FastQCFastQC Report
Thu 26 May 2016
SRR1926461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808924
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGC450.001194217717.8020531
CTAGCAC651.3946043E-515.1978173
CTAAGCA505.921967E-415.03585154
ATAGGAC702.8081162E-514.1122593
GTCCTAA2000.014.0191171
TATTGGT600.00680592413.7110162
CTCTATA700.001189645613.7110162
CCTATAC755.3724325E-513.1714423
TAGGACG1650.013.0180544
TCTACTC600.001991502712.8055683
TATACTG1151.8811988E-812.7148845
ACGTGGA852.867022E-612.6462838
GTATTGG854.3659224E-412.5661551
TAAGGGA600.002345347512.5298764
CTCTAGT750.002442507812.4614371
CTATACT1305.287802E-912.3921864
TGTAGAA1009.8842815E-512.3399142
AGGACGT1800.012.1850975
GTCCTAC2550.012.0425661
CCTACAC1202.3501889E-711.8908843
Produced by FastQC (version 0.11.5)