##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926457_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 890002 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.181006334817226 27.0 10.0 30.0 2.0 31.0 2 16.46853714935472 18.0 2.0 30.0 2.0 31.0 3 20.245625290729684 25.0 15.0 31.0 2.0 31.0 4 22.568104341338557 28.0 7.0 35.0 2.0 37.0 5 22.91169795124056 30.0 7.0 35.0 2.0 37.0 6 23.801962242781478 31.0 2.0 35.0 2.0 37.0 7 23.71091300918425 31.0 7.0 35.0 2.0 37.0 8 23.90111370536246 31.0 10.0 35.0 2.0 37.0 9 26.601473929272068 32.0 17.0 37.0 2.0 39.0 10 27.262346601468312 32.0 24.0 37.0 2.0 39.0 11 24.34408574362754 30.0 8.0 37.0 2.0 39.0 12 25.490951705726506 31.0 16.0 37.0 2.0 39.0 13 22.082425657470434 28.0 2.0 37.0 2.0 39.0 14 25.526069604337966 31.0 17.0 38.0 2.0 40.0 15 26.631044649337866 31.0 17.0 38.0 2.0 40.0 16 27.007337062163906 32.0 18.0 39.0 2.0 40.0 17 26.316667827712745 31.0 17.0 38.0 2.0 40.0 18 26.40464740528673 31.0 17.0 38.0 2.0 40.0 19 26.79793079116676 31.0 18.0 39.0 2.0 40.0 20 27.034060597616634 31.0 19.0 39.0 2.0 40.0 21 24.853774485900033 30.0 8.0 38.0 2.0 40.0 22 25.801416176592863 31.0 10.0 38.0 2.0 40.0 23 25.811691434401272 31.0 2.0 38.0 2.0 40.0 24 24.853195835514978 31.0 2.0 39.0 2.0 40.0 25 24.659664809742 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.703071979136098 1101 2 10.61345754644163 1101 3 7.419871809408047 1101 4 8.95481965936775 1101 5 8.459120538283461 1101 6 8.13465561465685 1101 7 7.79779604614971 1101 8 6.114908698739892 1101 9 6.251386438227506 1101 10 5.590309037069456 1101 11 8.557701451641062 1101 12 6.321925509979696 1101 13 10.752377106521081 1101 14 8.036839597916686 1101 15 7.20407739283079 1101 16 6.73072676098791 1101 17 2.560945962235003 1101 18 5.102774869067773 1101 19 5.629341117655123 1101 20 3.8972301155648275 1101 21 0.7601036933492047 1101 22 4.88987959454305 1101 23 5.955139269121467 1101 24 7.222300319293584 1101 25 7.647083597867237 1102 1 5.885188393423601 1102 2 11.062980569566896 1102 3 7.4892162816434755 1102 4 8.705967682707602 1102 5 8.527849584175211 1102 6 7.555163474432341 1102 7 7.407993840914894 1102 8 7.449343991423614 1102 9 6.438103958394095 1102 10 5.704998929142388 1102 11 8.564040686315057 1102 12 5.996005331720639 1102 13 10.57389145016073 1102 14 7.9781054363470965 1102 15 5.983235039824983 1102 16 6.453240721810058 1102 17 7.019746730616678 1102 18 7.033937243772293 1102 19 5.740187813837075 1102 20 5.9715349069636225 1102 21 4.989422964862946 1102 22 6.246224751729976 1102 23 6.520376607389657 1102 24 8.009318170949225 1102 25 7.846913475829137 1103 1 4.961860647342167 1103 2 9.564600343856021 1103 3 6.679630972062132 1103 4 8.220354415716276 1103 5 7.594016658869897 1103 6 7.265157781428041 1103 7 7.152708864629872 1103 8 6.74426761175134 1103 9 5.581683553820657 1103 10 4.860843600014476 1103 11 5.962842247220507 1103 12 4.752348272659685 1103 13 8.943764000891036 1103 14 6.9085309311361485 1103 15 5.918048818657937 1103 16 5.777653840043005 1103 17 3.6293264881969236 1103 18 2.479009446224687 1103 19 4.355579106536812 1103 20 4.81331124768376 1103 21 6.974498216509598 1103 22 2.610615362326719 1103 23 4.903130241306567 1103 24 6.166244160130756 1103 25 2.0527420194599344 1104 1 5.46761443186087 1104 2 10.489491820479607 1104 3 7.051641789982266 1104 4 7.496021325253647 1104 5 7.553520457999806 1104 6 7.3641732675742375 1104 7 7.218764501503099 1104 8 7.2927618783142805 1104 9 6.1917505229284515 1104 10 5.025015105695964 1104 11 8.08513219328243 1104 12 5.146033461139908 1104 13 9.562502882845916 1104 14 7.122394822236561 1104 15 6.451146554418941 1104 16 5.943793853922568 1104 17 6.22319168672966 1104 18 3.8006468883156117 1104 19 4.9833743383917515 1104 20 3.9284964679839405 1104 21 3.2390134210383366 1104 22 5.380812052964423 1104 23 6.0186704597863 1104 24 6.892771745437468 1104 25 5.914847305859045 1105 1 2.6822966267248844 1105 2 9.091826674841755 1105 3 6.035127329593191 1105 4 5.105662577242477 1105 5 1.4305917448001075 1105 6 4.244884518458793 1105 7 5.61066881772048 1105 8 3.040331340996687 1105 9 3.763134308979133 1105 10 3.339356464927455 1105 11 6.493328874591395 1105 12 3.7877792707972056 1105 13 4.903698334565831 1105 14 3.474017999721312 1105 15 3.999315798480165 1105 16 4.155623137497731 1105 17 4.903144918505745 1105 18 1.9273330238202107 1105 19 1.82357615146692 1105 20 0.9982000638184765 1105 21 1.9782201892787903 1105 22 3.70260704618369 1105 23 0.7491042569831023 1105 24 4.111698566070906 1105 25 5.048358012134496 1106 1 6.364284724636953 1106 2 11.12030557048887 1106 3 7.877768882784043 1106 4 9.186368188284707 1106 5 8.543765372731333 1106 6 7.791939850436876 1106 7 7.559815326600827 1106 8 7.273190769375358 1106 9 6.14055691256695 1106 10 5.521143870186332 1106 11 7.921482526508434 1106 12 5.2567144807217225 1106 13 4.439786117126896 1106 14 5.76814342168268 1106 15 5.998912791414973 1106 16 5.974925554161626 1106 17 6.48071202080386 1106 18 6.386681206517828 1106 19 6.290513354533019 1106 20 5.847042411833318 1106 21 6.1073543565745645 1106 22 6.4850537774882575 1106 23 6.82924549595818 1106 24 7.39615506198529 1106 25 6.691651350192554 1107 1 3.654807834748045 1107 2 -13.933061646791941 1107 3 -5.726237019168018 1107 4 1.4464005466010512 1107 5 4.197220127099385 1107 6 4.3992896051089865 1107 7 4.677337466922619 1107 8 4.85932518281286 1107 9 3.153681874559531 1107 10 2.6437646057539155 1107 11 5.339513726198614 1107 12 2.3742478232301814 1107 13 4.399546262307236 1107 14 3.4953405705661424 1107 15 3.250636327849282 1107 16 3.0780072757136736 1107 17 3.659613342341821 1107 18 -1.172766397654506 1107 19 1.3398717892994831 1107 20 1.5140902195653894 1107 21 -1.6976163737119876 1107 22 1.4650210671031907 1107 23 -1.354531177248429 1107 24 1.965101320294913 1107 25 2.7861609984759 1108 1 5.200406071523865 1108 2 10.81131335320806 1108 3 0.35151014707883377 1108 4 6.0953064030242246 1108 5 7.571883225336162 1108 6 7.430612023245008 1108 7 7.623308814529672 1108 8 7.188026379286409 1108 9 5.58189188715399 1108 10 4.819303018852153 1108 11 7.332807177080227 1108 12 2.535853616681063 1108 13 3.659097584725373 1108 14 5.166916452178235 1108 15 5.151821781249787 1108 16 4.995105827350951 1108 17 0.09055477811008572 1108 18 3.6553208189701785 1108 19 4.814259383757921 1108 20 5.187268746013753 1108 21 5.232638664071388 1108 22 2.0282184852392113 1108 23 4.745952135094143 1108 24 5.4970770759624195 1108 25 6.090317169760535 1109 1 3.5011419389702603 1109 2 -13.933061646791941 1109 3 -5.701891006267497 1109 4 2.0334582061431306 1109 5 1.4835214644524726 1109 6 2.6443254735374033 1109 7 3.5419057118656028 1109 8 3.826502256479394 1109 9 3.274990896250806 1109 10 2.441673876551956 1109 11 0.6689913876065425 1109 12 1.2858844808785932 1109 13 2.642354163672742 1109 14 3.157050061664915 1109 15 3.148462863121132 1109 16 2.9461832315094973 1109 17 3.558529924308914 1109 18 3.649843642232039 1109 19 2.0537017976630523 1109 20 1.1357494965010808 1109 21 0.2485069226808747 1109 22 2.5386794176628626 1109 23 2.862444581487903 1109 24 4.090296292986462 1109 25 4.36663295923422 1110 1 3.175974768866702 1110 2 9.195259804925492 1110 3 6.205238679159194 1110 4 7.42537971799506 1110 5 7.952094664091835 1110 6 3.932546566342868 1110 7 6.338012719260714 1110 8 7.003066479740074 1110 9 5.197583316188339 1110 10 4.932338501677815 1110 11 2.901723249666098 1110 12 5.672929118949369 1110 13 10.010979875716949 1110 14 7.414034485230726 1110 15 6.791392827912134 1110 16 6.329406297214856 1110 17 6.7351813173194195 1110 18 6.992480977751775 1110 19 5.891993910653575 1110 20 5.960900874983292 1110 21 3.9907956634233024 1110 22 5.836418015375308 1110 23 6.4580742186975115 1110 24 7.512344007265078 1110 25 7.776156443901819 1111 1 5.05592524085294 1111 2 9.54425464713777 1111 3 7.394631153468609 1111 4 8.068139869477896 1111 5 8.22381213907418 1111 6 7.44733726286162 1111 7 7.105519277788456 1111 8 7.323851059797594 1111 9 5.88945778172268 1111 10 5.048779855912855 1111 11 6.953773630754348 1111 12 6.163114000067644 1111 13 7.933492552776489 1111 14 5.6873537524977245 1111 15 5.4237127556906835 1111 16 5.068250795402072 1111 17 6.366022030506247 1111 18 6.297754924401488 1111 19 5.235267770339391 1111 20 4.88645604633324 1111 21 7.396612306243913 1111 22 6.738468085878189 1111 23 6.97929279004622 1111 24 6.785138178198842 1111 25 7.221786818322837 1112 1 -2.0561348491922473 1112 2 7.51003298236407 1112 3 5.540520696950956 1112 4 -0.5372943003005837 1112 5 3.7017457573054706 1112 6 2.695177240636312 1112 7 5.242485604811002 1112 8 5.538453169567742 1112 9 4.076142206847084 1112 10 2.807624630105348 1112 11 -0.55916852624458 1112 12 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6.117345337450452 2204 5 1.2326824056640326 2204 6 4.296431695376153 2204 7 5.526249388300162 2204 8 5.648118592401165 2204 9 4.136000493711364 2204 10 3.807684166393134 2204 11 5.338913734457275 2204 12 5.2031077150417175 2204 13 2.9746406175122573 2204 14 4.3758406325061046 2204 15 4.657549240266182 2204 16 4.420874269973904 2204 17 -1.520818172709589 2204 18 2.6643269665111617 2204 19 3.6309499575284114 2204 20 4.017565877161292 2204 21 5.457208336202537 2204 22 5.021671354091669 2204 23 4.0134623809957795 2204 24 5.605857813667338 2204 25 5.453431923858897 2205 1 6.053051457111728 2205 2 10.520162120591117 2205 3 7.437670861364548 2205 4 7.927358394174668 2205 5 7.915152820784961 2205 6 7.460251719831607 2205 7 7.651245761432325 2205 8 7.29487451455822 2205 9 5.925130667179275 2205 10 5.200382348814227 2205 11 7.5221086941472315 2205 12 6.814017333495222 2205 13 9.933591105585045 2205 14 7.366857191748398 2205 15 6.4478876788477635 2205 16 6.033330384873079 2205 17 6.689794664330062 2205 18 6.5633462614480536 2205 19 5.036452019661841 2205 20 5.33612383450933 2205 21 -1.012852802426714 2205 22 3.6154269239244208 2205 23 5.00774021979705 2205 24 7.032054161929551 2205 25 7.111492896688475 2206 1 3.8658160393695233 2206 2 8.539607163497447 2206 3 5.853610227464685 2206 4 1.3234514994872448 2206 5 5.601871167458349 2206 6 5.909856396235362 2206 7 6.028447480124527 2206 8 5.953032408225319 2206 9 4.653280552748843 2206 10 3.820054626569192 2206 11 3.4943425468551474 2206 12 4.623401848192316 2206 13 7.969143806590324 2206 14 0.13396830105283186 2206 15 2.4688253986131308 2206 16 3.339271824135519 2206 17 4.421740469428411 2206 18 -0.9863250813513638 2206 19 1.1964277921180475 2206 20 2.569726543441405 2206 21 3.154877410052098 2206 22 -1.8480455822849038 2206 23 -2.9033264018833513 2206 24 1.26706702773091 2206 25 2.9155583273168055 2207 1 1.3359705876528984 2207 2 -13.933061646791941 2207 3 -6.331453562598586 2207 4 -4.297979887298357 2207 5 1.3924074188845488 2207 6 1.730773313567589 2207 7 2.8359902661425735 2207 8 3.000344927673506 2207 9 0.5737265645733451 2207 10 0.2807143091747335 2207 11 -1.665337984210094 2207 12 -2.5155173510608755 2207 13 0.20778710085440366 2207 14 -5.696047257499185 2207 15 -1.6242064445566626 2207 16 -0.4110030436167875 2207 17 0.6740628426262134 2207 18 0.4276796899379214 2207 19 -0.11414787430659601 2207 20 -0.23759012495398935 2207 21 -3.7690952068963526 2207 22 -4.959301676051112 2207 23 -1.9250357681316643 2207 24 -0.22770114984403023 2207 25 0.4699945891153732 2208 1 4.947466986799064 2208 2 6.676091105217953 2208 3 6.382321448872279 2208 4 8.306699028256133 2208 5 3.6744087355402435 2208 6 6.320973804321074 2208 7 6.874104655593371 2208 8 6.687388237666127 2208 9 5.067150467858998 2208 10 4.521057038641885 2208 11 6.751893447952213 2208 12 6.4142657997361 2208 13 9.05168918954912 2208 14 3.38820394444787 2208 15 4.898801914212065 2208 16 5.247424550294674 2208 17 6.303227165246756 2208 18 0.8582193192793923 2208 19 3.5777910009502527 2208 20 4.65125181651468 2208 21 7.1670936733478285 2208 22 6.3630503498032205 2208 23 2.1973872773328544 2208 24 3.974824834156607 2208 25 5.164374065246001 2209 1 3.276836971132731 2209 2 4.850510713051605 2209 3 5.377231300924986 2209 4 0.8159428111076643 2209 5 5.367966993263149 2209 6 5.587020106686989 2209 7 6.1763637363028145 2209 8 4.833478628308576 2209 9 4.705760042303925 2209 10 3.672528247013819 2209 11 -0.2955663561661872 2209 12 3.3712740860422095 2209 13 2.5493053748166368 2209 14 4.098765376558937 2209 15 4.458892348394507 2209 16 3.967766355382242 2209 17 2.089679615137463 2209 18 3.8805400171448845 2209 19 4.242029103445013 2209 20 4.338154502206713 2209 21 6.658096617135275 2209 22 5.291930186673369 2209 23 1.1546084258372957 2209 24 -2.39912647684071 2209 25 0.7386222545062964 2210 1 -2.096663464899976 2210 2 -13.933061646791941 2210 3 -7.7070784290801075 2210 4 -2.0208980671744534 2210 5 -0.7842343243327115 2210 6 -1.42207009596029 2210 7 -1.1303459867948327 2210 8 -21.31384862071359 2210 9 -13.282334106223495 2210 10 -7.070539696379637 2210 11 -9.72985838583368 2210 12 -4.393103336961321 2210 13 -5.396370130506416 2210 14 -3.9883608657025604 2210 15 -5.891965470405843 2210 16 -5.229823808410636 2210 17 -4.693559460300513 2210 18 -4.861579015466905 2210 19 -10.222466609619563 2210 20 -7.1548214857743275 2210 21 -5.43679014387563 2210 22 -10.114947905489265 2210 23 -8.189979156296587 2210 24 -10.856784679004468 2210 25 -8.606702697789133 2211 1 -0.42573516006917345 2211 2 4.358103011842598 2211 3 0.006517677199315841 2211 4 0.6947583775891495 2211 5 0.2168915921366974 2211 6 -0.871844469392208 2211 7 -21.10981618547033 2211 8 -14.025361338250404 2211 9 -9.992613634934365 2211 10 -8.436388078872078 2211 11 -10.447263104044271 2211 12 -6.0040158612305845 2211 13 -7.671990936024294 2211 14 -6.077005067232744 2211 15 -6.606698131735488 2211 16 -12.640847151230037 2211 17 -8.934072062586033 2211 18 -7.194872454122191 2211 19 -9.983750154394691 2211 20 -8.178572117440062 2211 21 -5.817225793759935 2211 22 -7.0500889110124625 2211 23 -8.723489799310144 2211 24 -22.35479792403758 2211 25 -22.159682830239465 2212 1 -1.2312348219469271 2212 2 -13.933061646791941 2212 3 -8.04740523092804 2212 4 -20.032818596975776 2212 5 -20.264366774663838 2212 6 -21.174387976754993 2212 7 -10.406723401965175 2212 8 -9.858178517620805 2212 9 -5.607319882605463 2212 10 -5.835306087677058 2212 11 -5.98132512532527 2212 12 -3.21542645204746 2212 13 -6.8027172778175835 2212 14 -4.337840850227263 2212 15 -4.265186380262239 2212 16 -4.2878903066696665 2212 17 -4.565205531168267 2212 18 -3.613027548727416 2212 19 -4.125115616242081 2212 20 -4.258326185257726 2212 21 -2.054161164107306 2212 22 -3.406766480396527 2212 23 -3.6263438271051704 2212 24 -2.5011896766148993 2212 25 -3.0545281910642075 2213 1 -18.682093928476135 2213 2 -13.933061646791941 2213 3 -8.67182443375949 2213 4 -5.56126171074823 2213 5 -20.264366774663838 2213 6 -10.925136479749005 2213 7 -21.10981618547033 2213 8 -10.893189938078859 2213 9 -8.28483341224721 2213 10 -7.181902070968206 2213 11 -6.778495218129354 2213 12 -5.00670626355846 2213 13 -19.410277415274628 2213 14 -12.443992978959491 2213 15 -6.827081661863986 2213 16 -6.292135939116115 2213 17 -4.926937736859976 2213 18 -4.80579819300587 2213 19 -5.687391065343878 2213 20 -5.71105834979463 2213 21 -3.7878628329346 2213 22 -4.736650526736838 2213 23 -4.541836038558554 2213 24 -3.7380314569717115 2213 25 -4.6619283392215 2214 1 -18.682093928476135 2214 2 -13.933061646791941 2214 3 -12.197535355013217 2214 4 -20.032818596975776 2214 5 -20.264366774663838 2214 6 -21.174387976754993 2214 7 -16.647054679194177 2214 8 -14.945647783893506 2214 9 -13.710924535440153 2214 10 -13.492396771942827 2214 11 -14.42059240450973 2214 12 -11.947629646917266 2214 13 -19.410277415274628 2214 14 -16.595518171252728 2214 15 -24.091303218750213 2214 16 -18.16840620193775 2214 17 -23.798195221889916 2214 18 -17.66504267475367 2214 19 -14.611308084861326 2214 20 -13.206457718421394 2214 21 -10.729092507476727 2214 22 -11.768698355764972 2214 23 -11.761707383734311 2214 24 -10.599149388472727 2214 25 -10.363866930657874 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 152378.0 3 5.0 4 789.0 5 2744.0 6 3727.0 7 3726.0 8 3159.0 9 3021.0 10 2786.0 11 2747.0 12 2538.0 13 2802.0 14 4332.0 15 8913.0 16 13524.0 17 14211.0 18 14896.0 19 17190.0 20 20301.0 21 23313.0 22 24414.0 23 26228.0 24 27329.0 25 28477.0 26 24131.0 27 19008.0 28 19818.0 29 23210.0 30 22575.0 31 25934.0 32 33549.0 33 42118.0 34 51014.0 35 59128.0 36 58543.0 37 62122.0 38 45302.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67161189082583 17.88936675301048 12.173829661237676 26.265191694926017 2 14.320524603634189 19.833254529452287 41.34565153100888 24.500569335904643 3 19.35893950018365 23.66233060003721 28.943841020430554 28.03488887934859 4 11.516280304716387 16.1256818439168 38.35324037739434 34.00479747397247 5 13.11012730436229 37.26170226866067 36.46918485965594 13.1589855673211 6 32.81836775087418 36.8009500560797 17.899716302698423 12.480965890347694 7 28.24343111186006 31.031816638622562 21.997070857009746 18.727681392507638 8 24.733483012473396 37.841818467547114 20.58494250591143 16.83975601406805 9 26.318962579199617 15.237371084448473 19.767032487450102 38.67663384890181 10 13.972760157369542 29.65567717126991 35.07970509740974 21.291857573950804 11 33.998933608161835 21.908030264534357 25.113885638421596 18.97915048888221 12 23.741574935686987 24.212806336172882 32.06781536026016 19.97780336787997 13 28.709926467631554 21.189010087579383 25.531387303536146 24.56967614125292 14 19.90540048384353 22.31285884395875 27.898180506288938 29.883560165908783 15 22.454585107872298 31.653262895561657 23.188581861348197 22.703570135217845 16 21.611867555559673 27.993759526335538 28.603097535777326 21.79127538232746 17 21.21486193436461 28.187711435593464 29.19392800346971 21.403498626572212 18 20.957990248754857 27.429540751489267 31.010967298877716 20.60150170087816 19 23.104338348299184 26.778344285994425 27.239176931461117 22.87814043424527 20 23.140299381968347 27.85694567293337 28.152721987166125 20.850032957932157 21 22.064706352978828 26.30528089305968 26.795908378552635 24.834104375408856 22 22.119706215515038 28.49611301932985 27.94994884349764 21.43423192165747 23 21.72104088048546 27.91075917687882 28.285876576814704 22.082323365821015 24 22.57325622238823 26.98248183126951 28.716117605734837 21.72814434060742 25 22.31346951814154 27.385474395328878 28.336503430115954 21.964552656413623 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 641.0 1 641.0 2 1593.5 3 2546.0 4 2546.0 5 2546.0 6 3757.0 7 4968.0 8 4968.0 9 4968.0 10 5722.0 11 6476.0 12 6476.0 13 6476.0 14 7876.0 15 9276.0 16 9276.0 17 9276.0 18 13293.0 19 17310.0 20 17310.0 21 17310.0 22 24853.0 23 32396.0 24 32396.0 25 32396.0 26 43495.0 27 54594.0 28 54594.0 29 54594.0 30 67273.0 31 79952.0 32 79952.0 33 79952.0 34 89261.5 35 98571.0 36 98571.0 37 98571.0 38 103774.0 39 108977.0 40 108977.0 41 108977.0 42 111014.0 43 113051.0 44 113051.0 45 113051.0 46 111123.0 47 109195.0 48 109195.0 49 109195.0 50 100732.0 51 92269.0 52 92269.0 53 92269.0 54 79600.5 55 66932.0 56 66932.0 57 66932.0 58 55639.0 59 44346.0 60 44346.0 61 44346.0 62 35270.0 63 26194.0 64 26194.0 65 26194.0 66 19748.0 67 13302.0 68 13302.0 69 13302.0 70 9495.5 71 5689.0 72 5689.0 73 5689.0 74 3960.5 75 2232.0 76 2232.0 77 2232.0 78 1494.0 79 756.0 80 756.0 81 756.0 82 496.0 83 236.0 84 236.0 85 236.0 86 154.5 87 73.0 88 73.0 89 73.0 90 43.5 91 14.0 92 14.0 93 14.0 94 9.0 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.289970134898574 2 36.55149089552608 3 5.781335322841971 4 13.67199174833315 5 13.53131790715077 6 14.848505958413577 7 9.319080181842288 8 7.7679600719998385 9 0.10685369246361243 10 1.4416821535232505 11 5.804593697542253 12 5.745605066056032 13 16.17254792685859 14 1.584153743474734 15 2.88684744528664 16 2.9894314844236303 17 2.8513419070968378 18 1.5051651569322315 19 1.4487607893015972 20 0.2816847602589657 21 4.502237073624554 22 1.6020188718677035 23 0.10640425527133647 24 9.153912013680868 25 0.10842672263657833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 890002.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.3680189890676 #Duplication Level Percentage of deduplicated Percentage of total 1 96.50553749442275 89.14025319835015 2 2.619970610216253 4.840029901505077 3 0.41707444962333934 1.1557302204799056 4 0.15523410021377193 0.5735466526518603 5 0.08982613256891846 0.41485309594201814 6 0.042874140257133785 0.23761196408465285 7 0.034158084924185816 0.2208580245835165 8 0.02154640531939138 0.1592159020550152 9 0.017980639051181992 0.14947524083836333 >10 0.08390947291553101 1.4468769256798175 >50 0.00588275726726115 0.38034033347003765 >100 0.005505237188887987 0.9289513359931558 >500 5.004760314402751E-4 0.3522572043664417 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 981 0.11022447140568224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.123592980689931E-4 0.0 7 0.0 0.0 0.0 1.123592980689931E-4 0.0 8 0.0 0.0 0.0 1.123592980689931E-4 0.0 9 0.0 0.0 0.0 1.123592980689931E-4 0.0 10 0.0 0.0 0.0 1.123592980689931E-4 1.123592980689931E-4 11 0.0 0.0 0.0 1.123592980689931E-4 1.123592980689931E-4 12 0.0 0.0 0.0 1.123592980689931E-4 2.247185961379862E-4 13 0.0 0.0 0.0 1.123592980689931E-4 2.247185961379862E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 690 0.0 15.3173 2 GTCCTAC 760 0.0 14.781961 1 TAGGACC 305 0.0 14.781652 4 ACGTGCA 40 0.004396313 14.646591 8 CTGTAGG 695 0.0 13.85524 1 GTAGGAC 690 0.0 13.667715 3 AACTGCG 45 0.0071938876 13.5980215 7 CTAGGAC 105 4.1676685E-8 13.5769005 3 TCCTACA 875 0.0 13.490592 2 GCCATAT 210 0.0 13.374156 1 AGGACGT 520 0.0 12.844855 5 GTTCTAA 95 7.724804E-5 12.670252 1 CTACACT 120 2.3266693E-8 12.523345 4 GTCCTAA 310 0.0 12.511307 1 GTCTAGG 75 0.002412261 12.482545 1 GTCTTAA 75 0.002412261 12.482545 1 TAGGACA 260 0.0 12.385726 4 CCTTATA 90 5.5989216E-4 12.200793 2 ATAGGAC 90 2.1974089E-5 12.184399 3 TACACTT 60 0.002908504 12.175852 5 >>END_MODULE