FastQCFastQC Report
Thu 26 May 2016
SRR1926461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808924
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2450.013.6222244
AATCCCG500.00153013113.26313519
GAACCAT655.2615782E-513.2022576
GTAGGAC4950.012.9091233
GTCTAGG905.159054E-712.71328451
TGTAGGA5400.012.3639832
CTGTAGG5400.012.3601371
TTGCACT701.0522896E-412.2600014
CCCTAAG907.1494687E-611.6538441
GTCCTAT752.0019515E-411.4419561
AGGACGT3950.011.3461055
TAGGACG3750.011.1883874
TATTGCA700.001441322510.9004922
CTAGGAC1407.825292E-910.8998143
CTTATAC1158.361276E-710.7813383
GGACGTG4000.010.7268336
GACGTGA2050.010.6612847
TCTATAC1355.350921E-810.597043
TGGCGAG3700.010.49784818
CAAGACA1553.428795E-910.4583524
Produced by FastQC (version 0.11.5)