FastQCFastQC Report
Thu 26 May 2016
SRR1926455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890857
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16680.18723543733730555No Hit
GTCCTAAAGTGTGTATTTCTCATTT12800.14368187037874766No Hit
CTGTAGGACGTGGAATATGGCAAGA12020.13492625640254272No Hit
GTCCTACAGTGTGCATTTCTCATTT10340.11606801091533209No Hit
CTTTAGGACGTGAAATATGGCGAGG9320.10461836186952563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG552.3344466E-416.990991
TAATACT659.593423E-716.4872134
CTGTAGG10050.014.8776841
TGTAGGA10600.014.8398572
TAGGACC4200.014.5440774
GTCTAAT658.314637E-414.3769911
GTGTAGT658.314637E-414.3769911
TTAGCCT450.005179920214.2889174
GTACTAA802.6194047E-413.3500641
TCCAACG901.0506483E-813.2572518
CCATGTA2900.013.2068052
TGTAGAA3800.012.9585572
TCTAGAT600.002046192312.7598633
TTTGGCG651.5217287E-512.62961116
GTAGGAC11800.012.5126973
GTCATAT750.002445152312.460061
AGGACCT7200.012.4432185
GTCCTAC9050.012.391221
GGACCTG7450.012.2977436
GTAGAAC3750.012.2494673
Produced by FastQC (version 0.11.5)