FastQCFastQC Report
Thu 26 May 2016
SRR1926455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890857
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA34650.3889513131737193No Hit
GTCCTACAGTGGACATTTCTAAATT32340.3630212256288046No Hit
CTTTAGGACGTGAAATATGGCGAGG26260.2947723371988995No Hit
GTCCTAAAGTGTGTATTTCTCATTT24880.2792816355486908No Hit
GTCCTACAGTGTGCATTTCTCATTT21870.24549394571743835No Hit
CTGTAGGACCTGGAATATGGCGAGA17210.19318476478267554No Hit
CTGAAGGACCTGGAATATGGCGAGA15600.17511227952409872No Hit
CCCATGTACTCTGCGTTGATACCAC14870.16691792285406074No Hit
GTACATGGGAAGCAGTGGTATCAAC13760.15445801065715373No Hit
GTCCTTCAGTGTGCATTTCTCATTT9090.10203657826115753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC1600.014.3188573
CCAACGT801.3345198E-714.20714819
TCCAACG1750.013.53061718
AGCGGAC500.001501579313.2965738
TAGGACC6550.013.2584994
GTCCTAG1259.458745E-1112.9750911
TCAATAC603.9259726E-412.7278723
TAGGACT1501.8189894E-1212.7242744
CTACTAG603.9447547E-412.7206761
CACGTTT905.5969576E-712.62857518
TGTAGGA17250.012.61790852
CTACACT1900.012.55684954
GCTTTAT701.04743136E-412.2663671
CTGTAGG17350.012.1524621
TTAGACG550.002964170912.1458974
CCAACGA1103.9681254E-812.0545519
ACGTTTT951.0765561E-611.96391419
GTAGGAC17250.011.8977943
AGGACCT11500.011.7837855
ACTGTTC2850.011.6636618
Produced by FastQC (version 0.11.5)