##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926459_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 635173 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.920586989686274 27.0 7.0 30.0 2.0 31.0 2 16.341085027228804 16.0 2.0 30.0 2.0 31.0 3 20.035289598266928 24.0 15.0 31.0 2.0 31.0 4 22.30079679079558 28.0 2.0 35.0 2.0 37.0 5 22.63474675403394 29.0 2.0 35.0 2.0 37.0 6 23.522991374003617 31.0 2.0 35.0 2.0 37.0 7 23.431861870702942 31.0 7.0 35.0 2.0 37.0 8 23.598817959831415 31.0 8.0 35.0 2.0 37.0 9 26.216964512030582 32.0 17.0 37.0 2.0 39.0 10 26.848666426312203 32.0 23.0 37.0 2.0 39.0 11 24.02207115226875 30.0 7.0 37.0 2.0 39.0 12 25.08375985755062 31.0 10.0 37.0 2.0 39.0 13 21.776002758303644 27.0 2.0 35.0 2.0 39.0 14 25.109278889373446 31.0 17.0 38.0 2.0 40.0 15 26.193375663008347 31.0 17.0 38.0 2.0 40.0 16 26.58112514228407 31.0 17.0 38.0 2.0 40.0 17 25.858015060463842 31.0 11.0 38.0 2.0 40.0 18 25.910490527777473 31.0 12.0 38.0 2.0 40.0 19 26.31251958127943 31.0 16.0 39.0 2.0 40.0 20 26.5502107299901 31.0 16.0 39.0 2.0 40.0 21 24.412752116352554 30.0 2.0 37.0 2.0 40.0 22 25.33583448918641 31.0 2.0 38.0 2.0 40.0 23 25.336439048889044 31.0 2.0 38.0 2.0 40.0 24 24.414757869115974 31.0 2.0 38.0 2.0 40.0 25 24.21341114940339 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.6963338170787345 1101 2 10.502001758113886 1101 3 7.406861034232531 1101 4 9.00644935633622 1101 5 8.388688311701195 1101 6 8.190381946675185 1101 7 7.874305895298438 1101 8 6.31979945916952 1101 9 6.228027422999315 1101 10 5.850655219091944 1101 11 8.62642206917987 1101 12 6.146593161132447 1101 13 10.521980489814812 1101 14 7.977101633936812 1101 15 7.213317928422008 1101 16 6.859793528159905 1101 17 2.096838383988935 1101 18 5.026308725086455 1101 19 5.66836835529519 1101 20 3.511667249871735 1101 21 0.7671029030117253 1101 22 4.927504321756231 1101 23 6.188491301258754 1101 24 7.53899811083356 1101 25 8.01987516636595 1102 1 5.824670447539216 1102 2 10.90691385546001 1102 3 7.491294861512838 1102 4 8.816614461533238 1102 5 8.218800414024116 1102 6 7.0981494805485 1102 7 7.738693189539042 1102 8 7.689418609879336 1102 9 6.505904321957921 1102 10 5.578536472746784 1102 11 8.176367946526739 1102 12 5.493515635097584 1102 13 10.209258555832008 1102 14 7.754302932880485 1102 15 5.4509225966563655 1102 16 5.992589367558708 1102 17 6.720246742517293 1102 18 6.921009681875425 1102 19 5.52033107909547 1102 20 5.834536870266646 1102 21 5.009460411130124 1102 22 6.146241957529437 1102 23 6.704711922611697 1102 24 8.045932031428784 1102 25 8.016971206080779 1103 1 4.3771169834611605 1103 2 9.159800587170531 1103 3 6.329522087950863 1103 4 7.7721210151944184 1103 5 7.059896304435075 1103 6 6.648106774986921 1103 7 6.652701434997468 1103 8 6.176654574532272 1103 9 5.115913027322396 1103 10 4.424496690873649 1103 11 5.021791060090681 1103 12 3.9567428615356093 1103 13 8.36861353760002 1103 14 6.464902946020658 1103 15 5.443991155507916 1103 16 5.011843005744051 1103 17 2.9962757822991684 1103 18 1.2979404660152376 1103 19 3.457653276132362 1103 20 3.8088847891917794 1103 21 6.207720980756616 1103 22 1.5621423878700966 1103 23 4.017196729286905 1103 24 5.485188297648683 1103 25 1.1151956402320877 1104 1 5.373620137761986 1104 2 10.258829749211104 1104 3 6.905359277253837 1104 4 7.570437491362281 1104 5 7.444374448948903 1104 6 7.251917927670483 1104 7 7.227847116846281 1104 8 7.12731367258835 1104 9 6.067827588447802 1104 10 5.061687116944924 1104 11 8.113812090902073 1104 12 4.8041010142640985 1104 13 9.175059638524807 1104 14 6.812857142877267 1104 15 6.03289238064734 1104 16 5.885629257741559 1104 17 6.304890264486833 1104 18 3.7934881848428503 1104 19 4.38410078895172 1104 20 3.9917676532840893 1104 21 2.7273126223407615 1104 22 5.0568004526760575 1104 23 5.53650920080835 1104 24 6.56845798876466 1104 25 5.399238343625129 1105 1 3.1968845736443328 1105 2 9.654302857694537 1105 3 6.576588264796623 1105 4 6.032523331639137 1105 5 2.5191161625911036 1105 6 4.938587156639045 1105 7 6.1608587389415455 1105 8 3.4015384971813845 1105 9 4.453436625465155 1105 10 3.763212951907377 1105 11 7.152256900184565 1105 12 4.333241662494842 1105 13 5.478485214726401 1105 14 3.9259851440919853 1105 15 4.402770940190809 1105 16 4.658240781140755 1105 17 5.341656924194162 1105 18 2.980972763581441 1105 19 2.6124762262828796 1105 20 1.8938125916306774 1105 21 2.908311572113316 1105 22 4.211786502365733 1105 23 1.9791144291695524 1105 24 4.840046963907167 1105 25 5.674847918409547 1106 1 6.484786265442327 1106 2 11.39210408717692 1106 3 8.060742617743728 1106 4 9.360613098969672 1106 5 8.581210691646444 1106 6 8.002972793362275 1106 7 7.837360634813461 1106 8 7.6224453664103144 1106 9 6.760016749673113 1106 10 5.8841440706607635 1106 11 8.424117689018445 1106 12 5.906657885111777 1106 13 4.873780806981671 1106 14 6.145265405608935 1106 15 6.453485513840079 1106 16 6.457886171211676 1106 17 6.984966889803925 1106 18 6.918635739281527 1106 19 6.921405720003197 1106 20 6.402644772324276 1106 21 6.509326587922747 1106 22 6.99693256638448 1106 23 7.405995814352778 1106 24 8.169797981127477 1106 25 7.10342991959368 1107 1 2.9530292695053433 1107 2 -13.727789340887023 1107 3 -5.990848599626057 1107 4 0.8744217143176627 1107 5 3.493758738297508 1107 6 3.857904016558745 1107 7 3.895229634659959 1107 8 4.223210980201387 1107 9 2.4326009468280603 1107 10 2.1137373618289494 1107 11 4.743662769072703 1107 12 2.2776861069392886 1107 13 3.9429397908831056 1107 14 2.7084113139295027 1107 15 2.6061978980503895 1107 16 2.3044470177725245 1107 17 3.143155104238442 1107 18 -2.1049404038920123 1107 19 0.7592453358179441 1107 20 1.1302895701289337 1107 21 -2.3202851857387614 1107 22 0.7856635709236528 1107 23 -2.258103665318046 1107 24 1.2854623641098009 1107 25 2.0295657314606927 1108 1 5.277482737369663 1108 2 10.784699892532442 1108 3 0.5080234728894268 1108 4 6.375693196209298 1108 5 7.651627570584946 1108 6 7.65545128697423 1108 7 7.837233654183361 1108 8 7.342771293560151 1108 9 5.84753470672306 1108 10 4.799578795732398 1108 11 7.192393337958311 1108 12 2.6876774936748618 1108 13 3.8753871884467834 1108 14 5.213661303675618 1108 15 5.090345389949821 1108 16 5.154268600152246 1108 17 0.47899038581015674 1108 18 4.000490054251625 1108 19 5.139520960279615 1108 20 5.494938271986939 1108 21 5.288364992678858 1108 22 2.0100391092526806 1108 23 5.082017740694834 1108 24 5.852584303324765 1108 25 6.52271613546381 1109 1 3.3582320913748305 1109 2 -13.727789340887023 1109 3 -5.6855831211149646 1109 4 2.343360264396562 1109 5 1.7083925450365776 1109 6 2.752443606273772 1109 7 3.822246250273068 1109 8 4.032850820542983 1109 9 3.3986387264623303 1109 10 2.467526928337815 1109 11 1.2356102303184073 1109 12 1.7085549457028648 1109 13 2.896051737416105 1109 14 3.313303173207327 1109 15 3.4275336448247344 1109 16 3.1984876749710764 1109 17 3.8649132031895235 1109 18 3.9451384970640824 1109 19 2.508479532420562 1109 20 1.7643399739161794 1109 21 0.674075716516878 1109 22 3.136958474850136 1109 23 3.6354960747729237 1109 24 4.4881565996987725 1109 25 5.12942185324664 1110 1 3.218277753475288 1110 2 8.977233490163206 1110 3 6.207276596672653 1110 4 7.475061950117713 1110 5 8.121083519046948 1110 6 3.8762316723293218 1110 7 6.125907801411188 1110 8 6.930514500290297 1110 9 5.220059573099473 1110 10 4.8789930937513475 1110 11 3.1891533346545913 1110 12 5.544657187608998 1110 13 9.950811067247532 1110 14 7.415490147492356 1110 15 6.515762779304772 1110 16 6.262909002261907 1110 17 6.953123454846061 1110 18 6.786763106532959 1110 19 5.7340297092324555 1110 20 5.882938696750664 1110 21 4.325442146289941 1110 22 6.243045610497475 1110 23 6.532109182885669 1110 24 7.407575867045221 1110 25 7.893683534847902 1111 1 5.064020605504275 1111 2 9.673304551546886 1111 3 7.4767212839268105 1111 4 7.812970650891515 1111 5 8.401737690032157 1111 6 7.52584560488927 1111 7 7.474429922763317 1111 8 7.691864466782555 1111 9 6.30962888417201 1111 10 5.709986712712148 1111 11 7.495270890629168 1111 12 6.733272048395786 1111 13 8.547850731254393 1111 14 6.452054792455996 1111 15 5.756117753227006 1111 16 5.467457730008395 1111 17 6.757508181976508 1111 18 6.725304500038703 1111 19 5.314615199756634 1111 20 5.225761588572695 1111 21 7.924733696928254 1111 22 7.290322742401418 1111 23 7.05829475332893 1111 24 7.018847787559036 1111 25 7.368596019290738 1112 1 -1.8537932250808673 1112 2 7.789389682621115 1112 3 6.055553740025228 1112 4 -0.18291983458506778 1112 5 4.000221801722596 1112 6 3.1297991787492627 1112 7 5.555209988733143 1112 8 5.826631157461225 1112 9 4.696023803077921 1112 10 3.787492189592218 1112 11 0.56027473969311 1112 12 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0.9199859905337284 2204 6 3.9114467756321467 2204 7 5.148587327180582 2204 8 5.019311928469751 2204 9 3.4474814049073217 2204 10 3.3121769502535905 2204 11 4.863691082673512 2204 12 5.126814158757924 2204 13 2.8876048916020167 2204 14 4.1348750400942755 2204 15 4.270645347849111 2204 16 4.021563301256521 2204 17 -1.0566519203817357 2204 18 2.526493229185867 2204 19 3.5421219343896126 2204 20 3.6073935158427446 2204 21 5.005467536874949 2204 22 4.878588334252004 2204 23 3.480320579982134 2204 24 5.143587497571257 2204 25 5.185871834702521 2205 1 6.2958984622710155 2205 2 10.596966648465239 2205 3 7.610376109259107 2205 4 8.264745637767877 2205 5 8.452975275153797 2205 6 7.945463033724387 2205 7 8.306262969088742 2205 8 7.861494635048189 2205 9 6.5813022953223665 2205 10 6.023269934922599 2205 11 8.180258341279039 2205 12 7.255897681424155 2205 13 10.446143971105919 2205 14 8.016894449534796 2205 15 6.928105727204596 2205 16 6.642890607768905 2205 17 7.320080624849265 2205 18 7.101118509206238 2205 19 5.846218289250526 2205 20 6.154657771515769 2205 21 -0.054535407566621785 2205 22 4.603123888383969 2205 23 5.835222876248661 2205 24 7.688005990255913 2205 25 8.241211420332053 2206 1 3.8452821352729885 2206 2 8.327522336022545 2206 3 5.495931629325668 2206 4 1.50938028591656 2206 5 5.357525803996094 2206 6 5.703960073033297 2206 7 5.723127183950545 2206 8 5.757046626123657 2206 9 4.5463683996356075 2206 10 3.435797307974351 2206 11 3.3970910834609214 2206 12 4.363824044701456 2206 13 7.490719101612374 2206 14 -0.30579945606119097 2206 15 1.9044607078730493 2206 16 3.05885193064978 2206 17 4.317496593183385 2206 18 -1.1607715810284276 2206 19 0.827840269645538 2206 20 2.242629365085058 2206 21 3.2853212359343864 2206 22 -2.461092843084735 2206 23 -3.345886816168214 2206 24 0.6450091679059469 2206 25 2.532434983086162 2207 1 1.9062747960011635 2207 2 -13.727789340887023 2207 3 -5.993415647270211 2207 4 -4.030770123851639 2207 5 1.6372984396663917 2207 6 2.2375163860880747 2207 7 3.300967519638654 2207 8 3.442003149764613 2207 9 1.0232039205864325 2207 10 0.9583525243563287 2207 11 -1.0405985300165916 2207 12 -1.8061414929025474 2207 13 0.5605187323054643 2207 14 -5.285919527672988 2207 15 -1.146864985202292 2207 16 0.1838648188985097 2207 17 1.198181873084902 2207 18 1.5073224266389076 2207 19 0.8248509770463635 2207 20 0.6463838291057549 2207 21 -3.2291565634713493 2207 22 -3.6492909565575644 2207 23 -0.6736107670647691 2207 24 1.2044591443165444 2207 25 1.553999757080959 2208 1 4.855029700256129 2208 2 6.208162836738937 2208 3 6.221854933885901 2208 4 8.057523180225846 2208 5 3.6841490798407115 2208 6 6.138235423562509 2208 7 6.6882418327390525 2208 8 6.822602205837988 2208 9 4.95429211056306 2208 10 4.421093862325218 2208 11 6.555350122319975 2208 12 6.104519772372441 2208 13 8.401874631542967 2208 14 3.1210698144046845 2208 15 4.629714481202612 2208 16 5.0279117903406245 2208 17 5.82552459503378 2208 18 0.942217017447021 2208 19 3.496016692999209 2208 20 4.537861897854977 2208 21 7.340871334939536 2208 22 6.202733501657118 2208 23 2.115005587238972 2208 24 3.565117522501083 2208 25 4.845179543800949 2209 1 3.064832453038484 2209 2 4.829172684429432 2209 3 4.9967833964447586 2209 4 0.5928487006771377 2209 5 5.053567190511025 2209 6 5.6397449292013455 2209 7 6.2125336777024 2209 8 4.8040271791610465 2209 9 4.495300475772314 2209 10 3.5234777230461027 2209 11 -0.2824015713376298 2209 12 3.1780276781669343 2209 13 2.2390016755525863 2209 14 3.7560080366317123 2209 15 4.237446084150896 2209 16 3.778862663074243 2209 17 1.4104066837263076 2209 18 3.3090863413583627 2209 19 3.8135618584888995 2209 20 4.0306981161071 2209 21 6.252951703953826 2209 22 4.991008156015074 2209 23 0.8184931243589126 2209 24 -2.714839022267533 2209 25 0.04231325897615079 2210 1 -2.2312513034591177 2210 2 -13.727789340887023 2210 3 -7.722792149220284 2210 4 -2.2807241625379326 2210 5 -0.9917642121700929 2210 6 -1.3059137724127972 2210 7 -1.3010370502291089 2210 8 -20.96902887870514 2210 9 -13.319069982579647 2210 10 -7.589457090750496 2210 11 -10.511455774176223 2210 12 -4.999169161613398 2210 13 -5.568320678864449 2210 14 -4.448543787367541 2210 15 -6.288832917337128 2210 16 -5.783811245762493 2210 17 -5.389824193808508 2210 18 -5.384537168113621 2210 19 -10.742873018698933 2210 20 -7.712468976504152 2210 21 -5.776795465127268 2210 22 -10.434303628748344 2210 23 -8.649385871790905 2210 24 -11.183391967290655 2210 25 -8.9646047261169 2211 1 0.5408163610377521 2211 2 4.93045035948751 2211 3 0.9649160199962381 2211 4 2.035212788966412 2211 5 1.3960386265324516 2211 6 0.3617583256564991 2211 7 -20.709708636944978 2211 8 -13.322961462974801 2211 9 -9.000424239942447 2211 10 -7.105651101005208 2211 11 -9.406303542878629 2211 12 -4.495410022898415 2211 13 -6.209637344840097 2211 14 -4.43891444950113 2211 15 -4.756275597381908 2211 16 -11.254660645267725 2211 17 -7.520692665414572 2211 18 -5.471971694144276 2211 19 -8.277375554735528 2211 20 -6.249117123884503 2211 21 -4.187861429719501 2211 22 -5.643799600112551 2211 23 -7.867277713630671 2211 24 -21.809775731128294 2211 25 -21.61681874825712 2212 1 -0.49692663878390064 2212 2 -13.727789340887023 2212 3 -7.532074349185141 2212 4 -19.682015675453062 2212 5 -19.940103695564925 2212 6 -20.81052631853826 2212 7 -9.705740382976723 2212 8 -9.083116180292441 2212 9 -4.572800287739842 2212 10 -5.1563243665661105 2212 11 -5.159335467258867 2212 12 -2.3562424644892843 2212 13 -5.870552272608958 2212 14 -3.47027034391882 2212 15 -3.2973514846656578 2212 16 -3.575843987518482 2212 17 -3.8140759363259313 2212 18 -2.616734936519876 2212 19 -3.34955096047835 2212 20 -3.307871805532443 2212 21 -1.2865550270086032 2212 22 -2.684574524314442 2212 23 -2.599153048034097 2212 24 -1.5984662073187685 2212 25 -2.1287235101618833 2213 1 -18.39176790862517 2213 2 -13.727789340887023 2213 3 -9.022006098690195 2213 4 -6.227967535409299 2213 5 -19.940103695564925 2213 6 -11.16829437105467 2213 7 -20.709708636944978 2213 8 -11.250210497961156 2213 9 -8.99690634518835 2213 10 -8.012472989399637 2213 11 -7.023529138892364 2213 12 -5.976021648956653 2213 13 -19.07293322498991 2213 14 -12.676504496952756 2213 15 -7.595923521065924 2213 16 -6.782814047606706 2213 17 -5.5847421167295295 2213 18 -5.6070899953924425 2213 19 -6.771065062107333 2213 20 -6.64325656118525 2213 21 -4.924849111965706 2213 22 -5.763084301675423 2213 23 -5.429503678439431 2213 24 -4.437784483863524 2213 25 -5.315943474734144 2214 1 -18.39176790862517 2214 2 -13.727789340887023 2214 3 -12.793392256629808 2214 4 -19.682015675453062 2214 5 -19.940103695564925 2214 6 -20.81052631853826 2214 7 -16.768394082954366 2214 8 -15.55823075663941 2214 9 -14.68755828013868 2214 10 -14.68955150718762 2214 11 -15.055601955074652 2214 12 -13.243049417317364 2214 13 -19.07293322498991 2214 14 -17.217769226100803 2214 15 -23.6495340243482 2214 16 -18.721691293577056 2214 17 -23.34185371410371 2214 18 -18.158024339605056 2214 19 -15.673983667833593 2214 20 -14.478939880649813 2214 21 -12.157978009347078 2214 22 -13.135306695117036 2214 23 -13.029988058094458 2214 24 -11.941231130189326 2214 25 -11.69663095482989 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 115036.0 3 7.0 4 628.0 5 2118.0 6 2793.0 7 2851.0 8 2451.0 9 2347.0 10 2215.0 11 2084.0 12 1945.0 13 2130.0 14 3059.0 15 6216.0 16 9189.0 17 9825.0 18 10233.0 19 12020.0 20 14119.0 21 16261.0 22 17239.0 23 18840.0 24 19610.0 25 20329.0 26 17238.0 27 13685.0 28 14536.0 29 17105.0 30 16288.0 31 18938.0 32 24249.0 33 29807.0 34 36127.0 35 41584.0 36 40259.0 37 42709.0 38 29103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21618027550121 18.298389777835595 12.155442811095211 26.329987135567983 2 14.039633733706392 20.11683904609335 41.77389626720411 24.069630952996153 3 19.21877498800576 23.953668905591982 28.95493496455035 27.872621141851912 4 11.296723664843451 16.402936526684957 38.67547382384474 33.624865984626844 5 12.846888651235295 37.46361181098081 36.67059399300332 13.018905544780585 6 32.14583636407162 37.41131062320572 17.9807640959254 12.462088916797256 7 28.097876365601905 31.063133859304457 22.304571236517226 18.534418538576414 8 24.448773576551847 38.26519491311841 20.661369534565317 16.62466197576443 9 26.18001399562479 15.381475340591724 20.054564023679085 38.3839466401044 10 13.775892945499121 29.80964754629526 35.37353835938722 21.0409211488184 11 33.6928377914552 22.035296195462013 25.289330831853217 18.98253518122957 12 23.974001361325023 24.52320258384828 32.046525284610354 19.456270770216342 13 28.88169606127242 21.31763377163458 25.376782001226644 24.423888165866355 14 19.618056943017024 22.768415853769643 28.075116320329645 29.538410882883685 15 22.10377136933829 32.50957621696914 23.15735769296178 22.22929472073079 16 20.912545064203833 28.403937294932557 29.143111759225505 21.540405881638108 17 20.747687460229823 28.83837885011763 29.560840075711088 20.85309361394146 18 20.552421595998187 27.49714110484319 31.745235707121367 20.20520159203726 19 22.73940415533336 27.088840363176647 27.571703124800468 22.600052356689527 20 22.963350090945838 27.99158119442199 28.586676434923202 20.458392279708974 21 21.600493421052633 26.506085526315786 27.107894736842102 24.785526315789475 22 21.936105822034428 29.02895749500802 28.04478955336745 20.990147129590106 23 20.91554567877885 28.669078071191596 28.834724225158986 21.580652024870563 24 22.229145881305083 27.298932912620355 29.320046812374205 21.15187439370035 25 21.63115928437796 28.003101806392433 28.814488966364 21.551249942865606 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 541.0 1 541.0 2 1208.0 3 1875.0 4 1875.0 5 1875.0 6 2733.0 7 3591.0 8 3591.0 9 3591.0 10 4192.5 11 4794.0 12 4794.0 13 4794.0 14 6042.0 15 7290.0 16 7290.0 17 7290.0 18 10237.5 19 13185.0 20 13185.0 21 13185.0 22 18937.0 23 24689.0 24 24689.0 25 24689.0 26 32629.0 27 40569.0 28 40569.0 29 40569.0 30 49875.5 31 59182.0 32 59182.0 33 59182.0 34 65552.5 35 71923.0 36 71923.0 37 71923.0 38 75630.5 39 79338.0 40 79338.0 41 79338.0 42 79819.0 43 80300.0 44 80300.0 45 80300.0 46 79065.0 47 77830.0 48 77830.0 49 77830.0 50 70963.5 51 64097.0 52 64097.0 53 64097.0 54 54643.0 55 45189.0 56 45189.0 57 45189.0 58 37280.0 59 29371.0 60 29371.0 61 29371.0 62 23185.5 63 17000.0 64 17000.0 65 17000.0 66 12834.5 67 8669.0 68 8669.0 69 8669.0 70 6160.0 71 3651.0 72 3651.0 73 3651.0 74 2542.5 75 1434.0 76 1434.0 77 1434.0 78 946.5 79 459.0 80 459.0 81 459.0 82 300.5 83 142.0 84 142.0 85 142.0 86 92.5 87 43.0 88 43.0 89 43.0 90 26.5 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.864054359993263 2 36.02199715668014 3 5.492204486021919 4 13.360611990748977 5 13.143348347615532 6 14.504079990805655 7 9.12428582449191 8 7.547077725281144 9 0.1084743841441623 10 1.3619911425706068 11 5.50952260250357 12 5.629489918494646 13 15.803725914042316 14 1.500378637001258 15 2.795773749828787 16 2.8335587312432993 17 2.764916015006935 18 1.4259107361301566 19 1.36986301369863 20 0.28795304586309556 21 4.278047083235591 22 1.5224198761597234 23 0.10831694672160185 24 8.79114823835396 25 0.11083594548256931 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 635173.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.46357871507787 #Duplication Level Percentage of deduplicated Percentage of total 1 97.41685662495877 91.04928047342288 2 1.8970826311003979 3.5461626364171814 3 0.29582870531254507 0.829476284554759 4 0.12570218573218064 0.4699430452334801 5 0.06737018342404698 0.3148329220751324 6 0.04220939446875897 0.2367024637467976 7 0.030106251423585625 0.19696865998209542 8 0.01818338016593605 0.13595870267561197 9 0.01282312499000069 0.10786456366885906 >10 0.08090209596195405 1.378479207753412 >50 0.007416343902213276 0.5163207632435014 >100 0.00482919374020504 0.8074395817689565 >500 6.898848193301696E-4 0.4105706954573729 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 774 0.12185656506180206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTAGG 55 2.3755924E-4 16.949984 1 TGTAGGA 465 0.0 16.430046 2 TAGGACC 235 0.0 16.376911 4 GTCCTAG 90 1.6514787E-7 16.277365 1 GTTCAGA 90 1.6514787E-7 16.277365 1 CTGTAGG 475 0.0 15.981412 1 AGGACTG 60 1.0951764E-5 15.589475 5 CTAGGAC 100 1.4424586E-9 15.260142 3 TCCTACA 695 0.0 15.115052 2 GTCCTAC 620 0.0 15.036275 1 TACTATA 55 0.003947319 14.883062 2 GGAGTAC 45 0.0046252143 14.533469 3 GTCTAGG 65 8.4600056E-4 14.342293 1 GTAGGAC 565 0.0 13.890395 3 GGACTGT 60 1.9143913E-4 13.852845 6 TCTTACA 70 0.0012344095 13.642807 2 TGGACTG 85 1.4336529E-6 13.449743 5 GTCTAAG 60 0.008246309 13.317843 1 GTAGTAG 60 0.008246309 13.317843 1 TCTAGGA 125 3.576297E-7 13.097095 2 >>END_MODULE