FastQCFastQC Report
Thu 26 May 2016
SRR1926459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635173
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17000.2676436183527952No Hit
CTGTAGGACGTGGAATATGGCAAGA16520.26008662206989275No Hit
GTCCTACAGTGTGCATTTCTCATTT14590.22970119951572246No Hit
CTTTAGGACGTGAAATATGGCGAGG12070.1900269690304846No Hit
GTCCTAAAGTGTGTATTTCTCATTT11960.18829515738231944No Hit
CCCATGTACTCTGCGTTGATACCAC10950.17239397770371223No Hit
CTGTAGGACCTGGAATATGGCGAGA10780.16971754152018428No Hit
GTACATGGGAAGCAGTGGTATCAAC9920.15617792317998402No Hit
GTATCAACGCAGAGTACTTTTTTTT6920.10894669641184371No Hit
CTGAAGGACCTGGAATATGGCGAGA6860.10800207187648088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA3050.013.4454534
TAGGACC3550.013.4322414
GTCCTAC9400.013.0857891
GTAGGAC8600.012.7558533
TAGCACC603.9545965E-412.7158564
TAGAACA2500.012.5886974
TGTAGGA9300.012.3115222
TCCTACA10200.012.2541892
GTAGCAC550.002971975412.1407493
CTGTAGG9300.012.0986561
GGCGAGG3500.011.90901119
ACGAAAT658.1509445E-411.66835712
CTTCATA907.139175E-611.6543541
GTAGAAC2300.011.612893
TACACTG3450.011.610135
AGGACGT7500.011.5714285
TTAGGAC5050.011.522553
GGACCTG6150.011.4752856
CCTACAG10250.011.4469923
GGACGTG7350.011.418326
Produced by FastQC (version 0.11.5)