##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926461_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 808924 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.77624226750597 27.0 7.0 30.0 2.0 31.0 2 16.212516874267546 16.0 2.0 30.0 2.0 31.0 3 19.918030618451176 23.0 15.0 31.0 2.0 31.0 4 22.193796945077658 28.0 2.0 35.0 2.0 37.0 5 22.532993210734258 29.0 2.0 35.0 2.0 37.0 6 23.417089862582888 30.0 2.0 35.0 2.0 37.0 7 23.32314655023216 30.0 7.0 35.0 2.0 37.0 8 23.46758409937151 31.0 7.0 35.0 2.0 37.0 9 26.122250050684613 31.0 17.0 37.0 2.0 39.0 10 26.79513650231666 32.0 23.0 37.0 2.0 39.0 11 23.9341817031019 30.0 7.0 37.0 2.0 39.0 12 25.033751007511214 31.0 10.0 37.0 2.0 39.0 13 21.714927483916906 27.0 2.0 35.0 2.0 39.0 14 25.05926391107199 31.0 16.0 38.0 2.0 40.0 15 26.118080314096257 31.0 17.0 38.0 2.0 40.0 16 26.509579391883538 31.0 17.0 39.0 2.0 40.0 17 25.808904173939702 31.0 10.0 38.0 2.0 40.0 18 25.8775274315016 31.0 11.0 38.0 2.0 40.0 19 26.27304419203782 31.0 16.0 39.0 2.0 40.0 20 26.499196463450215 31.0 12.0 39.0 2.0 40.0 21 24.366763750364683 30.0 2.0 37.0 2.0 40.0 22 25.289066710840572 31.0 2.0 38.0 2.0 40.0 23 25.285757376465526 31.0 2.0 38.0 2.0 40.0 24 24.36594785171413 31.0 2.0 38.0 2.0 40.0 25 24.15691708986258 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.664105011113321 1101 2 10.508927157097782 1101 3 7.459590643350875 1101 4 8.803639339256335 1101 5 8.435036234980277 1101 6 8.015521242279373 1101 7 7.7323918937368745 1101 8 6.283304990586398 1101 9 6.035882177054319 1101 10 5.6723577220723485 1101 11 8.496712602505006 1101 12 6.065196844329737 1101 13 10.566989982338683 1101 14 7.583114401774413 1101 15 6.869809577187915 1101 16 6.484129358815089 1101 17 2.1100749762141007 1101 18 5.165195659889367 1101 19 5.623587886234091 1101 20 3.6966654993336654 1101 21 0.8165077677456374 1101 22 4.477100833046645 1101 23 5.658531952255107 1101 24 6.9769163859741 1101 25 7.543351901140888 1102 1 5.675000480863567 1102 2 10.924648908495117 1102 3 7.174561093597212 1102 4 8.598612140658478 1102 5 8.44556834428765 1102 6 7.231622384727352 1102 7 7.249927241227017 1102 8 7.541553806993189 1102 9 6.515863154066427 1102 10 5.7777536246490016 1102 11 8.539538905526243 1102 12 5.773430164482349 1102 13 10.302371671109778 1102 14 8.010301243851547 1102 15 5.858849985006579 1102 16 6.438026399564897 1102 17 7.163338273603248 1102 18 7.1509262815563375 1102 19 6.0056592544999 1102 20 6.242131509101654 1102 21 5.13425258184402 1102 22 6.468603542219359 1102 23 6.942773864813486 1102 24 8.284758559297028 1102 25 8.176537396646975 1103 1 4.88180281920733 1103 2 9.342350801632891 1103 3 6.769889307807574 1103 4 8.414314123306315 1103 5 7.793561081185615 1103 6 7.232992741126576 1103 7 7.418743214936633 1103 8 6.99308091255752 1103 9 6.082713316356692 1103 10 5.363063105682457 1103 11 6.264907122208786 1103 12 5.092528201441478 1103 13 9.041130166573534 1103 14 7.517949026026688 1103 15 6.569206085863108 1103 16 6.127489360515064 1103 17 3.677209136160549 1103 18 2.7630791903136185 1103 19 4.931514022295886 1103 20 5.3203142843823805 1103 21 7.709492181094692 1103 22 3.104180381278141 1103 23 5.3199042575282185 1103 24 6.837533264606471 1103 25 2.7362693779521585 1104 1 5.592018211234109 1104 2 10.404071736791026 1104 3 7.219079173813093 1104 4 7.876062467842193 1104 5 7.713855856584452 1104 6 7.446652993583555 1104 7 7.559334634555519 1104 8 7.6483955286082015 1104 9 6.408755132269494 1104 10 5.44641876104691 1104 11 8.4467797641198 1104 12 5.161078639887698 1104 13 9.54969248241678 1104 14 7.403872026439906 1104 15 6.649243545346025 1104 16 6.39626426515327 1104 17 6.65140071525218 1104 18 4.241469387723431 1104 19 5.037799075671494 1104 20 4.303760681398913 1104 21 3.680048902933116 1104 22 5.581850044288146 1104 23 5.956537929118191 1104 24 6.834192227576818 1104 25 5.701375371261779 1105 1 3.4413222497926235 1105 2 9.441136959875605 1105 3 6.5450220570129645 1105 4 6.0353304681995965 1105 5 2.460531129695269 1105 6 4.94340298254307 1105 7 5.863221193816226 1105 8 2.9970217326630895 1105 9 3.788505237282493 1105 10 3.539988165651458 1105 11 6.612890811897891 1105 12 4.003554009333179 1105 13 5.0774118055271344 1105 14 3.7497437575573365 1105 15 4.289076181844944 1105 16 4.509607437357129 1105 17 5.20269635482498 1105 18 2.7599306212384462 1105 19 2.5126663074056523 1105 20 1.6414054529840918 1105 21 2.7852354666100183 1105 22 3.8999839658996684 1105 23 1.4693284607971968 1105 24 4.555257680714316 1105 25 5.105321600499245 1106 1 6.270117733863394 1106 2 10.858384809097947 1106 3 7.7608690350647755 1106 4 9.059952800089022 1106 5 8.499633755675138 1106 6 7.694634727578887 1106 7 7.711947820825731 1106 8 7.494267148560542 1106 9 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1.2504804208829157 1107 23 -1.5531907270670686 1107 24 1.7708990018467894 1107 25 2.6412472352823855 1108 1 5.213577005494642 1108 2 10.59785283497673 1108 3 0.34443274333331786 1108 4 6.0214585882592715 1108 5 7.488320906513124 1108 6 7.331757728596376 1108 7 7.45094357808734 1108 8 7.061780923061878 1108 9 5.7428252628144705 1108 10 4.982749775650493 1108 11 7.142492320065898 1108 12 2.38478948987618 1108 13 3.245483473195783 1108 14 4.965646320403856 1108 15 4.8992800665650975 1108 16 4.8690115922412325 1108 17 0.23423280098145227 1108 18 3.6591505599320087 1108 19 4.762710468821194 1108 20 4.992188112020852 1108 21 4.840158907786272 1108 22 1.9832211832109437 1108 23 4.791637026627395 1108 24 5.352422443615758 1108 25 5.934560168630291 1109 1 3.228402377938245 1109 2 -13.710050945091998 1109 3 -5.840353932392123 1109 4 1.5195680615703502 1109 5 1.3125329408970785 1109 6 2.2427690619100176 1109 7 3.215417430724724 1109 8 3.3499301762053726 1109 9 2.966218068966562 1109 10 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2204 7 5.29091245757829 2204 8 5.209857963394157 2204 9 3.7279498546016825 2204 10 3.35034542365705 2204 11 4.727278878737568 2204 12 5.009941007244887 2204 13 2.9469182847532984 2204 14 4.573009393822403 2204 15 4.5747175462916445 2204 16 4.353023796553714 2204 17 -0.7232374625917011 2204 18 2.8909207949698974 2204 19 3.750190073424882 2204 20 3.8178230216226012 2204 21 5.320670786504085 2204 22 4.984142116143321 2204 23 3.70028077240503 2204 24 5.230822712871575 2204 25 5.350249685457559 2205 1 5.905993862674471 2205 2 10.087020184615115 2205 3 7.268939206402521 2205 4 7.871334215514874 2205 5 7.982323695913404 2205 6 7.654219590017821 2205 7 7.508366402283233 2205 8 7.571111035644375 2205 9 6.38283877846531 2205 10 5.6984854440281865 2205 11 7.887917847391954 2205 12 6.83217910007906 2205 13 10.092441732840065 2205 14 7.743089561859517 2205 15 6.476178368481733 2205 16 6.124036107331314 2205 17 6.939138694667914 2205 18 6.5863055154310075 2205 19 5.185267227365529 2205 20 5.649451336510062 2205 21 -0.8715191896701988 2205 22 3.7907108667434173 2205 23 5.231480877725183 2205 24 7.043723540684013 2205 25 7.438120248573927 2206 1 4.046908880655646 2206 2 8.566469564101778 2206 3 5.882401120436125 2206 4 1.847301776407246 2206 5 5.8238230281538605 2206 6 6.100998069419855 2206 7 6.0149335264194335 2206 8 6.273795290919871 2206 9 4.905617565608814 2206 10 4.12944482807179 2206 11 3.5465654814321077 2206 12 4.92452225549442 2206 13 8.217735911989 2206 14 0.6765306047085744 2206 15 2.608620632433169 2206 16 3.72369305449644 2206 17 4.707434023901975 2206 18 -0.3656305927046084 2206 19 1.3074603193585297 2206 20 2.782116515759604 2206 21 3.6372761010961767 2206 22 -1.634865014215869 2206 23 -2.459586136926074 2206 24 1.7303559217942102 2206 25 3.106120461625718 2207 1 1.5568415370342912 2207 2 -13.710050945091998 2207 3 -6.040593524444143 2207 4 -3.9766583381543157 2207 5 1.3143336996900992 2207 6 1.9255809541475593 2207 7 3.0919740662943163 2207 8 3.2014976560391126 2207 9 0.6509854925934135 2207 10 0.6391452650664498 2207 11 -1.4808152950250708 2207 12 -2.5571429685972866 2207 13 -0.03671671877514271 2207 14 -5.256238365152164 2207 15 -1.3276105173342572 2207 16 -0.3365643571722323 2207 17 0.8319950940814884 2207 18 0.7677548140663433 2207 19 0.0815390065449435 2207 20 0.1161852104755603 2207 21 -3.9305530698806237 2207 22 -4.053732487317124 2207 23 -1.1503086206883104 2207 24 0.4584446155249893 2207 25 1.0127084551798688 2208 1 4.637258877360729 2208 2 6.113041559972148 2208 3 5.9739205743010615 2208 4 8.084100854212199 2208 5 3.4731959909155563 2208 6 6.212929779616051 2208 7 6.470944459817201 2208 8 6.581543505695048 2208 9 4.97467427353677 2208 10 4.404842677493079 2208 11 6.48783667038181 2208 12 5.940351233009707 2208 13 8.504703699150664 2208 14 2.5703473327689537 2208 15 4.359134209883322 2208 16 4.866203514667568 2208 17 5.766540079197387 2208 18 0.632719083104103 2208 19 3.0618131926703605 2208 20 3.9537841358832466 2208 21 6.670428229852025 2208 22 5.57254482679717 2208 23 1.4545373145818026 2208 24 3.1845670658757257 2208 25 4.3388644025578635 2209 1 2.956879209435705 2209 2 4.560767302554636 2209 3 5.002648937087034 2209 4 0.5050764656378313 2209 5 4.712506279431302 2209 6 5.324873927575652 2209 7 5.79656891557126 2209 8 4.593144470070744 2209 9 4.205108433284597 2209 10 3.283567202138098 2209 11 -0.5732562659231384 2209 12 3.2319394459566126 2209 13 1.798734143186902 2209 14 3.2351945087528513 2209 15 3.744117837889725 2209 16 3.371483280917449 2209 17 0.8857204674216774 2209 18 3.120375181763393 2209 19 3.576338722663845 2209 20 3.7158233083898367 2209 21 5.948036808903794 2209 22 4.915312326886401 2209 23 0.6235900662843967 2209 24 -2.664400865356523 2209 25 0.4611206818298008 2210 1 -2.1655477965708094 2210 2 -13.710050945091998 2210 3 -7.744827077685086 2210 4 -2.32258951657354 2210 5 -1.0871832010298164 2210 6 -1.5455282390308973 2210 7 -1.4743758760880823 2210 8 -20.90866581233331 2210 9 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2211 21 -4.9448321309539445 2211 22 -6.638533487622325 2211 23 -8.868476431601902 2211 24 -21.90118580380692 2211 25 -21.68949481418758 2212 1 -1.0191603710571684 2212 2 -13.710050945091998 2212 3 -7.907022319977417 2212 4 -19.67957233957578 2212 5 -19.911679093486875 2212 6 -20.78301890370603 2212 7 -10.15908242729949 2212 8 -9.709716863384362 2212 9 -5.50224609720534 2212 10 -5.692715380227032 2212 11 -5.747839248616188 2212 12 -3.417352858657921 2212 13 -6.137178054311104 2212 14 -3.940831807208294 2212 15 -4.162264467659849 2212 16 -4.204142489675736 2212 17 -4.535797538636519 2212 18 -3.620970798539865 2212 19 -4.012943535698792 2212 20 -4.145292151748073 2212 21 -1.9883912918979512 2212 22 -3.256209484570242 2212 23 -3.256928033277873 2212 24 -2.4214560740771915 2212 25 -3.019074393767159 2213 1 -18.306697908594707 2213 2 -13.710050945091998 2213 3 -8.700359099379769 2213 4 -5.883670700231519 2213 5 -19.911679093486875 2213 6 -10.887936936492915 2213 7 -20.755929274146336 2213 8 -10.870141222169377 2213 9 -8.616965734875798 2213 10 -7.392474155395643 2213 11 -6.952240371050099 2213 12 -5.462055758278854 2213 13 -19.065856732989783 2213 14 -12.415126593798163 2213 15 -7.093227397803108 2213 16 -6.479087106423631 2213 17 -5.07688206578662 2213 18 -4.988860819708574 2213 19 -5.926735196211762 2213 20 -5.861124377416363 2213 21 -4.095805263246348 2213 22 -5.060418611730189 2213 23 -4.789589383152336 2213 24 -3.8298743283970857 2213 25 -4.670642355171186 2214 1 -18.306697908594707 2214 2 -13.710050945091998 2214 3 -12.621417580536816 2214 4 -19.67957233957578 2214 5 -19.911679093486875 2214 6 -20.78301890370603 2214 7 -16.75146897084571 2214 8 -15.450146633029117 2214 9 -14.590154924855472 2214 10 -14.525516959683385 2214 11 -15.068924830183482 2214 12 -13.07368983792086 2214 13 -19.065856732989783 2214 14 -17.02666049941522 2214 15 -23.637489692885076 2214 16 -18.593520940383357 2214 17 -23.32934108218006 2214 18 -18.009536884697386 2214 19 -15.497852173149802 2214 20 -14.267309575058983 2214 21 -11.859492544801165 2214 22 -12.968308961388718 2214 23 -12.871722578045654 2214 24 -11.75310373422619 2214 25 -11.466479649156357 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 149222.0 3 3.0 4 806.0 5 2667.0 6 3455.0 7 3503.0 8 3008.0 9 2990.0 10 2750.0 11 2468.0 12 2479.0 13 2739.0 14 3917.0 15 8094.0 16 11904.0 17 12648.0 18 13366.0 19 15341.0 20 18255.0 21 20904.0 22 21896.0 23 23946.0 24 24860.0 25 25393.0 26 21266.0 27 17020.0 28 18005.0 29 20976.0 30 20423.0 31 23496.0 32 30207.0 33 37758.0 34 45768.0 35 52710.0 36 51864.0 37 54648.0 38 38169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60480609428162 17.24273700578472 11.852607402440851 26.29984949749281 2 15.370106692153154 19.56757494782422 39.41387583139909 25.648442528623534 3 19.566252793089014 22.827557341149827 28.61225298465261 28.99393688110855 4 11.863036749039495 15.473906975942848 37.22225731793461 35.44079895708305 5 13.767382896617642 36.67524692691707 35.56906584860872 13.98830432785656 6 34.09645315012255 35.870314212694694 17.091984334431395 12.94124830275136 7 29.213097002865258 30.799831439298377 21.270560382507327 18.71651117532904 8 26.02265567397279 36.267176084187945 19.843957369796904 17.866210872042362 9 26.270053806820147 15.293239744776482 19.089169830560493 39.34753661784288 10 14.18075571960536 29.308953446196362 34.226389566967924 22.283901267230355 11 34.68326056115291 21.924177818724434 23.833184376751223 19.559377243371433 12 23.532735232749424 24.226358471427297 30.924506882361293 21.31639941346199 13 28.547036824785387 20.54253490171983 25.28978914221232 25.62063913128246 14 20.773961471605197 21.02244661717074 27.31149630288793 30.892095608336128 15 23.208845202583632 29.85281356597395 23.076325168485205 23.86201606295721 16 22.99932073442245 27.767178855851686 26.1477740352326 23.08572637449326 17 22.186881006821267 27.51791298587979 27.723506295817437 22.571699711481507 18 22.09486756692608 27.229435544130336 28.713695655722987 21.9620012332206 19 23.210343265167293 26.834527588357926 26.82109771051961 23.134031435955173 20 23.543368874376604 26.988631080995646 27.19738977255964 22.27061027206811 21 22.884930971016765 25.76576903299843 26.533837089268157 24.815462906716647 22 22.92456530265394 27.043504932771505 27.443506944095258 22.5884228204793 23 22.668305136553595 26.789725160289134 27.448206902611282 23.093762800545992 24 22.84957439564181 26.57473612529792 27.49894450119169 23.076744977868575 25 22.948293849243917 26.776165247813555 27.253612723022297 23.021928179920227 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 617.0 1 617.0 2 1463.5 3 2310.0 4 2310.0 5 2310.0 6 3450.0 7 4590.0 8 4590.0 9 4590.0 10 5226.0 11 5862.0 12 5862.0 13 5862.0 14 6854.5 15 7847.0 16 7847.0 17 7847.0 18 10701.0 19 13555.0 20 13555.0 21 13555.0 22 19400.0 23 25245.0 24 25245.0 25 25245.0 26 33556.0 27 41867.0 28 41867.0 29 41867.0 30 51676.0 31 61485.0 32 61485.0 33 61485.0 34 70624.0 35 79763.0 36 79763.0 37 79763.0 38 86769.5 39 93776.0 40 93776.0 41 93776.0 42 97605.0 43 101434.0 44 101434.0 45 101434.0 46 101720.5 47 102007.0 48 102007.0 49 102007.0 50 96574.0 51 91141.0 52 91141.0 53 91141.0 54 80970.5 55 70800.0 56 70800.0 57 70800.0 58 60154.0 59 49508.0 60 49508.0 61 49508.0 62 39783.0 63 30058.0 64 30058.0 65 30058.0 66 22888.5 67 15719.0 68 15719.0 69 15719.0 70 11427.0 71 7135.0 72 7135.0 73 7135.0 74 4933.5 75 2732.0 76 2732.0 77 2732.0 78 1873.0 79 1014.0 80 1014.0 81 1014.0 82 677.5 83 341.0 84 341.0 85 341.0 86 218.0 87 95.0 88 95.0 89 95.0 90 57.5 91 20.0 92 20.0 93 20.0 94 11.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.343527945764992 2 36.620745583021396 3 5.782372633275808 4 13.89697919705683 5 13.602390335804104 6 14.963828493158813 7 9.352794576499152 8 7.784909336352983 9 0.10483061449530488 10 1.5016243800406466 11 5.804871656669848 12 5.915017974494514 13 16.30313849014246 14 1.644530264895095 15 2.983840261878743 16 2.998180298767251 17 2.9519460419025765 18 1.5619514317785106 19 1.5073109463929864 20 0.27827088824166424 21 4.556299479308316 22 1.6599828908525398 23 0.10544871953360266 24 9.231275125969807 25 0.10841562371743205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 808924.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.02168618061188 #Duplication Level Percentage of deduplicated Percentage of total 1 96.99965709289422 92.17070975907959 2 2.3591072592256066 4.48332699305078 3 0.34691651082527136 0.9889377546753534 4 0.11747349386914364 0.446501178758952 5 0.05663469088980852 0.2690761912333672 6 0.032848220298463224 0.18727759684753065 7 0.02168468011258886 0.14423604080097618 8 0.0147133038992185 0.11184663566332108 9 0.007284426567514898 0.06229606457737011 >10 0.039587766409147365 0.6792015080382606 >50 0.002376558490238194 0.15368077511651557 >100 0.0017159965188104096 0.3029095021580283 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTGC 45 0.0011942177 17.802053 1 CTAGCAC 65 1.3946043E-5 15.197817 3 CTAAGCA 50 5.921967E-4 15.0358515 4 ATAGGAC 70 2.8081162E-5 14.112259 3 GTCCTAA 200 0.0 14.019117 1 TATTGGT 60 0.006805924 13.711016 2 CTCTATA 70 0.0011896456 13.711016 2 CCTATAC 75 5.3724325E-5 13.171442 3 TAGGACG 165 0.0 13.018054 4 TCTACTC 60 0.0019915027 12.805568 3 TATACTG 115 1.8811988E-8 12.714884 5 ACGTGGA 85 2.867022E-6 12.646283 8 GTATTGG 85 4.3659224E-4 12.566155 1 TAAGGGA 60 0.0023453475 12.529876 4 CTCTAGT 75 0.0024425078 12.461437 1 CTATACT 130 5.287802E-9 12.392186 4 TGTAGAA 100 9.8842815E-5 12.339914 2 AGGACGT 180 0.0 12.185097 5 GTCCTAC 255 0.0 12.042566 1 CCTACAC 120 2.3501889E-7 11.890884 3 >>END_MODULE