FastQCFastQC Report
Thu 26 May 2016
SRR1926457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890002
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA20720.2328084655989537No Hit
GTCCTACAGTGGACATTTCTAAATT20430.22955004595495293No Hit
GTCCTACAGTGTGCATTTCTCATTT14260.16022435904638416No Hit
CTTTAGGACGTGAAATATGGCGAGG14030.15764009519079733No Hit
GTCCTAAAGTGTGTATTTCTCATTT13890.15606706501783144No Hit
CCCATGTACTCTGCGTTGATACCAC11650.13089858225037695No Hit
CTGTAGGACCTGGAATATGGCGAGA11350.12752780330830718No Hit
GTACATGGGAAGCAGTGGTATCAAC10330.11606715490526988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTCG250.00609899218.9626610
TCGTCAA400.00512657214.3121362
GCTCCCG400.005350052414.22039611
TAGGACC4800.013.3137174
TGTAGGA12200.013.2954272
CTGTAGG12000.012.9509061
GTATAAA905.1646566E-712.7125461
TAGGACT1750.012.5359014
GTAGGAC11800.012.4488443
AGGACGT9450.012.4147815
TTAGGAC5550.012.3745613
GGACGTG8950.012.3623416
TAGGACG9950.012.2702294
GTCCTAC10250.012.09242251
AGGACCT7400.011.9871795
GGCGAGG4000.011.84034319
GACGTGG5300.011.8335577
TAGGACA3150.011.8091814
CTAGGAC1700.011.7831313
CGTGGAA5250.011.7652559
Produced by FastQC (version 0.11.5)