FastQCFastQC Report
Thu 26 May 2016
SRR1926462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738913
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA13540.18324214082036722No Hit
GCTTACTCTGCGTTGATACCACTGC11260.15238600484766138No Hit
CTGCTTACTCTGCGTTGATACCACT8430.11408650274118874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAG551.0516629E-519.4404051
GTATGGA350.005856856719.0932561
GCCCTAC350.005856856719.0932561
CTTGTAC905.506081E-915.8299383
AATACTT558.761915E-515.1867375
TGTAGGA3500.014.491382
TTAACCC450.00511714214.31452754
GTATAAC751.4996882E-414.2562961
ATACTTA450.006163451413.9185746
CAATACT703.4581375E-513.8032964
GTCCTAC3300.013.77028751
CTTAGTC806.572687E-613.6989843
CCTAGAG652.4007732E-413.4882313
ATTACAT652.4007732E-413.4882313
TTAAAAC652.4007732E-413.4882313
CTAATAC652.4007732E-413.4882313
CTAGCAC652.4007732E-413.4882313
CTCTTAC600.00805745113.3652791
CCTGTAC1159.582436E-913.34161953
AAGCAAT550.001509312113.2859126
Produced by FastQC (version 0.11.5)