FastQCFastQC Report
Thu 26 May 2016
SRR1926460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472869
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA101332.1428767798269712No Hit
GCTTACTCTGCGTTGATACCACTGC74471.5748547695027586No Hit
CTGCTTACTCTGCGTTGATACCACT56241.1893357356900114No Hit
GCGTTGATACCACTGCTTACTCTGC34690.7336069820605707No Hit
GTATCAACGCAGAGTAAGCAGTGGT20410.43162059682491344No Hit
ACGCAGAGTAAGCAGTGGTATCAAC18160.38403870839492543No Hit
GTACATGGGAAGCAGTGGTATCAAC15110.31953881518983057No Hit
GAGTACATGGGAAGCAGTGGTATCA13690.2895093567140159No Hit
CCACTGCTTACTCTGCGTTGATACC13400.2833765799830397No Hit
ACTCTGCGTTGATACCACTGCTTAC12990.2747061025357974No Hit
GTGGTATCAACGCAGAGTAAGCAGT12030.25440449680566923No Hit
TATCAACGCAGAGTAAGCAGTGGTA11770.24890614525375948No Hit
GTAAGCAGTGGTATCAACGCAGAGT11480.24277336852278328No Hit
AAGCAGTGGTATCAACGCAGAGTAA11120.23516026637398516No Hit
GGTATCAACGCAGAGTAAGCAGTGG10680.2258553637476764No Hit
GAGCAAGCAGTGGTATCAACGCAGA9360.19794065586875012No Hit
CAGTGGTATCAACGCAGAGTAAGCA9010.19053902877964088No Hit
TGCTTACTCTGCGTTGATACCACTG9000.19032755371995203No Hit
GCAGTGGTATCAACGCAGAGTAAGC8090.171083323288268No Hit
GATACCACTGCTTACTCTGCGTTGA7800.16495054655729174No Hit
GCTTTCTCTGCGTTGATACCACTGC7300.15437679357284997No Hit
CCCATGTACTCTGCGTTGATACCAC7300.15437679357284997No Hit
ACTGCTTACTCTGCGTTGATACCAC7190.1520505679162728No Hit
ATACCACTGCTTACTCTGCGTTGAT6890.14570631612560772No Hit
GAGAAAGCAGTGGTATCAACGCAGA6730.14232271517058637No Hit
GTTGATACCACTGCTTACTCTGCGT6720.1421112401108975No Hit
TACCACTGCTTACTCTGCGTTGATA6100.12899978641018972No Hit
GCTTCCCATGTACTCTGCGTTGATA6090.1287883113505009No Hit
GCTTCCTCTGCGTTGATACCACTGC5880.12434733509703534No Hit
CTGTAGGACGTGGAATATGGCAAGA5460.11546538259010422No Hit
GTCCTACAGTGTGCATTTCTCATTT5300.11208178163508287No Hit
GTCCTACAGTGGACATTTCTAAATT5240.11081293127694984No Hit
GTATCAACGCAGAGTACTTTTTTTT5240.11081293127694984No Hit
AGAGTAAGCAGTGGTATCAACGCAG5190.10975555597850567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG601.4120378E-615.8857161
AGTACGC759.2127266E-713.9883582
TAGGACC1550.013.5356794
GTGTAAT500.001460037513.3440011
GTATAAC500.001460037513.3440011
CCTCGAA500.00152566513.26639617
GTATAAA655.2708212E-513.1973641
ACTGTCT604.0822584E-412.6668088
GGCGAGG1457.2759576E-1212.41682319
CGCAGTG853.7664722E-612.3400416
AGACCAT550.00297624312.1373655
CGTTAAT959.865416E-712.0473882
GTAGGAC4050.012.0089263
GTACGCA802.746464E-511.9206263
TAGGACA1458.913048E-1111.8384154
CTAAATT1451.0004442E-1011.76330719
TGTAGGA4250.011.6694312
TAATACC907.1189916E-611.6557245
GCGTTAA907.161305E-611.6495251
GGACCTG1900.011.5429576
Produced by FastQC (version 0.11.5)