FastQCFastQC Report
Thu 26 May 2016
SRR1926457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890002
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9810.11022447140568224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA6900.015.31732
GTCCTAC7600.014.7819611
TAGGACC3050.014.7816524
ACGTGCA400.00439631314.6465918
CTGTAGG6950.013.855241
GTAGGAC6900.013.6677153
AACTGCG450.007193887613.59802157
CTAGGAC1054.1676685E-813.57690053
TCCTACA8750.013.4905922
GCCATAT2100.013.3741561
AGGACGT5200.012.8448555
GTTCTAA957.724804E-512.6702521
CTACACT1202.3266693E-812.5233454
GTCCTAA3100.012.5113071
GTCTAGG750.00241226112.4825451
GTCTTAA750.00241226112.4825451
TAGGACA2600.012.3857264
CCTTATA905.5989216E-412.2007932
ATAGGAC902.1974089E-512.1843993
TACACTT600.00290850412.1758525
Produced by FastQC (version 0.11.5)