FastQCFastQC Report
Thu 26 May 2016
SRR1926458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773788
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA405.5098947E-419.9726451
TCCAACG454.51529E-617.11569218
TAGGACC2400.016.5033254
CAATACT659.699397E-716.4690154
TTAGACT752.4959445E-715.7004614
GTCTTAG604.6198387E-415.5342791
CCAACGA350.00436341314.66316819
ACAATAC601.2612798E-414.55102353
GGACCGT652.3411743E-514.3872046
CTCCTAG751.5487452E-414.2027691
GTCCTAA1602.1827873E-1114.147291
TGAATAG450.006315302613.8677445
ATAGGAC806.8660556E-613.6415853
AGGACCT3250.013.4410465
GTAGGAC4800.013.4142263
TGTAGGA4900.013.3715132
GTATTGG1008.808438E-613.3150971
CTATAGG600.00826136613.3150961
CTATAAT802.6770428E-413.3150961
AGGACGG550.001551701613.2373935
Produced by FastQC (version 0.11.5)