FastQCFastQC Report
Thu 26 May 2016
SRR1926456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910400
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10340.11357644991212654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC701.5655351E-615.7311253
ACAGCGC400.004363852614.6629838
AGAGCGC400.004363852614.6629838
TAGGACC2300.014.5126714
ATTAGGG550.001008821414.0150023
GTCCTAC7900.013.9279841
TGTAGGA6200.013.5487182
GTCTAAA600.00785192913.4184241
GTACTGG1408.8402885E-913.4184241
GTATAAT600.00785192913.4184241
CTGTAGG6200.013.2019981
GTCCTAA2750.012.6865091
TGTAGAA2400.012.62326052
GACAGCG654.329641E-412.5802017
TACTGGA1009.494605E-512.3937462
GTCCAGG1307.057879E-712.3862371
GTAGGAC6500.012.3670853
GTGTATA2500.012.3449491
GTCTTAC1102.1696836E-512.1985661
CTATACT801.1765742E-412.11339954
Produced by FastQC (version 0.11.5)