FastQCFastQC Report
Thu 26 May 2016
SRR1926459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635173
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7740.12185656506180206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGG552.3755924E-416.9499841
TGTAGGA4650.016.4300462
TAGGACC2350.016.3769114
GTCCTAG901.6514787E-716.2773651
GTTCAGA901.6514787E-716.2773651
CTGTAGG4750.015.9814121
AGGACTG601.0951764E-515.5894755
CTAGGAC1001.4424586E-915.2601423
TCCTACA6950.015.1150522
GTCCTAC6200.015.0362751
TACTATA550.00394731914.8830622
GGAGTAC450.004625214314.5334693
GTCTAGG658.4600056E-414.3422931
GTAGGAC5650.013.8903953
GGACTGT601.9143913E-413.8528456
TCTTACA700.001234409513.6428072
TGGACTG851.4336529E-613.4497435
GTCTAAG600.00824630913.3178431
GTAGTAG600.00824630913.3178431
TCTAGGA1253.576297E-713.0970952
Produced by FastQC (version 0.11.5)