FastQCFastQC Report
Thu 26 May 2016
SRR1926463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620577
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8460.13632474294084376No Hit
CTGTAGGACGTGGAATATGGCAAGA6490.10458009239788134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGC704.2405918E-714.9843073
TAGGACC1650.013.8663994
CTATACT1251.3278623E-912.2024324
CCTAGAC550.002980475312.136053
GTACTAA550.002991355512.130151
GTTCAGG550.002991355512.130151
TGTAGGA4500.012.0772812
TGGCGAG3750.011.87793618
GCCATAT1052.5978625E-711.8000771
GGACCTG3250.011.7321576
GTCTTAG657.7965466E-411.7302551
TATACTG1251.7307684E-811.4397795
AGGACCT3350.011.3828665
TCTGAAC600.005708282311.1247133
TCTATTA600.005711729611.1238112
TTAGCCT600.005718629311.1220084
TAAGGTC600.005718629311.1220085
GTCCTAG600.00572899211.1193051
GCTATAA600.00572899211.1193051
GTATAAG600.00572899211.1193051
Produced by FastQC (version 0.11.5)