FastQCFastQC Report
Thu 26 May 2016
SRR1926458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773788
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA13590.175629500586724No Hit
GTCCTACAGTGGACATTTCTAAATT13460.173949453855578No Hit
CCCATGTACTCTGCGTTGATACCAC12810.16554922019984802No Hit
GTACATGGGAAGCAGTGGTATCAAC11900.15378889308182603No Hit
GTCCTACAGTGTGCATTTCTCATTT10960.14164086287200112No Hit
CTTTAGGACGTGAAATATGGCGAGG10480.13543761340315436No Hit
GTCCTAAAGTGTGTATTTCTCATTT9650.12471116119660683No Hit
CTGTAGGACCTGGAATATGGCGAGA9120.11786173990808851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC1151.8189894E-1214.9268233
ATACCGT400.005151953514.301156
GGCGAAA400.005364504614.2142819
TAGGACC3450.013.5420674
TGGCGAA500.001526964213.26666218
CCTACAC1001.3684803E-712.3975543
TGTAGGA7800.012.3510582
GTAGGAC7250.012.1015923
CTATAGG1459.094947E-1111.8362051
CTACACT1459.094947E-1111.8362054
GTCCTAC7350.011.8048921
ACACGGT657.7779754E-411.7342786
GACGTGG3250.011.6924677
CTGTAGG7750.011.56469251
TATAGGA1501.6370905E-1011.446132
GGCGAGG2850.011.30492519
TAGGACG6350.011.2614814
TAGGACA3050.011.2540974
GGACCTG5200.011.1842346
GTCCTAA2900.011.17863751
Produced by FastQC (version 0.11.5)