FastQCFastQC Report
Thu 26 May 2016
SRR1926454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672677
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG908.521965E-917.760481
GTCCTAT500.002453805215.9844321
GTCCTAA1601.8189894E-1214.9854061
TCCTAAA1601.4551915E-1114.518842
TAAAGTA450.00627811913.8795675
CCTAGGA1006.73306E-613.6647912
TGTAGGA2950.013.4331852
GTTCTAA600.00823708713.320361
GTTTAGA600.00823708713.320361
GGTTTAA600.00823708713.320361
GTACTGG1008.765048E-613.320361
GTGTAGG1409.769792E-913.320361
GTATATG904.826684E-513.320361
GTATAAT700.00147880513.320361
GTAGGAC2650.012.8019033
CTATTAA854.4498796E-412.536811
CTAAAAC704.54358E-412.5069283
TAGGACA1305.4224074E-912.3697064
TAGACAG706.9219834E-411.8967725
GTATTAA907.197742E-411.8403211
Produced by FastQC (version 0.11.5)