##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926456_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 910400 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.191132469244288 27.0 7.0 31.0 2.0 31.0 2 16.432024384885764 16.0 2.0 30.0 2.0 31.0 3 20.244892355008787 25.0 15.0 31.0 2.0 31.0 4 22.530690905096662 28.0 7.0 35.0 2.0 37.0 5 22.889283831282953 30.0 7.0 35.0 2.0 37.0 6 23.783606107205625 31.0 2.0 35.0 2.0 37.0 7 23.693000878734622 31.0 7.0 35.0 2.0 37.0 8 23.89113137082601 31.0 10.0 35.0 2.0 37.0 9 26.631801405975395 32.0 17.0 37.0 2.0 39.0 10 27.319343145869947 32.0 25.0 38.0 2.0 39.0 11 24.39145869947276 30.0 8.0 37.0 2.0 39.0 12 25.53361269771529 31.0 16.0 37.0 2.0 39.0 13 22.119340949033393 28.0 2.0 37.0 2.0 39.0 14 25.56551955184534 31.0 17.0 38.0 2.0 40.0 15 26.687228690685412 31.0 17.0 38.0 2.0 40.0 16 27.07489565026362 32.0 18.0 39.0 2.0 40.0 17 26.382735061511422 31.0 17.0 38.0 2.0 40.0 18 26.48790202108963 31.0 17.0 38.0 2.0 40.0 19 26.88779767135325 31.0 18.0 39.0 2.0 40.0 20 27.12214960456942 31.0 21.0 39.0 2.0 40.0 21 24.94996704745167 30.0 8.0 38.0 2.0 40.0 22 25.91337214411248 31.0 10.0 38.0 2.0 40.0 23 25.919395869947277 31.0 2.0 38.0 2.0 40.0 24 24.914610061511425 31.0 2.0 39.0 2.0 40.0 25 24.728516036906854 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.978289873853697 1101 2 10.949185346125287 1101 3 7.6255792790056525 1101 4 9.19656985669176 1101 5 8.849188232572217 1101 6 8.43463173724367 1101 7 8.155242124160917 1101 8 6.392699840998862 1101 9 6.524860703282425 1101 10 6.018703674368169 1101 11 8.879084076024768 1101 12 6.447884303728628 1101 13 11.152010451377109 1101 14 8.501349354629589 1101 15 7.634585421423292 1101 16 7.091056231214509 1101 17 2.969306130349242 1101 18 5.501391895032846 1101 19 6.354309693837962 1101 20 4.294715867093991 1101 21 1.5662556060700439 1101 22 5.417816541271765 1101 23 6.621347366160524 1101 24 7.949477337706924 1101 25 8.472178470554681 1102 1 5.811444703324881 1102 2 11.076082195630505 1102 3 7.481987117883289 1102 4 8.8063792483 1102 5 8.41402645747359 1102 6 7.4036977207043755 1102 7 7.2753510480609265 1102 8 7.579063776359664 1102 9 6.447354888554248 1102 10 5.6558789730318395 1102 11 8.286479364534792 1102 12 5.663009849570951 1102 13 10.250515066896654 1102 14 7.719416419233369 1102 15 5.502170694264336 1102 16 6.012029057585629 1102 17 6.936155854834858 1102 18 6.886267023850788 1102 19 5.506457390463481 1102 20 5.84930937585024 1102 21 4.851781107384788 1102 22 5.893035426361944 1102 23 6.4546379710103245 1102 24 7.843851683075634 1102 25 7.619641494955459 1103 1 5.094632614935218 1103 2 9.74167352158689 1103 3 6.626907386662747 1103 4 8.353220643902802 1103 5 7.559386891621507 1103 6 7.124894747638038 1103 7 7.3406013851495935 1103 8 6.965133378205014 1103 9 5.910826734685809 1103 10 5.245455411369683 1103 11 6.136678274707631 1103 12 4.718543118272407 1103 13 9.053426844384703 1103 14 7.228331160873019 1103 15 6.204435038640806 1103 16 6.032120656555975 1103 17 3.6085613864320436 1103 18 2.284755975142872 1103 19 4.507326577773348 1103 20 4.926299313335612 1103 21 7.332358114037412 1103 22 2.67623448139933 1103 23 4.797420930481678 1103 24 6.252910174924217 1103 25 2.3678724759113408 1104 1 5.655814162360993 1104 2 10.525579831738423 1104 3 7.144183277579202 1104 4 8.01573583365641 1104 5 7.8893009122044475 1104 6 7.589966272648979 1104 7 7.53722290381511 1104 8 7.491008059713604 1104 9 6.154604718742885 1104 10 5.3381957808642895 1104 11 8.286614035260662 1104 12 5.369768973302204 1104 13 9.943794492660679 1104 14 7.542583235019812 1104 15 6.6862516886056405 1104 16 6.2360251925357915 1104 17 6.741393367702223 1104 18 4.316692339253841 1104 19 5.173000047836748 1104 20 4.245370088780227 1104 21 3.2623504867968336 1104 22 5.449936333116774 1104 23 5.872595302274217 1104 24 7.043897891968449 1104 25 6.028794306669788 1105 1 3.4247954538561345 1105 2 9.529868638148319 1105 3 6.475296772970918 1105 4 5.9433189005525975 1105 5 2.368270770471341 1105 6 5.056605553676569 1105 7 5.882833947957476 1105 8 2.9085503007505693 1105 9 3.667934305875388 1105 10 3.26728450577982 1105 11 6.525744497049619 1105 12 4.0606684797395545 1105 13 4.920484951981617 1105 14 3.6301341845243655 1105 15 3.9675774296018176 1105 16 4.3023540661785376 1105 17 4.976259454825826 1105 18 2.7591864311196836 1105 19 2.633113982585769 1105 20 1.6597627052585686 1105 21 2.518631619154359 1105 22 4.102186061986526 1105 23 1.4503787301804856 1105 24 4.648931411079662 1105 25 5.4631070768942 1106 1 6.672954191953014 1106 2 11.510023960080744 1106 3 8.234268734571685 1106 4 9.640750777586781 1106 5 9.121279342692578 1106 6 8.209651653776952 1106 7 8.331292909087534 1106 8 7.942842809630221 1106 9 6.923741966636246 1106 10 6.084195164059892 1106 11 8.601076882522143 1106 12 5.514837557330861 1106 13 5.045555870412034 1106 14 6.335404636916504 1106 15 6.5319403596272245 1106 16 6.606891194820648 1106 17 7.0411343665930275 1106 18 6.995494895790525 1106 19 6.693525814820042 1106 20 6.381445162223315 1106 21 6.793666278083517 1106 22 6.984131085254212 1106 23 7.3327103067097745 1106 24 8.12631341468991 1106 25 7.231814946111957 1107 1 4.291210947648729 1107 2 -14.105795248958803 1107 3 -5.606660251959699 1107 4 2.3119347182168966 1107 5 4.951492108483798 1107 6 5.143074988832975 1107 7 5.222029489592394 1107 8 5.6719044205751175 1107 9 3.8961285474731433 1107 10 3.2320390095084974 1107 11 5.972653525981336 1107 12 3.451194932921254 1107 13 4.70622789108743 1107 14 4.143363070495898 1107 15 3.9971437951617297 1107 16 3.7913201155304534 1107 17 4.452971546289046 1107 18 -0.6378066791224306 1107 19 2.186965337685912 1107 20 2.573572277156373 1107 21 -0.6815541460574117 1107 22 2.5895582985094663 1107 23 -0.679593053784231 1107 24 3.0742862469824033 1107 25 3.8107127453276455 1108 1 5.050943703114438 1108 2 10.521910378746824 1108 3 -0.02056601905241706 1108 4 5.474049753742904 1108 5 7.326935582247124 1108 6 7.257459374708159 1108 7 7.287557969468814 1108 8 6.929257166504627 1108 9 5.629502442372505 1108 10 4.874902886971004 1108 11 7.01590893415807 1108 12 2.599183845066978 1108 13 3.6747103228110376 1108 14 5.124491166923132 1108 15 4.9145698366876935 1108 16 4.843177731349311 1108 17 0.2897312544150985 1108 18 3.4873592326911833 1108 19 4.698516340391684 1108 20 4.996426987858889 1108 21 5.015404005874515 1108 22 2.004809447336932 1108 23 4.880880816139367 1108 24 5.5535338491742685 1108 25 5.9630160380878685 1109 1 3.539869136749207 1109 2 -14.105795248958803 1109 3 -5.90505591023587 1109 4 2.16932492297736 1109 5 1.404090776218748 1109 6 2.3187477714999503 1109 7 3.5889731323363883 1109 8 3.943481434809218 1109 9 3.074856800710542 1109 10 2.3720882005876405 1109 11 0.983741089776764 1109 12 1.2874205121226439 1109 13 2.6634956373803256 1109 14 3.144151102317526 1109 15 3.1937151977145852 1109 16 3.061677337983891 1109 17 3.549442537691391 1109 18 3.568530247018259 1109 19 2.0690756972888344 1109 20 0.8590634650194815 1109 21 0.2806355040053319 1109 22 2.5545394105090367 1109 23 3.027465602463746 1109 24 4.174785267234782 1109 25 4.459849664758281 1110 1 3.113562948104505 1110 2 9.31328921267885 1110 3 6.498046067948728 1110 4 7.413943699715876 1110 5 8.278303363402138 1110 6 3.8137561680870213 1110 7 6.402704221393183 1110 8 7.230524288440442 1110 9 5.405446862693825 1110 10 5.032773385267035 1110 11 3.0186326542070177 1110 12 5.695250898580841 1110 13 10.266639163979999 1110 14 7.712472587731298 1110 15 6.926043671869731 1110 16 6.419830055722002 1110 17 6.801632726802893 1110 18 6.88533815346646 1110 19 5.609791488748897 1110 20 6.046019745658334 1110 21 3.961969092257231 1110 22 5.823741275387302 1110 23 6.618535258960986 1110 24 7.379584191941937 1110 25 7.783803153707808 1111 1 5.07068433695175 1111 2 9.708319432830738 1111 3 7.392439464976562 1111 4 7.958261104548907 1111 5 8.417664638949713 1111 6 7.272300506211458 1111 7 7.426239253977808 1111 8 7.569019240779259 1111 9 5.864161832851504 1111 10 5.442485273078908 1111 11 7.278962290919232 1111 12 6.509420426381066 1111 13 8.073542204764713 1111 14 6.154095103359367 1111 15 5.670139498819605 1111 16 5.4921975386191875 1111 17 6.679133552540051 1111 18 6.327671229632937 1111 19 5.441309419128594 1111 20 4.686041001534477 1111 21 7.4564655997851474 1111 22 6.831573285712977 1111 23 6.692446961758979 1111 24 6.615630452723746 1111 25 7.265313344705998 1112 1 -2.329441686992798 1112 2 7.372465620606416 1112 3 5.411343056658982 1112 4 -0.6655574209709414 1112 5 3.510922357916318 1112 6 2.391393133810702 1112 7 5.0639204133980975 1112 8 5.444885655398036 1112 9 4.021061643815585 1112 10 2.6831910071683893 1112 11 -0.8747869214311166 1112 12 3.3997795946230305 1112 13 7.577007863239821 1112 14 5.006016968513745 1112 15 3.1647834811762507 1112 16 3.4903071794509444 1112 17 4.307993035841882 1112 18 4.190935249796773 1112 19 3.9069678942388606 1112 20 3.5524556203120454 1112 21 -2.4880838646130847 1112 22 1.7767528698339596 1112 23 3.410783445690928 1112 24 5.107753956189406 1112 25 4.872118637711669 1113 1 -18.860002493546045 1113 2 -14.105795248958803 1113 3 -7.6868784199911175 1113 4 -5.958169997492931 1113 5 -1.151880965443599 1113 6 -0.5603075634939714 1113 7 -21.282015314390183 1113 8 -9.872434708318751 1113 9 -5.262345759882507 1113 10 -4.455944852486006 1113 11 -5.752814521509567 1113 12 -3.5295939996049057 1113 13 -2.3279112435759046 1113 14 -2.6638528762931912 1113 15 -2.928575223865124 1113 16 -3.2689987707988095 1113 17 -3.019804841781358 1113 18 -3.605763507072993 1113 19 -3.223714345982806 1113 20 -3.9600925254250505 1113 21 -1.2860699852872735 1113 22 -2.485522989069299 1113 23 -2.68739212819791 1113 24 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3.328792854519534 2204 4 6.140147175288266 2204 5 1.0659169398725474 2204 6 4.321559519922108 2204 7 5.531544007643717 2204 8 5.44412244650308 2204 9 3.9545256838606377 2204 10 3.351446117751525 2204 11 5.289738816946791 2204 12 5.149320149440573 2204 13 3.223787557118296 2204 14 4.64640468520469 2204 15 4.613643966754559 2204 16 4.692602721210136 2204 17 -0.613387821827466 2204 18 2.8823556809563975 2204 19 3.793941093201422 2204 20 4.187640536215298 2204 21 5.815927130852845 2204 22 4.929541293376285 2204 23 3.448119066186333 2204 24 5.236289753479809 2204 25 5.5583637896099525 2205 1 6.04442788620079 2205 2 10.64104019407917 2205 3 7.523637968641626 2205 4 8.113277734923866 2205 5 8.265765107152596 2205 6 7.72827069117951 2205 7 7.945832786875641 2205 8 7.608628247503194 2205 9 6.281421529915466 2205 10 5.621735590132822 2205 11 7.749964420564368 2205 12 7.018343799037062 2205 13 10.195280138769643 2205 14 7.736080060248014 2205 15 6.698099266871733 2205 16 6.138721909895295 2205 17 6.957649261664031 2205 18 6.770508602911075 2205 19 5.131977665082665 2205 20 5.803609377500592 2205 21 -0.42012983942424853 2205 22 4.143264000619695 2205 23 5.485740573621186 2205 24 7.393307065710594 2205 25 7.629927336554317 2206 1 4.197713943091344 2206 2 8.987680284290885 2206 3 6.28372325682675 2206 4 1.9520338297732245 2206 5 5.8041146140058295 2206 6 6.247562179831579 2206 7 6.313342276575941 2206 8 6.330259999484518 2206 9 4.9737789461758055 2206 10 3.9988118094997525 2206 11 3.5473541573939364 2206 12 4.684389730774747 2206 13 8.21824362526171 2206 14 0.1330560620268315 2206 15 2.5188368429084527 2206 16 3.5778258280694644 2206 17 4.832173188414661 2206 18 -0.5209379609438827 2206 19 1.4041351226371006 2206 20 2.9992417330109724 2206 21 3.993492446680289 2206 22 -1.5670912874066047 2206 23 -2.6748076848798377 2206 24 1.385562040854726 2206 25 3.1730359876668786 2207 1 1.4275374425561935 2207 2 -14.105795248958803 2207 3 -6.460462407465464 2207 4 -4.505693430199276 2207 5 0.9472832856619178 2207 6 1.8313624532210042 2207 7 2.781243471552308 2207 8 2.807888165811118 2207 9 0.14083431755650366 2207 10 -0.03923622205628163 2207 11 -1.8335859494764755 2207 12 -2.8158030849359257 2207 13 0.26958150974064665 2207 14 -6.221003289936 2207 15 -2.223435897119046 2207 16 -0.8792139496913869 2207 17 0.24136139819494673 2207 18 0.16254362535617872 2207 19 -0.1025857676022568 2207 20 -0.4022587019267547 2207 21 -4.223357080901177 2207 22 -4.799155217239324 2207 23 -1.8164634948060403 2207 24 -0.045433986580423635 2207 25 0.4883149290645328 2208 1 4.612438451335844 2208 2 6.541691122937017 2208 3 6.362172727015913 2208 4 8.122120093601769 2208 5 3.7284789207983344 2208 6 6.1169223714040015 2208 7 6.664683655930833 2208 8 6.586388561543785 2208 9 5.153916910544005 2208 10 4.571862095741238 2208 11 6.7847392789164225 2208 12 6.0971740438445785 2208 13 8.851932800370971 2208 14 3.015882864839014 2208 15 4.596479228383362 2208 16 5.211281693379032 2208 17 6.0194027827367975 2208 18 0.8505824361843537 2208 19 3.3205710760572167 2208 20 4.1479676107156 2208 21 6.861097494987575 2208 22 6.0128344182415425 2208 23 2.041945098688462 2208 24 3.5539657382450756 2208 25 5.026949854552619 2209 1 3.191050473907687 2209 2 4.766572332406863 2209 3 5.258778485472142 2209 4 -0.0847213441839969 2209 5 4.904033022139714 2209 6 5.473108040625764 2209 7 5.845617104244152 2209 8 4.471165709233663 2209 9 4.198960486076153 2209 10 3.357090981794709 2209 11 -0.3935085544826862 2209 12 3.2864264046771225 2209 13 2.092223827023421 2209 14 3.6979664350834227 2209 15 4.092924499316219 2209 16 3.671080767019692 2209 17 1.1937744868383113 2209 18 3.4281964366335025 2209 19 4.002082194417927 2209 20 3.9328420481685633 2209 21 6.384224203211442 2209 22 5.1320953561890725 2209 23 0.88508787112589 2209 24 -2.913444123775079 2209 25 0.4060556313690533 2210 1 -2.3553667319566394 2210 2 -14.105795248958803 2210 3 -7.9935009365474095 2210 4 -2.374064037228031 2210 5 -0.9399604356422735 2210 6 -1.4530227952820525 2210 7 -1.52969743359548 2210 8 -21.514156562623388 2210 9 -13.508703375776546 2210 10 -7.509587236591965 2210 11 -10.721026442039369 2210 12 -5.119660224770467 2210 13 -5.86897084622491 2210 14 -4.761203869670673 2210 15 -6.492813634461154 2210 16 -5.720654399937882 2210 17 -5.123116100059502 2210 18 -5.235564831576305 2210 19 -10.624376597638436 2210 20 -7.467377293636972 2210 21 -5.887394488598531 2210 22 -10.302079280460026 2210 23 -8.136398750714466 2210 24 -11.002237405745522 2210 25 -8.622771967662294 2211 1 0.02838167257984736 2211 2 4.756631746498691 2211 3 0.626749954118754 2211 4 1.2723992982684003 2211 5 0.5970418549491114 2211 6 -0.25194969834233305 2211 7 -21.282015314390183 2211 8 -13.684176030501389 2211 9 -9.17779285495709 2211 10 -7.319921972357424 2211 11 -9.605302585078851 2211 12 -4.91789479336143 2211 13 -6.904309557219319 2211 14 -4.78966436019244 2211 15 -5.428394297228515 2211 16 -12.357307300135998 2211 17 -7.898272423252063 2211 18 -6.112171146388654 2211 19 -8.897638563077582 2211 20 -7.023436187191084 2211 21 -4.580241225257879 2211 22 -6.144538593454513 2211 23 -7.932603588890416 2211 24 -22.631376478593204 2211 25 -22.461844815344413 2212 1 -0.9622305407282212 2212 2 -14.105795248958803 2212 3 -7.956192646684286 2212 4 -20.17406403722803 2212 5 -20.439298183986644 2212 6 -21.360969815149602 2212 7 -10.291189626316788 2212 8 -9.650460625532956 2212 9 -5.330418807194878 2212 10 -5.41464395762171 2212 11 -5.7990020177122545 2212 12 -3.110216845985086 2212 13 -6.642325415587742 2212 14 -4.045707354520474 2212 15 -3.933702249461195 2212 16 -4.00705176829041 2212 17 -4.250060976071758 2212 18 -3.2010999708401044 2212 19 -3.7702047593910173 2212 20 -3.966155209323567 2212 21 -1.8800133770920162 2212 22 -3.1754711720000444 2212 23 -3.329212061712358 2212 24 -2.1634865703363246 2212 25 -2.694479153483339 2213 1 -18.860002493546045 2213 2 -14.105795248958803 2213 3 -8.702470177611627 2213 4 -5.424238253255908 2213 5 -20.439298183986644 2213 6 -10.94912312525413 2213 7 -21.282015314390183 2213 8 -10.958059001647777 2213 9 -8.288741218728298 2213 10 -7.038474792260331 2213 11 -6.515176936997488 2213 12 -5.147220969630517 2213 13 -19.690162899205042 2213 14 -12.663276002028706 2213 15 -7.061685499149526 2213 16 -6.249435810741581 2213 17 -4.9705097821325595 2213 18 -4.9880257771886 2213 19 -5.94924449343187 2213 20 -5.790890688657392 2213 21 -3.8485777730898434 2213 22 -4.8838431312111155 2213 23 -4.579191283653987 2213 24 -3.737996687652437 2213 25 -4.495294292696329 2214 1 -18.860002493546045 2214 2 -14.105795248958803 2214 3 -12.576921632550887 2214 4 -20.17406403722803 2214 5 -20.439298183986644 2214 6 -21.360969815149602 2214 7 -16.88742071979559 2214 8 -15.458558107024933 2214 9 -14.263258593245672 2214 10 -14.09949748742937 2214 11 -14.985842724736447 2214 12 -12.81050376462063 2214 13 -19.690162899205042 2214 14 -17.20281833181494 2214 15 -24.35506529009029 2214 16 -18.691643174900165 2214 17 -24.042983649728377 2214 18 -18.222416962799297 2214 19 -15.353538683091912 2214 20 -14.1079638593891 2214 21 -11.610834106077883 2214 22 -12.667419739177967 2214 23 -12.627802263972285 2214 24 -11.480797328014052 2214 25 -11.220145973645572 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 156347.0 3 6.0 4 838.0 5 2701.0 6 3668.0 7 3686.0 8 3273.0 9 2996.0 10 2819.0 11 2635.0 12 2581.0 13 2702.0 14 4229.0 15 9159.0 16 13947.0 17 14741.0 18 15180.0 19 17472.0 20 20666.0 21 24010.0 22 24791.0 23 27080.0 24 28038.0 25 29014.0 26 24466.0 27 18555.0 28 19775.0 29 23289.0 30 22343.0 31 26116.0 32 33600.0 33 42602.0 34 52260.0 35 60875.0 36 60573.0 37 64985.0 38 48382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32555347158822 18.19876464453926 12.215146919696803 26.260534964175708 2 14.268721474347219 20.231751005657532 41.097376294953285 24.402151225041965 3 18.89109174621682 24.298360085004074 29.33762014302087 27.472928025758243 4 11.452038030085603 16.543624891349012 38.66464576898016 33.33969130958522 5 12.874076890977745 37.50256817958498 36.50464696942048 13.118707960016792 6 31.9850961082985 37.18627398814074 18.280627666160093 12.548002237400672 7 27.732847601700062 31.250030358227082 22.66241651487553 18.35470552519733 8 24.703706265518303 37.5663962715527 20.987065398091413 16.742832064837586 9 26.373080183902083 15.139372573254587 19.925731006370157 38.56181623647318 10 14.074738575655738 29.601090774387334 35.283248758150684 21.04092189180625 11 33.52796489854582 22.015762936650287 25.496929791249173 18.959342373554726 12 23.752402544152414 24.44564547280548 32.189470802727584 19.61248118031452 13 28.650415340014295 21.382426725263596 25.708214736986495 24.258943197735615 14 20.024558220188243 22.422057259311565 27.995924095211706 29.557460425288482 15 22.400082501986056 31.84905309705429 23.235528437686632 22.515335963273017 16 21.437169321164806 27.974466040105362 28.623582643073142 21.964781995656686 17 21.03792758887119 28.516838453712662 29.184802680920196 21.260431276495954 18 20.948669112376887 27.325117950069107 31.245883190915 20.480329746639008 19 23.09275957762808 26.77964286072952 27.32596350944066 22.80163405220174 20 23.100598260187553 27.884917255912377 28.252840111081856 20.76164437281821 21 22.03194057310435 26.417717404084907 26.930348155474555 24.619993867336188 22 22.267558947055495 28.594211353329175 27.87526849995418 21.26296119966115 23 21.54649049209315 28.148968386883233 28.377360482339476 21.927180638684145 24 22.65323835892761 27.16880585363015 28.59245215316805 21.58550363427419 25 22.186653939795153 27.538977876797137 28.54022930362166 21.734138879786048 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 844.0 1 844.0 2 1907.5 3 2971.0 4 2971.0 5 2971.0 6 4301.5 7 5632.0 8 5632.0 9 5632.0 10 6457.0 11 7282.0 12 7282.0 13 7282.0 14 8717.5 15 10153.0 16 10153.0 17 10153.0 18 14626.0 19 19099.0 20 19099.0 21 19099.0 22 27275.5 23 35452.0 24 35452.0 25 35452.0 26 46914.5 27 58377.0 28 58377.0 29 58377.0 30 71297.5 31 84218.0 32 84218.0 33 84218.0 34 93043.5 35 101869.0 36 101869.0 37 101869.0 38 106797.5 39 111726.0 40 111726.0 41 111726.0 42 112873.0 43 114020.0 44 114020.0 45 114020.0 46 111659.5 47 109299.0 48 109299.0 49 109299.0 50 100204.5 51 91110.0 52 91110.0 53 91110.0 54 78660.5 55 66211.0 56 66211.0 57 66211.0 58 55063.5 59 43916.0 60 43916.0 61 43916.0 62 34797.5 63 25679.0 64 25679.0 65 25679.0 66 19470.5 67 13262.0 68 13262.0 69 13262.0 70 9572.0 71 5882.0 72 5882.0 73 5882.0 74 4072.0 75 2262.0 76 2262.0 77 2262.0 78 1527.5 79 793.0 80 793.0 81 793.0 82 518.5 83 244.0 84 244.0 85 244.0 86 159.0 87 74.0 88 74.0 89 74.0 90 47.5 91 21.0 92 21.0 93 21.0 94 12.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.73451230228471 2 37.1143453427065 3 5.979898945518453 4 14.194749560632689 5 13.92475834797891 6 15.36280755711775 7 9.545254833040422 8 7.894771528998243 9 0.11017135325131809 10 1.554920913884007 11 5.996814586994727 12 6.104679261862917 13 16.72078207381371 14 1.6898066783831285 15 3.0752416520210897 16 3.0891915641476273 17 3.0445957820738134 18 1.6126977152899826 19 1.5622803163444638 20 0.28569859402460457 21 4.713752196836555 22 1.7150702987697715 23 0.10973198594024605 24 9.38884007029877 25 0.11401581722319859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 910400.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.84941829040044 #Duplication Level Percentage of deduplicated Percentage of total 1 96.31728224007415 88.46686345063127 2 2.751946237936215 5.055293222417944 3 0.4406835960727113 1.2142959584820097 4 0.16900004800795698 0.6209022440232238 5 0.08361874519543107 0.38401665521867795 6 0.05681578150700706 0.3131097888679853 7 0.0310981542687844 0.1999443165645086 8 0.026150946916247496 0.19215594096003788 9 0.017170278636428952 0.141937209417608 >10 0.09241582464373368 1.531502846496071 >50 0.0071577926560456775 0.4468983246883998 >100 0.006043654756847122 0.9959057054194302 >500 4.933594627805938E-4 0.32003563138026286 >1k 1.2333986569514844E-4 0.11713870543263426 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1034 0.11357644991212654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0984182776801405E-4 0.0 10 0.0 0.0 0.0 1.0984182776801405E-4 0.0 11 0.0 0.0 0.0 1.0984182776801405E-4 0.0 12 0.0 0.0 0.0 1.0984182776801405E-4 0.0 13 0.0 1.0984182776801405E-4 0.0 1.0984182776801405E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTATAC 70 1.5655351E-6 15.731125 3 ACAGCGC 40 0.0043638526 14.662983 8 AGAGCGC 40 0.0043638526 14.662983 8 TAGGACC 230 0.0 14.512671 4 ATTAGGG 55 0.0010088214 14.015002 3 GTCCTAC 790 0.0 13.927984 1 TGTAGGA 620 0.0 13.548718 2 GTCTAAA 60 0.007851929 13.418424 1 GTACTGG 140 8.8402885E-9 13.418424 1 GTATAAT 60 0.007851929 13.418424 1 CTGTAGG 620 0.0 13.201998 1 GTCCTAA 275 0.0 12.686509 1 TGTAGAA 240 0.0 12.6232605 2 GACAGCG 65 4.329641E-4 12.580201 7 TACTGGA 100 9.494605E-5 12.393746 2 GTCCAGG 130 7.057879E-7 12.386237 1 GTAGGAC 650 0.0 12.367085 3 GTGTATA 250 0.0 12.344949 1 GTCTTAC 110 2.1696836E-5 12.198566 1 CTATACT 80 1.1765742E-4 12.1133995 4 >>END_MODULE