FastQCFastQC Report
Thu 26 May 2016
SRR1926460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472869
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA38440.8129101294438841No Hit
GCTTACTCTGCGTTGATACCACTGC32780.693215245660003No Hit
CTGCTTACTCTGCGTTGATACCACT23410.4950631147315641No Hit
GCGTTGATACCACTGCTTACTCTGC15740.3328617439502272No Hit
GNGTAAGCAGTGGTATCAACGCAGA9090.19223082925715157No Hit
GTATCAACGCAGAGTAAGCAGTGGT8150.172352173646401No Hit
GNTTACTCTGCGTTGATACCACTGC7690.16262432090071458No Hit
ACGCAGAGTAAGCAGTGGTATCAAC6310.13344076266365526No Hit
GTACATGGGAAGCAGTGGTATCAAC6000.12688503581330138No Hit
GAGTACATGGGAAGCAGTGGTATCA5560.11758013318699258No Hit
TGCTTACTCTGCGTTGATACCACTG5540.11715718306761491No Hit
ACTCTGCGTTGATACCACTGCTTAC5350.11313915693352704No Hit
CNGCTTACTCTGCGTTGATACCACT5350.11313915693352704No Hit
CCACTGCTTACTCTGCGTTGATACC5100.10785228044130615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG350.001175436517.8423866
GTATTTG450.00120864717.7663761
GTCCTAA858.5341526E-817.2438351
TGTAGGA1750.017.1742292
TAGGACC659.621253E-716.4772514
CAATACT400.002371699316.065324
GTGTAGG500.002445865715.9897381
CTGTAGG1600.015.823181
TGCTATC1003.6379788E-1215.61926511
TCCTACA2050.015.32733252
GCTATCA1200.015.07617712
GTCCTAC1950.015.0330881
GTAGGAC1700.014.7829983
GTGCTAT1250.014.70048610
TTACTCG507.477815E-414.5776085
AGTGCTA1200.014.5473559
GTCCTAG550.004613383714.5361271
TCCTAAA1057.0831265E-714.3118572
GGGTATC1001.0786607E-913.78170411
TAGGACG1252.0918378E-1013.7090724
Produced by FastQC (version 0.11.5)