##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926458_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 773788 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.181867643333835 31.0 28.0 31.0 18.0 34.0 2 28.678324037074756 31.0 28.0 33.0 22.0 34.0 3 29.057376180555913 31.0 28.0 33.0 24.0 34.0 4 32.87818239621188 35.0 33.0 37.0 27.0 37.0 5 32.57616944175924 35.0 33.0 37.0 26.0 37.0 6 32.672216421035216 35.0 33.0 37.0 26.0 37.0 7 32.49217098223286 35.0 33.0 37.0 25.0 37.0 8 32.45264982139811 35.0 33.0 37.0 25.0 37.0 9 33.67612059116968 37.0 33.0 39.0 22.0 39.0 10 33.57725888744721 37.0 33.0 39.0 22.0 39.0 11 33.83834848821641 37.0 34.0 39.0 25.0 39.0 12 33.64523357818938 37.0 33.0 39.0 23.0 39.0 13 33.76752288740585 37.0 34.0 39.0 25.0 39.0 14 34.411477045392274 38.0 33.0 40.0 19.0 41.0 15 34.5343427398719 38.0 34.0 40.0 23.0 41.0 16 34.502243508557896 38.0 34.0 40.0 23.0 41.0 17 34.42141387563519 38.0 34.0 40.0 22.0 41.0 18 34.38145848733762 38.0 34.0 40.0 20.0 41.0 19 34.39992220091291 39.0 34.0 40.0 19.0 41.0 20 34.38051119944998 39.0 34.0 40.0 19.0 41.0 21 34.306198855500476 39.0 34.0 40.0 18.0 41.0 22 34.23032019106008 39.0 34.0 40.0 18.0 41.0 23 33.87167544598779 39.0 34.0 40.0 11.0 41.0 24 33.962412185249704 39.0 34.0 40.0 16.0 41.0 25 33.74952312519708 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6617306501166667 1101 2 0.8155886917427395 1101 3 0.610297392090164 1101 4 0.5334804510268327 1101 5 0.5410491797870449 1101 6 0.1956315159225639 1101 7 0.2757117008997625 1101 8 0.7286241196454881 1101 9 0.8881587092878291 1101 10 0.8787248242582635 1101 11 0.6023790440915775 1101 12 0.6771674277855197 1101 13 0.6984653634483351 1101 14 0.9505502757974611 1101 15 0.7256694632676997 1101 16 0.7889462748635978 1101 17 0.7271012242227499 1101 18 0.8939296173611098 1101 19 1.0203693750357772 1101 20 0.8151424584764513 1101 21 1.046121582843341 1101 22 0.821615965784197 1101 23 1.2217496219792565 1101 24 1.1811613883844245 1101 25 1.0956108470560295 1102 1 1.041736518933174 1102 2 1.1110935313100363 1102 3 1.0322349620029918 1102 4 0.9831321830999116 1102 5 1.1325988562299258 1102 6 0.6693503598980897 1102 7 0.8560692574422788 1102 8 1.1100751293687523 1102 9 1.0253274024612438 1102 10 1.3173079904627087 1102 11 1.1435561830655914 1102 12 1.0123011198668124 1102 13 0.8450324367401407 1102 14 1.325163893658079 1102 15 1.1384568642865815 1102 16 1.0516800622579936 1102 17 1.0880157888161364 1102 18 1.1773228523366086 1102 19 1.1775935351441547 1102 20 1.0498603691777717 1102 21 1.3144053732162746 1102 22 1.2228223826510884 1102 23 1.5702712759264799 1102 24 1.5529073392319859 1102 25 1.584258217230527 1103 1 0.4977014346032753 1103 2 0.26752460048235704 1103 3 0.28080158828256785 1103 4 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2 0.34844159455214196 2203 3 0.308236358778192 2203 4 0.01639721427957852 2203 5 0.27964747714490557 2203 6 0.20629575543786416 2203 7 0.4239724696795051 2203 8 0.5260047051616681 2203 9 0.9270397197827549 2203 10 0.9622286036147543 2203 11 0.758697364539195 2203 12 0.7328919142884658 2203 13 0.6600536476704875 2203 14 1.1313866885222836 2203 15 1.1638339186812914 2203 16 0.9051961441883094 2203 17 0.8877727361017023 2203 18 0.9646056290150753 2203 19 1.0587322296203254 2203 20 0.9911393404505304 2203 21 1.2488762178388626 2203 22 1.1090197774118025 2203 23 1.4380524432018333 2203 24 1.1842191900397978 2203 25 1.1769363262366141 2204 1 0.7061462630340536 2204 2 0.9041391329296502 2204 3 0.8276585973340573 2204 4 0.7303492180917601 2204 5 0.5629874975319922 2204 6 0.5236121901930062 2204 7 0.938421563218462 2204 8 0.9882885981283493 2204 9 1.2854899641692867 2204 10 1.1548281142797592 2204 11 1.0607341624406956 2204 12 1.0756815283386985 2204 13 0.9166156703750019 2204 14 1.248685478446518 2204 15 1.2685520565559827 2204 16 1.3607868020932514 2204 17 1.3625136549141246 2204 18 1.208511700073167 2204 19 1.2643276639922263 2204 20 1.3425996468861783 2204 21 1.288033694021152 2204 22 1.111792376000885 2204 23 1.2563151803534183 2204 24 1.3084843165487428 2204 25 1.2167355348041369 2205 1 0.6930723069995857 2205 2 0.7833738616853836 2205 3 0.7604211091568054 2205 4 0.6029871397437532 2205 5 0.6068390964253112 2205 6 0.6330271033471888 2205 7 0.40559568458930784 2205 8 0.4547296831262315 2205 9 1.03290872927613 2205 10 1.0794252564263829 2205 11 0.8284667233024834 2205 12 0.9981227423593069 2205 13 0.7027386519237098 2205 14 0.9049580119013854 2205 15 0.9202692553893641 2205 16 0.6982993271824469 2205 17 1.052014222510067 2205 18 0.9715536605526722 2205 19 0.9171108610713219 2205 20 0.8596795504520927 2205 21 0.9390568754510369 2205 22 1.0218187944444779 2205 23 1.2968761794887556 2205 24 0.9991237178000532 2205 25 1.2679289429914604 2206 1 0.40351140295936716 2206 2 0.6838853410128394 2206 3 0.7594477337183818 2206 4 0.6566304901060747 2206 5 0.8888040142675493 2206 6 0.7032645744405812 2206 7 0.6758831453189913 2206 8 0.5192224102437777 2206 9 0.9945487957664056 2206 10 1.0111467288040643 2206 11 0.7701109352747935 2206 12 0.6848320018217748 2206 13 0.6090558381981026 2206 14 0.8364325842461398 2206 15 0.7667909114687959 2206 16 0.9091590676427614 2206 17 0.7240253096424141 2206 18 0.7569883874995256 2206 19 0.7346812021794662 2206 20 0.7862679406638762 2206 21 0.8635837011904144 2206 22 0.8323438481934957 2206 23 1.0484205747564062 2206 24 1.0757419798676722 2206 25 1.1350682758939854 2207 1 -0.9297614659250115 2207 2 0.05682083876848054 2207 3 0.4217158679289632 2207 4 0.5304032667565508 2207 5 0.4728301267109245 2207 6 0.3692282137855116 2207 7 0.5279686563973129 2207 8 0.36243706928577524 2207 9 0.6193433191220805 2207 10 0.42056073605979805 2207 11 0.20802726887458078 2207 12 0.39679986342992635 2207 13 0.4266120496392034 2207 14 -4.543777094164973 2207 15 -1.745988608504966 2207 16 -0.8076592725568759 2207 17 -0.44512347389485996 2207 18 -0.20434715944752924 2207 19 -0.044912558709263806 2207 20 0.33911796833405106 2207 21 0.3619245157182789 2207 22 0.370118756472003 2207 23 0.49902726888827686 2207 24 0.1239926101326887 2207 25 0.08751042473901549 2208 1 0.6434625175622912 2208 2 0.8832401490027451 2208 3 0.8312898243626989 2208 4 0.5712040523119484 2208 5 0.7335373269674363 2208 6 0.6298432315206313 2208 7 0.7372259859097454 2208 8 0.9981227268878285 2208 9 1.1822298344223796 2208 10 1.1639155786481226 2208 11 0.8808093774495589 2208 12 0.8410033864054185 2208 13 0.6393532772699118 2208 14 1.0069323104561718 2208 15 0.7683046922304868 2208 16 0.8485780773960272 2208 17 0.818421344846243 2208 18 1.0208266847640104 2208 19 0.655111488905014 2208 20 0.6736152729972815 2208 21 0.7263871517375975 2208 22 0.5020499802951761 2208 23 0.8900945821685724 2208 24 0.7217732157317798 2208 25 0.6069829803992235 2209 1 -0.2960736335821892 2209 2 -0.09109749568701986 2209 3 0.49936652559000905 2209 4 0.6452911648389659 2209 5 0.506417759184167 2209 6 0.537005208107523 2209 7 0.774143301058885 2209 8 0.5539741564914706 2209 9 0.8348912639644368 2209 10 0.5159902435959935 2209 11 0.6891589442270032 2209 12 0.6395040935609302 2209 13 0.7299605278205519 2209 14 0.7883170831092698 2209 15 0.8472264288976774 2209 16 0.8846963786627526 2209 17 0.9504444048898009 2209 18 0.8356781821067898 2209 19 0.8544379976916048 2209 20 0.6545010340259338 2209 21 0.541265847369246 2209 22 0.6972156640601526 2209 23 0.9116238239213317 2209 24 0.6698157600136696 2209 25 0.8519882455774024 2210 1 -0.3537982488634235 2210 2 -0.3288195824339688 2210 3 0.40091371521527464 2210 4 0.4437721080050352 2210 5 0.9812964294029314 2210 6 0.7604427846327013 2210 7 0.7810870890082526 2210 8 0.7688095910519763 2210 9 1.0949618351284442 2210 10 1.0597999474727402 2210 11 0.7861965726896472 2210 12 0.6035715712568646 2210 13 0.5981453021716447 2210 14 1.150957875201108 2210 15 0.8878999529796943 2210 16 0.9498124007999564 2210 17 0.8347092904964057 2210 18 0.726175563023645 2210 19 1.0315521427907441 2210 20 0.8942812565597293 2210 21 0.8927463497502828 2210 22 1.0073153361479825 2210 23 1.1669767423852164 2210 24 1.0988994852508327 2210 25 1.0994315215555304 2211 1 -1.4455012029025518 2211 2 -0.26989641756344085 2211 3 0.18974473250852952 2211 4 0.2986708444751969 2211 5 0.38030579001589615 2211 6 0.24414581395195256 2211 7 0.3992957043479919 2211 8 0.4401918517611634 2211 9 0.8028655424393207 2211 10 0.7430282415074956 2211 11 0.35585421279889573 2211 12 0.6410822457432559 2211 13 0.6147245733188882 2211 14 0.694323931863515 2211 15 0.4052602554175877 2211 16 0.5818066889205156 2211 17 0.5191702965682197 2211 18 0.4875234536510291 2211 19 0.4237886447115642 2211 20 0.581959311697716 2211 21 0.6775886967022586 2211 22 0.5233441213855485 2211 23 0.5872821444398895 2211 24 0.8848392752663372 2211 25 0.6801059373700014 2212 1 0.5359092647386738 2212 2 -0.10158033878273898 2212 3 0.12310679325372931 2212 4 0.0885334477822255 2212 5 0.09339619566384982 2212 6 0.028946719710354785 2212 7 0.414244821825001 2212 8 0.3146669428017148 2212 9 0.5994772769835492 2212 10 0.2196673033040213 2212 11 0.177136517558111 2212 12 0.24605619191194705 2212 13 0.24311317070903726 2212 14 0.2997112872155654 2212 15 0.08846389293559298 2212 16 -0.43676715199480043 2212 17 0.055978198296330106 2212 18 0.26941238961425285 2212 19 0.12825479162934528 2212 20 0.2934051333680827 2212 21 0.25268553101389557 2212 22 0.20945490056228522 2212 23 0.6403596589635328 2212 24 0.4040969010060991 2212 25 -0.03634183366045107 2213 1 0.3424615182526374 2213 2 0.24919144834667506 2213 3 -0.418555692985219 2213 4 -1.319103609544836 2213 5 -2.076838838290385 2213 6 -0.4372179291268452 2213 7 -0.2321562765852434 2213 8 -0.21505005713094505 2213 9 -0.6756606084118033 2213 10 -0.5271444620001233 2213 11 -0.3643328672799271 2213 12 -0.6486148966164578 2213 13 -0.5920593217558832 2213 14 -7.160030850258888 2213 15 -3.176814138722044 2213 16 -1.980935833284029 2213 17 -1.303654950273554 2213 18 -5.310113776656362 2213 19 -3.122513147215926 2213 20 -5.3260504168884495 2213 21 -2.274480606284637 2213 22 -1.7712225898616722 2213 23 -4.47511085562078 2213 24 -2.070142272602368 2213 25 -1.412737695655487 2214 1 0.13875254050511288 2214 2 -4.534219832379449E-4 2214 3 -1.695433538798163 2214 4 -2.078058260614739 2214 5 -0.5650110232148258 2214 6 -0.6795066660460911 2214 7 -0.7013042779606131 2214 8 -0.9036404094126276 2214 9 -6.536045155669228 2214 10 -2.302000934042997 2214 11 -1.4248716224332156 2214 12 -2.013139439636639 2214 13 -1.695863116520453 2214 14 -3.524541500675838 2214 15 -2.81952416883426 2214 16 -2.4454975390178575 2214 17 -2.7242587228099566 2214 18 -2.3386387642398496 2214 19 -4.984181023696788 2214 20 -2.8065330459246844 2214 21 -4.7795844013965265 2214 22 -6.592799886569477 2214 23 -6.897515456850968 2214 24 -6.783994587283722 2214 25 -4.590439720155093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26117.0 3 17.0 4 475.0 5 3079.0 6 3767.0 7 3123.0 8 2439.0 9 1908.0 10 1718.0 11 1597.0 12 1468.0 13 1564.0 14 1472.0 15 1551.0 16 1597.0 17 1766.0 18 1922.0 19 2192.0 20 2870.0 21 3729.0 22 4712.0 23 5880.0 24 7186.0 25 8343.0 26 9558.0 27 10666.0 28 12211.0 29 14540.0 30 18266.0 31 22748.0 32 28900.0 33 37385.0 34 49155.0 35 64749.0 36 86639.0 37 127443.0 38 201036.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.562266376596405 18.918289689140213 8.879047424419191 30.640396509844198 2 14.16720960536113 20.788476255481093 38.33185964259121 26.712454496566558 3 18.877451430299942 23.717810333527666 28.524688493353622 28.88004974281877 4 12.438174329645554 16.76732863979647 36.52943716340075 34.26505986715722 5 13.524441824631072 38.06606066246807 34.95279165244728 13.456705860453571 6 33.31339961187618 37.16441298865723 16.77818308236735 12.744004317099241 7 29.267926345924515 31.023950134207247 20.982166556823465 18.725956963044776 8 25.920108562645595 37.5627530678918 19.225196271266768 17.291942098195847 9 26.53763128963293 15.364982265944787 19.02717149912657 39.07021494529572 10 14.783602268673384 29.499216718282263 33.718856150183285 21.99832486286107 11 35.37714146876066 22.40775012665295 23.466853113594773 18.748255290991615 12 23.783762127484138 24.501815164670138 30.961966193608088 20.75245651423764 13 29.529664455897482 21.650116052458813 25.087103961291724 23.733115530351984 14 20.950331515504995 22.00559444538562 26.77884870297064 30.265225336138744 15 23.18224172847452 30.96272918661771 22.453212780558538 23.401816304349232 16 22.53201855848637 28.047223335099574 27.157940990216733 22.26281711619732 17 21.970722074242946 28.69169519629548 27.2549392121816 22.082643517279973 18 22.032040777291268 27.499599361045483 28.922643079802114 21.545716781861135 19 23.798200256663097 26.843392863835447 26.559460929957858 22.798945949543604 20 23.844565237057658 27.265830767998057 27.385761807650255 21.50384218729403 21 22.92827354297759 26.60241125787846 26.549166631127168 23.92014856801678 22 23.423082291273577 28.02305024115132 26.22669258246445 22.32717488511065 23 22.4663602950679 27.73524531266962 26.931924506453964 22.866469885808517 24 23.54869059065579 26.794557654332472 27.461281184413224 22.195470570598513 25 22.888052024585544 27.303473302765095 27.130557723820996 22.67791694882836 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 90.0 1 90.0 2 96.5 3 103.0 4 103.0 5 103.0 6 201.0 7 299.0 8 299.0 9 299.0 10 507.5 11 716.0 12 716.0 13 716.0 14 1342.0 15 1968.0 16 1968.0 17 1968.0 18 3774.0 19 5580.0 20 5580.0 21 5580.0 22 9634.0 23 13688.0 24 13688.0 25 13688.0 26 20884.5 27 28081.0 28 28081.0 29 28081.0 30 39029.0 31 49977.0 32 49977.0 33 49977.0 34 59708.5 35 69440.0 36 69440.0 37 69440.0 38 77248.0 39 85056.0 40 85056.0 41 85056.0 42 91481.5 43 97907.0 44 97907.0 45 97907.0 46 103102.5 47 108298.0 48 108298.0 49 108298.0 50 104905.5 51 101513.0 52 101513.0 53 101513.0 54 90885.0 55 80257.0 56 80257.0 57 80257.0 58 69140.5 59 58024.0 60 58024.0 61 58024.0 62 47626.5 63 37229.0 64 37229.0 65 37229.0 66 28891.0 67 20553.0 68 20553.0 69 20553.0 70 14928.0 71 9303.0 72 9303.0 73 9303.0 74 6504.5 75 3706.0 76 3706.0 77 3706.0 78 2566.5 79 1427.0 80 1427.0 81 1427.0 82 912.0 83 397.0 84 397.0 85 397.0 86 271.0 87 145.0 88 145.0 89 145.0 90 87.0 91 29.0 92 29.0 93 29.0 94 15.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08296846164582547 2 0.02843156006554767 3 0.027009982062270288 4 0.031274716072102436 5 0.025329935331124286 6 0.374779655409492 7 0.1906206868031037 8 0.19579006136047603 9 0.1996670922785052 10 0.015508123672116911 11 0.002584687278686152 12 0.0037477965540949204 13 0.0 14 0.06733110360977425 15 0.002455452914751844 16 0.0023262185508175367 17 0.004006265281963535 18 0.0031016247344233822 19 0.0014215780032773835 20 0.001292343639343076 21 1.292343639343076E-4 22 0.0 23 0.0 24 5.169374557372304E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 773788.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.51260287605606 #Duplication Level Percentage of deduplicated Percentage of total 1 90.93652317564795 75.03409223721508 2 6.758233973072258 11.152789519271636 3 1.1941636618049034 2.956006559865747 4 0.39426852457921446 1.3012848878053307 5 0.20931142142126763 0.8635415096577934 6 0.12057970876390607 0.5969607374488006 7 0.07343915172945269 0.4241758893544729 8 0.05227916679762688 0.3450952102930946 9 0.03522049602566697 0.26155213214972123 >10 0.19797778383252623 2.9142763523805293 >50 0.014631443533559926 0.851697551761766 >100 0.011798376021007698 1.940279709896096 >500 6.292467082633685E-4 0.4080973602750065 >1k 9.438700623950527E-4 0.9501503426250151 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1359 0.175629500586724 No Hit GTCCTACAGTGGACATTTCTAAATT 1346 0.173949453855578 No Hit CCCATGTACTCTGCGTTGATACCAC 1281 0.16554922019984802 No Hit GTACATGGGAAGCAGTGGTATCAAC 1190 0.15378889308182603 No Hit GTCCTACAGTGTGCATTTCTCATTT 1096 0.14164086287200112 No Hit CTTTAGGACGTGAAATATGGCGAGG 1048 0.13543761340315436 No Hit GTCCTAAAGTGTGTATTTCTCATTT 965 0.12471116119660683 No Hit CTGTAGGACCTGGAATATGGCGAGA 912 0.11786173990808851 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGGAC 115 1.8189894E-12 14.926823 3 ATACCGT 40 0.0051519535 14.30115 6 GGCGAAA 40 0.0053645046 14.21428 19 TAGGACC 345 0.0 13.542067 4 TGGCGAA 50 0.0015269642 13.266662 18 CCTACAC 100 1.3684803E-7 12.397554 3 TGTAGGA 780 0.0 12.351058 2 GTAGGAC 725 0.0 12.101592 3 CTATAGG 145 9.094947E-11 11.836205 1 CTACACT 145 9.094947E-11 11.836205 4 GTCCTAC 735 0.0 11.804892 1 ACACGGT 65 7.7779754E-4 11.734278 6 GACGTGG 325 0.0 11.692467 7 CTGTAGG 775 0.0 11.5646925 1 TATAGGA 150 1.6370905E-10 11.44613 2 GGCGAGG 285 0.0 11.304925 19 TAGGACG 635 0.0 11.261481 4 TAGGACA 305 0.0 11.254097 4 GGACCTG 520 0.0 11.184234 6 GTCCTAA 290 0.0 11.1786375 1 >>END_MODULE