FastQCFastQC Report
Thu 26 May 2016
SRR1926462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738913
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA35910.48598414157011716No Hit
GCTTACTCTGCGTTGATACCACTGC26330.35633423691287064No Hit
CTGCTTACTCTGCGTTGATACCACT20180.27310387014438775No Hit
GTACATGGGAAGCAGTGGTATCAAC15540.21030892676133728No Hit
CCCATGTACTCTGCGTTGATACCAC12770.17282142823309377No Hit
GCGTTGATACCACTGCTTACTCTGC10520.14237129404950244No Hit
CTGTAGGACGTGGAATATGGCAAGA8900.12044719743731672No Hit
GTCCTACAGTGGACATTTCTAAATT8380.11340983309266449No Hit
GAGTACATGGGAAGCAGTGGTATCA8240.11151515807679659No Hit
GTATCAACGCAGAGTACTTTTTTTT7460.10095911155981827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG350.00211595316.3458925
GCACCGT350.00211595316.3458926
GTAGGAC5850.012.7143373
TGTAGGA5800.012.6603962
TAGGACC2950.012.605734
CTAGGAC1103.6074198E-812.1364133
GACGTGG2600.012.0644567
CTGTAGG5700.012.0410621
GTAGCCC907.140523E-611.654813
GTCCTAC4600.011.6047921
TTAGAGG751.9999608E-411.4429043
GTTACAC751.9999608E-411.4429043
TCCTACA5350.011.2297822
AGGACCT3800.011.0406475
TAGCACT1053.308016E-610.8972634
GGACGTG3950.010.8627776
TGAGTTA1752.1827873E-1110.83454912
CGTGGAA2900.010.81493959
AGGACGT4000.010.7269915
TGGAATA7250.010.72246711
Produced by FastQC (version 0.11.5)