FastQCFastQC Report
Thu 26 May 2016
SRR1926456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910400
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA21880.24033391915641478No Hit
GTCCTACAGTGGACATTTCTAAATT20570.22594463971880493No Hit
CTTTAGGACGTGAAATATGGCGAGG17340.19046572934973638No Hit
GTCCTAAAGTGTGTATTTCTCATTT17100.18782952548330403No Hit
GTCCTACAGTGTGCATTTCTCATTT13550.14883567662565905No Hit
CCCATGTACTCTGCGTTGATACCAC12650.1389499121265378No Hit
GTACATGGGAAGCAGTGGTATCAAC11640.12785588752196836No Hit
CTGTAGGACCTGGAATATGGCGAGA11620.12763620386643235No Hit
CTGAAGGACCTGGAATATGGCGAGA10340.11357644991212654No Hit
GTATCAACGCAGAGTACTTTTTTTT9890.1086335676625659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC508.446896E-515.2542491
TAGGACC3700.014.6898054
GGCGAGG4550.012.28524619
TAGGACT1850.011.854934
CAGGACT1700.011.7791314
GTCTAAT657.781635E-411.7340381
TGTAGGA11750.011.5275612
GTACCAT752.002487E-411.4419526
GTAGGAC11450.011.1606683
AGAACGT600.005711690511.1247365
TGTAGTG600.005711690511.1247365
CTGCTAG600.00571874711.1228911
TGGCGAG11600.011.107718
CTGTAGG11250.011.1017051
GGACTCA951.3041472E-511.0404816
GTCCTAA5200.011.0006611
AGGACCT8100.010.9481535
TAAACTG1053.3091928E-610.89775
GTCTTAG1407.874405E-910.8958931
GTCCTAC10250.010.8826061
Produced by FastQC (version 0.11.5)