633856953273575 1101 20 3.656113680275702 1101 21 0.2102331661551453 1101 22 4.547448457455964 1101 23 5.579437943959707 1101 24 6.887314342405844 1101 25 7.392126366639516 1102 1 5.461891470905389 1102 2 10.554901005146021 1102 3 6.918207523990649 1102 4 8.136813766196916 1102 5 7.878748228150151 1102 6 6.886420783439032 1102 7 7.0596642714550235 1102 8 7.1695627527046355 1102 9 6.07600263736024 1102 10 5.437647310462609 1102 11 8.072881435891155 1102 12 5.610345330794935 1102 13 10.224136941664678 1102 14 7.344715627967801 1102 15 5.082961346450102 1102 16 5.6129704651197265 1102 17 6.408452581639498 1102 18 6.603278245780224 1102 19 5.580764663726175 1102 20 5.754245038081983 1102 21 4.687848773801715 1102 22 5.63302391570944 1102 23 5.990582226234018 1102 24 7.573607280633766 1102 25 7.216730769804293 1103 1 4.606916746559115 1103 2 9.119431133583262 1103 3 6.369082409504717 1103 4 7.926840355332644 1103 5 7.215419403255051 1103 6 6.897684779122294 1103 7 6.916664243055411 1103 8 6.367939359945119 1103 9 5.412487440018445 1103 10 4.541199391799093 1103 11 5.661068440229194 1103 12 4.349204695921632 1103 13 8.67547243336844 1103 14 6.777403225147715 1103 15 5.69603794730067 1103 16 5.377686788506097 1103 17 3.005640487665186 1103 18 1.8638402385809236 1103 19 3.9282205910799703 1103 20 4.5796184929656505 1103 21 6.935202285058608 1103 22 2.083753253217516 1103 23 4.644255183414504 1103 24 6.241096648529425 1103 25 1.7220264097540259 1104 1 4.873044987170754 1104 2 9.46432872578156 1104 3 6.573780737152347 1104 4 7.25794023692756 1104 5 7.1327869011896965 1104 6 6.550568503244527 1104 7 6.486536707835764 1104 8 6.373820522243481 1104 9 5.420112970691267 1104 10 4.252326602990156 1104 11 7.265803079927647 1104 12 4.686997114347651 1104 13 8.584729660813057 1104 14 6.078851450504821 1104 15 5.4044504417735055 1104 16 4.929769823220592 1104 17 5.343741297910398 1104 18 3.425447802606975 1104 19 3.9610803909467123 1104 20 2.9140203387992756 1104 21 1.7622205357733414 1104 22 4.063306441250521 1104 23 4.4155109809635285 1104 24 5.513618589955829 1104 25 4.703853878864891 1105 1 3.474370204235065 1105 2 9.877200824036315 1105 3 6.873636699118247 1105 4 6.06502160330794 1105 5 2.6688736329029084 1105 6 5.573334234951023 1105 7 6.417143279193347 1105 8 3.073835295375183 1105 9 4.452134573038855 1105 10 4.066447870256251 1105 11 7.148158588627009 1105 12 4.5197493152085215 1105 13 5.498250601727243 1105 14 4.277759917523806 1105 15 4.680417578270529 1105 16 5.05225755144869 1105 17 5.794127078099628 1105 18 3.0877810173075844 1105 19 2.9193437757397867 1105 20 2.0211691915312997 1105 21 2.9969152355765907 1105 22 4.563440742447817 1105 23 1.634255734823011 1105 24 5.037680328216226 1105 25 5.928690870786674 1106 1 5.980949108223619 1106 2 10.91732043447816 1106 3 7.7101696447396755 1106 4 8.874945511549456 1106 5 8.446951425846287 1106 6 7.487337030696743 1106 7 7.614098290105986 1106 8 7.043731575846504 1106 9 6.129930648444862 1106 10 5.467376103991146 1106 11 7.74780977978612 1106 12 4.622551489823941 1106 13 3.7147486334958835 1106 14 5.375556619335104 1106 15 5.719335479614994 1106 16 5.911997164659908 1106 17 6.15278037266177 1106 18 6.333140672850611 1106 19 6.147684164374816 1106 20 5.596515857406718 1106 21 5.817977618949815 1106 22 6.07514463077624 1106 23 6.349153960447602 1106 24 7.2848153482282285 1106 25 6.327002213997432 1107 1 3.7862955265810996 1107 2 -13.812580197861497 1107 3 -5.710546789656409 1107 4 1.373293999693189 1107 5 4.375760879254919 1107 6 4.492951763730158 1107 7 4.691705092624268 1107 8 4.977743597060794 1107 9 3.2815602080226967 1107 10 2.4818719387601327 1107 11 5.035000156949888 1107 12 2.6950445111172456 1107 13 4.271315867337744 1107 14 3.4295258274972795 1107 15 3.322606898563027 1107 16 3.316747491093139 1107 17 3.9613850141635503 1107 18 -0.967881676116491 1107 19 1.6000591239524873 1107 20 1.8726991422332517 1107 21 -2.232373332410617 1107 22 1.4094880132843066 1107 23 -1.4775484815091886 1107 24 1.6494944173212929 1107 25 2.7163044899477526 1108 1 5.545538087446744 1108 2 10.875376006518067 1108 3 0.5971768451036503 1108 4 6.300423063161954 1108 5 7.669786041662032 1108 6 7.742129855388981 1108 7 7.702727220799186 1108 8 7.208871791725546 1108 9 5.939360850411116 1108 10 4.714258089234356 1108 11 7.420056398845986 1108 12 2.295296760463998 1108 13 3.323068119975421 1108 14 5.090716121903366 1108 15 5.2360745125770976 1108 16 5.342403536850149 1108 17 0.2548935014572926 1108 18 3.7447153238769246 1108 19 4.962226987418617 1108 20 5.257483060144438 1108 21 5.519725454554692 1108 22 2.124572129309133 1108 23 4.970022158180676 1108 24 5.589687627486299 1108 25 6.24438430144361 1109 1 3.0913324799701094 1109 2 -13.812580197861497 1109 3 -5.797653975552603 1109 4 1.6568457386514162 1109 5 0.9998307241096747 1109 6 1.982949488378953 1109 7 3.1395936509786004 1109 8 3.35620107748856 1109 9 2.80011021939945 1109 10 1.7726062030211054 1109 11 -0.10886440066284919 1109 12 0.49750189041829174 1109 13 2.086419985723424 1109 14 2.7566227321769183 1109 15 2.6052999031221553 1109 16 2.537641956933374 1109 17 2.9614372629689925 1109 18 3.125593964984777 1109 19 1.6531506520615018 1109 20 0.5896800040014547 1109 21 -0.46517631227797196 1109 22 1.75172780159237 1109 23 2.2921826056873265 1109 24 3.4685180388376864 1109 25 3.9455815181705347 1110 1 3.2276942212757405 1110 2 8.885375192473077 1110 3 6.041865879917641 1110 4 6.931301146357352 1110 5 7.784060998924669 1110 6 4.029145159434783 1110 7 6.115471773481467 1110 8 6.685739346605747 1110 9 4.738261211304394 1110 10 4.359405702725795 1110 11 2.5004501252726 1110 12 5.095024733763321 1110 13 9.60358463152129 1110 14 6.860962099164375 1110 15 6.333709033511621 1110 16 5.823748339172891 1110 17 6.1829760184844 1110 18 6.10026304048073 1110 19 4.848335309671896 1110 20 5.105388929408811 1110 21 3.094471743866279 1110 22 4.874684060495195 1110 23 5.475177776447843 1110 24 6.611812691931526 1110 25 7.237865157818938 1111 1 4.935291060914263 1111 2 9.029139838735027 1111 3 7.033153798643429 1111 4 7.9136652613575045 1111 5 8.169414566915638 1111 6 7.159260935053201 1111 7 7.201050158404787 1111 8 7.5078346676246 1111 9 5.892876420629026 1111 10 5.050604681016164 1111 11 7.156356076289036 1111 12 6.395869582870684 1111 13 7.9886448491277555 1111 14 5.996816488536421 1111 15 5.5270243860252535 1111 16 5.227571927604853 1111 17 6.509415476066785 1111 18 6.373247255679171 1111 19 4.950257987612286 1111 20 4.766679773135536 1111 21 7.465621201210585 1111 22 6.922804577336059 1111 23 6.698368102177309 1111 24 6.713906278356802 1111 25 7.02768513421486 1112 1 -1.8619385480672754 1112 2 7.518260923633832 1112 3 5.4967385478118835 1112 4 -0.46558416789063983 1112 5 3.721326051212479 1112 6 2.954912105201384 1112 7 5.445201127614112 1112 8 5.7272291148717365 1112 9 4.277680312857395 1112 10 2.9146622777874605 1112 11 -0.6453129987936883 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4.359692672839955 2204 7 5.430941254234554 2204 8 5.312844343779098 2204 9 3.643544475827376 2204 10 3.6214455156530043 2204 11 4.808237972393702 2204 12 4.89504700433362 2204 13 3.008238324153158 2204 14 4.328210655860108 2204 15 4.497853415331985 2204 16 4.2939794391677495 2204 17 -0.6243330113752954 2204 18 3.017169829578428 2204 19 3.729691416090958 2204 20 4.156737155472232 2204 21 5.721416564245565 2204 22 5.175923503388315 2204 23 3.7979494345774576 2204 24 5.282308469088775 2204 25 5.452257085593764 2205 1 6.164113380503551 2205 2 10.705904923869792 2205 3 7.5031592518223995 2205 4 8.0928611773307 2205 5 8.373055739489363 2205 6 8.005124892675152 2205 7 7.964022495690092 2205 8 7.86953800643861 2205 9 6.602570126868503 2205 10 6.041835870229928 2205 11 8.221654800773152 2205 12 7.218939912379643 2205 13 10.264102161296265 2205 14 7.767668298191602 2205 15 6.53090266051073 2205 16 5.921797085322176 2205 17 6.848670857265443 2205 18 6.720125537589553 2205 19 5.563088880489907 2205 20 5.601283452422614 2205 21 -0.8689828905711217 2205 22 4.107414072797379 2205 23 5.556716622187835 2205 24 7.618836166645007 2205 25 7.88672829517769 2206 1 4.227520069428724 2206 2 9.077509892228594 2206 3 6.261645510849686 2206 4 2.292965971033329 2206 5 6.148369917508681 2206 6 6.424129921148172 2206 7 6.6425657819881145 2206 8 6.629973915747378 2206 9 5.127440353271641 2206 10 4.478289456367911 2206 11 4.176855899076141 2206 12 5.2262381032666525 2206 13 8.511459978987716 2206 14 0.9915726353511225 2206 15 2.9801841331465724 2206 16 4.034341460174932 2206 17 5.0961261574709695 2206 18 -0.6666347122906267 2206 19 1.5796893402824317 2206 20 3.1095166630904494 2206 21 4.292068783284883 2206 22 -1.2521704892218075 2206 23 -1.6600528072957523 2206 24 2.400260061233915 2206 25 3.923943057279736 2207 1 1.391973187995191 2207 2 -13.812580197861497 2207 3 -6.218775129168758 2207 4 -4.387709951690127 2207 5 1.05863063526483 2207 6 1.2423883990044544 2207 7 2.7759551128180284 2207 8 2.608790392187256 2207 9 0.02479200108032842 2207 10 -0.042215509292446995 2207 11 -1.8734990262330804 2207 12 -3.046612992801993 2207 13 -0.16597797084511612 2207 14 -6.061405937371511 2207 15 -2.000666370007405 2207 16 -1.057968765956133 2207 17 0.2096116722361927 2207 18 0.19229935889207184 2207 19 -0.3870003841769467 2207 20 -0.2936735472295595 2207 21 -3.9370910895678612 2207 22 -4.55795799519213 2207 23 -1.6377888614486231 2207 24 0.08985276179553736 2207 25 0.5744560931675444 2208 1 4.836849842131581 2208 2 6.258970057675132 2208 3 6.393945954393743 2208 4 8.11633721280321 2208 5 3.443684311971289 2208 6 6.105817230263078 2208 7 6.670068582915103 2208 8 6.880995294877788 2208 9 5.133564023790541 2208 10 4.693561798556779 2208 11 6.885844956276443 2208 12 6.2986370780506356 2208 13 9.12003618722321 2208 14 3.6737142318027693 2208 15 5.14560304439172 2208 16 5.438046357576592 2208 17 6.23359411947045 2208 18 0.6598597549193421 2208 19 3.575629914587573 2208 20 4.767404533925507 2208 21 7.26719962110737 2208 22 6.3015996352467845 2208 23 1.8762103724086536 2208 24 3.579427303433526 2208 25 4.869730923510534 2209 1 2.849966131727186 2209 2 4.563803565976141 2209 3 5.08711341417737 2209 4 0.3097356927846029 2209 5 4.739321016208859 2209 6 5.1228467191269615 2209 7 5.905372869518448 2209 8 4.412806933266744 2209 9 4.172543574574124 2209 10 3.342032350553609 2209 11 -0.5189637212822227 2209 12 3.385429951941898 2209 13 2.2694716807263546 2209 14 3.6068330987665647 2209 15 3.951049463421498 2209 16 3.574835459709522 2209 17 1.0203640484985286 2209 18 2.9513035211457606 2209 19 3.763584490044739 2209 20 3.8988188804369486 2209 21 6.113319739037681 2209 22 4.96670521304873 2209 23 0.6520594895028928 2209 24 -3.112264455903148 2209 25 0.2375496528051535 2210 1 -1.7587254784447275 2210 2 -13.812580197861497 2210 3 -7.535426561222385 2210 4 -1.8964379677892147 2210 5 -0.4865399924012266 2210 6 -1.005882649562075 2210 7 -0.7560304887011817 2210 8 -21.046602660829198 2210 9 -12.96008321681007 2210 10 -6.798947082496532 2210 11 -9.665009803836071 2210 12 -4.447510063504769 2210 13 -5.055348108169209 2210 14 -3.8167345090025364 2210 15 -5.6280968222274055 2210 16 -5.047357680826533 2210 17 -4.600945914601102 2210 18 -4.752356480570537 2210 19 -10.335982145156496 2210 20 -7.072837202402951 2210 21 -5.0774001042072605 2210 22 -9.563426512687133 2210 23 -7.662001267383747 2210 24 -10.12854781641117 2210 25 -8.000622205657619 2211 1 -0.24527325175169068 2211 2 4.403451082685912 2211 3 0.16386473887536823 2211 4 0.9413425000550184 2211 5 0.5700338101403162 2211 6 -0.3254909607307752 2211 7 -20.852929713507383 2211 8 -13.426856815276901 2211 9 -8.94592626848208 2211 10 -7.183833076722362 2211 11 -9.485575740774852 2211 12 -4.882414244095372 2211 13 -6.818769077047792 2211 14 -4.7004724889520695 2211 15 -5.015929575870363 2211 16 -11.539088568093804 2211 17 -7.662651681952031 2211 18 -5.631650167628663 2211 19 -8.277262919895634 2211 20 -6.514984671088303 2211 21 -4.178848761826213 2211 22 -5.576904313955634 2211 23 -7.289999175951799 2211 24 -22.000640839666985 2211 25 -21.804885771549092 2212 1 -1.0790953436383006 2212 2 -13.812580197861497 2212 3 -7.8224500216329425 2212 4 -19.790817812750454 2212 5 -20.028206659067894 2212 6 -20.899293502275253 2212 7 -9.984894522891548 2212 8 -9.571529347046207 2212 9 -5.315427734757737 2212 10 -5.576794953555208 2212 11 -5.7475503253300815 2212 12 -2.7524044689243077 2212 13 -6.037732908914849 2212 14 -3.6027207782971296 2212 15 -3.5936421858410377 2212 16 -3.981903817360667 2212 17 -3.9862098442198715 2212 18 -3.109656032731305 2212 19 -3.7601563705310213 2212 20 -3.9138116505604437 2212 21 -1.8572456337714698 2212 22 -3.1702571976310985 2212 23 -3.4209864682294118 2212 24 -2.404355404671872 2212 25 -2.8694019005813516 2213 1 -18.454461912553256 2213 2 -13.812580197861497 2213 3 -8.953299015670401 2213 4 -6.25812756623384 2213 5 -20.028206659067894 2213 6 -11.115799397237739 2213 7 -20.852929713507383 2213 8 -11.036956358578394 2213 9 -8.692871920416838 2213 10 -7.698327675233141 2213 11 -6.994943874974435 2213 12 -5.615759521367629 2213 13 -19.168720201192464 2213 14 -12.683512095682163 2213 15 -7.790242563646682 2213 16 -6.978169764876483 2213 17 -5.570265314386738 2213 18 -5.465852579675698 2213 19 -6.270850910579362 2213 20 -6.301653349365736 2213 21 -4.47032801866176 2213 22 -5.386219329058427 2213 23 -5.014477650145032 2213 24 -4.0703085781450135 2213 25 -4.982806457508364 2214 1 -18.454461912553256 2214 2 -13.812580197861497 2214 3 -12.298269698477286 2214 4 -19.790817812750454 2214 5 -20.028206659067894 2214 6 -20.899293502275253 2214 7 -16.499988537036796 2214 8 -15.03601442553508 2214 9 -13.966772683294337 2214 10 -13.864095281310943 2214 11 -14.598067259376428 2214 12 -12.401244673627888 2214 13 -19.168720201192464 2214 14 -16.774988042974265 2214 15 -23.766662713700274 2214 16 -18.17041741634865 2214 17 -23.475945914601102 2214 18 -17.646104770584216 2214 19 -14.778636043925305 2214 20 -13.548088000396568 2214 21 -10.980448439820577 2214 22 -12.010273297912851 2214 23 -12.01970304576952 2214 24 -10.907699663196397 2214 25 -10.621356359784386 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 111257.0 3 2.0 4 619.0 5 2023.0 6 2759.0 7 2602.0 8 2353.0 9 2197.0 10 2169.0 11 1923.0 12 1859.0 13 2070.0 14 3078.0 15 6183.0 16 9048.0 17 9942.0 18 10178.0 19 11876.0 20 13820.0 21 15919.0 22 16905.0 23 18399.0 24 19298.0 25 19659.0 26 16576.0 27 13346.0 28 14204.0 29 16230.0 30 16042.0 31 18143.0 32 23971.0 33 29079.0 34 35230.0 35 40804.0 36 39942.0 37 41916.0 38 28956.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79103047766159 17.898942490494296 11.963076283269961 26.346950748574145 2 14.984439781447353 20.00172328153352 39.932234487931964 25.08160244908717 3 18.976441001773882 24.009041847569065 29.22927468880235 27.785242461854704 4 11.626120263007731 16.186611668264156 38.17472186895114 34.01254619977697 5 13.36632925911857 37.13773378321007 36.09087658927893 13.405060368392435 6 32.50264139246272 36.958325536736616 17.81253012321458 12.726502947586079 7 28.09190511118728 31.3420085639338 22.402625657346746 18.163460667532178 8 25.34865459114229 36.506474016682375 20.66554985516351 17.47932153701183 9 26.276684636553394 15.263595977584421 19.723805744556607 38.73591364130557 10 13.984992385073436 29.351129819790966 34.933884135460715 21.72999365967488 11 33.86877816464288 22.238691026076683 24.639740172364625 19.252790636915808 12 23.42632356360354 24.696206080665235 31.330094232531426 20.547376123199804 13 28.184327092113648 21.089065966544606 25.47384905921098 25.252757882130766 14 20.499424030997602 21.63205398400377 27.81193957561557 30.056582409383058 15 22.842333114939002 30.382411239291073 23.386217935427155 23.38903771034277 16 22.27318055788316 28.17606953047351 26.894441577577155 22.656308334066182 17 21.711618343268857 28.019500708890565 28.301398711560307 21.967482236280272 18 21.539870178708654 27.491375222772678 29.56377430061641 21.404980297902256 19 22.968989004954963 27.103570448038276 27.202767718233993 22.724672828772764 20 23.235337840458122 27.349274163316483 27.824332010496516 21.591055985728875 21 22.44010954286918 26.162881820096906 26.879966294501788 24.517042342532125 22 22.586119985335706 27.541701581648688 27.82451293600084 22.04766549701477 23 22.087992566734847 27.40677769246156 27.859258351131444 22.645971389672148 24 22.573430981605956 27.109613491327107 27.974246920750495 22.342708606316446 25 22.53692892643574 27.018084970810136 27.811591093502734 22.633395009251387 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 484.0 1 484.0 2 1171.0 3 1858.0 4 1858.0 5 1858.0 6 2690.5 7 3523.0 8 3523.0 9 3523.0 10 4117.5 11 4712.0 12 4712.0 13 4712.0 14 5747.0 15 6782.0 16 6782.0 17 6782.0 18 9592.5 19 12403.0 20 12403.0 21 12403.0 22 17361.5 23 22320.0 24 22320.0 25 22320.0 26 29260.0 27 36200.0 28 36200.0 29 36200.0 30 43805.0 31 51410.0 32 51410.0 33 51410.0 34 57972.0 35 64534.0 36 64534.0 37 64534.0 38 69430.0 39 74326.0 40 74326.0 41 74326.0 42 75863.5 43 77401.0 44 77401.0 45 77401.0 46 76520.0 47 75639.0 48 75639.0 49 75639.0 50 70948.0 51 66257.0 52 66257.0 53 66257.0 54 58062.0 55 49867.0 56 49867.0 57 49867.0 58 41852.5 59 33838.0 60 33838.0 61 33838.0 62 27231.5 63 20625.0 64 20625.0 65 20625.0 66 15646.5 67 10668.0 68 10668.0 69 10668.0 70 7771.0 71 4874.0 72 4874.0 73 4874.0 74 3380.0 75 1886.0 76 1886.0 77 1886.0 78 1289.0 79 692.0 80 692.0 81 692.0 82 445.0 83 198.0 84 198.0 85 198.0 86 128.5 87 59.0 88 59.0 89 59.0 90 38.0 91 17.0 92 17.0 93 17.0 94 9.5 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.205935766230459 2 36.41465926065581 3 5.616708321449232 4 13.587193853462182 5 13.461826654871192 6 14.744342764878493 7 9.268471116396515 8 7.6569063951773915 9 0.10490237311405354 10 1.3891910270603003 11 5.633144637974015 12 5.743203502546823 13 16.037494138519477 14 1.5200369978262165 15 2.850895215259347 16 2.885379251889774 17 2.8235013543847094 18 1.4449455909580922 19 1.396281202816089 20 0.27474431053680687 21 4.383340020658194 22 1.5419520865259266 23 0.10651377669491457 24 8.996305051589086 25 0.1079640399176895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 620577.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.59337018255101 #Duplication Level Percentage of deduplicated Percentage of total 1 97.52589501204137 93.22828984270676 2 1.9459135182075717 3.7203286257849326 3 0.2885375236289739 0.8274682292346319 4 0.09058772644885933 0.34638344273685906 5 0.044063464325203076 0.21060875283823838 6 0.02495456026339519 0.14312943102009082 7 0.015662074289429666 0.10480333257632453 8 0.009361211381466228 0.07158957959564886 9 0.009121979339212606 0.07848006729938395 >10 0.040949076973171857 0.7197616614235698 >50 0.003074831857377616 0.20362967527878714 >100 0.001879021243961684 0.3455273595047939 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAG 105 1.6007107E-10 17.804178 1 TGCTAGA 55 1.9168478E-4 17.43231 2 GTCTTAT 65 4.4546432E-5 16.434626 1 TGTAGGA 205 0.0 16.035295 2 GTCCTAT 70 8.3931845E-5 15.260723 1 ATTATAC 45 0.0045071985 14.5899935 3 GTAGGAC 205 0.0 14.412066 3 TAGGACA 90 1.2437704E-7 14.284653 4 CTAGGCT 45 0.0051868795 14.284653 4 GTCCTAC 245 0.0 14.170672 1 AGGGTAT 40 0.006529199 13.797804 10 TAACACA 55 0.0012403418 13.63535 4 TCTACAC 105 4.274625E-8 13.547852 3 CCCCTAC 60 0.008102729 13.353133 1 GTCCAGT 70 0.0014512739 13.353133 1 GGCGAGG 80 4.872363E-7 12.771356 19 ATAATCC 60 0.0020367752 12.766244 3 GGACGTG 215 0.0 12.597634 6 TCCTACA 320 0.0 12.41274 2 CAGGAGT 80 1.2313755E-4 12.052676 4 >>END_MODULE