##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926454_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 672677 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.448714613402867 27.0 7.0 31.0 2.0 31.0 2 16.47044123702758 18.0 2.0 30.0 2.0 31.0 3 20.084866882619743 24.0 15.0 31.0 2.0 31.0 4 22.3827750911656 28.0 7.0 35.0 2.0 37.0 5 22.983008189666066 30.0 7.0 35.0 2.0 37.0 6 23.717390367145004 31.0 2.0 35.0 2.0 37.0 7 23.470817346215195 30.0 7.0 35.0 2.0 37.0 8 23.594814450323113 31.0 10.0 35.0 2.0 37.0 9 26.34723500283197 32.0 17.0 37.0 2.0 39.0 10 27.09758621151013 32.0 24.0 37.0 2.0 39.0 11 24.150691936843387 30.0 7.0 37.0 2.0 39.0 12 25.316654204023624 31.0 12.0 37.0 2.0 39.0 13 21.947288223025314 27.0 2.0 37.0 2.0 39.0 14 25.308474944141096 31.0 17.0 38.0 2.0 40.0 15 26.40510676000517 31.0 17.0 38.0 2.0 40.0 16 26.78949778273971 31.0 17.0 39.0 2.0 40.0 17 26.096176917004744 31.0 13.0 38.0 2.0 40.0 18 26.177527996348918 31.0 17.0 38.0 2.0 40.0 19 26.584442459010788 31.0 18.0 39.0 2.0 40.0 20 26.842588642097173 31.0 18.0 39.0 2.0 40.0 21 24.678813159956412 30.0 7.0 38.0 2.0 40.0 22 25.615815614329016 31.0 8.0 38.0 2.0 40.0 23 25.613579771569416 31.0 2.0 38.0 2.0 40.0 24 24.655839280962482 31.0 2.0 39.0 2.0 40.0 25 24.447891038343812 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.185282786675504 1101 2 10.854434471669023 1101 3 7.2740257879916825 1101 4 8.737593688084882 1101 5 8.751343716273436 1101 6 8.27544609208184 1101 7 8.064767982618381 1101 8 6.551477128963171 1101 9 6.523266094553843 1101 10 5.9604579581563435 1101 11 8.946192082359847 1101 12 6.626375324016482 1101 13 10.968906740536482 1101 14 8.236083754248561 1101 15 7.682100396294121 1101 16 7.0786210760929436 1101 17 2.905347428893972 1101 18 5.437181080776167 1101 19 6.273098171867407 1101 20 4.137726690669972 1101 21 1.1721592303935822 1101 22 4.875155020610144 1101 23 6.201360913523981 1101 24 7.411151693392501 1101 25 8.231887663638137 1102 1 6.016388918331884 1102 2 10.787924115708286 1102 3 7.233090604228721 1102 4 8.48444714453925 1102 5 8.234710480220176 1102 6 7.271405806686801 1102 7 7.383547704993703 1102 8 7.1840664052656855 1102 9 6.319702270694027 1102 10 5.7113885941511775 1102 11 8.140119004846003 1102 12 5.74807555682607 1102 13 10.212385512096969 1102 14 7.7755002545151015 1102 15 5.666655302311934 1102 16 6.158914783608203 1102 17 6.68226961387068 1102 18 6.703291022317774 1102 19 5.503769404372804 1102 20 5.823057277271825 1102 21 4.82583840492849 1102 22 5.948563075882092 1102 23 6.451375972358846 1102 24 7.822178826401164 1102 25 7.689425514019579 1103 1 4.83319924482117 1103 2 9.142222268565018 1103 3 6.16930502415271 1103 4 7.740009498678052 1103 5 7.071776803021205 1103 6 6.693769552893539 1103 7 6.668419602409529 1103 8 5.866773774732245 1103 9 5.050866692667906 1103 10 4.374064294768388 1103 11 5.2282209083032 1103 12 4.2947685445816575 1103 13 8.154105237365446 1103 14 6.3363831145819525 1103 15 5.313194896495819 1103 16 5.122408970045985 1103 17 3.0658107853086847 1103 18 1.867534307771372 1103 19 3.6179721948517276 1103 20 4.24758617026221 1103 21 6.550465615230845 1103 22 2.232939905794691 1103 23 4.102941275243467 1103 24 5.6207698952286975 1103 25 1.706256262043368 1104 1 5.761670760062977 1104 2 10.225537731439989 1104 3 6.899822274224004 1104 4 7.566110751566484 1104 5 7.542822522049445 1104 6 7.317311605656162 1104 7 7.186583136290789 1104 8 7.034850890839056 1104 9 6.058289516742086 1104 10 4.852696117427225 1104 11 7.942951207345569 1104 12 5.177220807884616 1104 13 9.341222856583975 1104 14 6.872754200105074 1104 15 6.1379959324556985 1104 16 5.906096665163176 1104 17 6.0222452413582666 1104 18 3.9007756176496358 1104 19 4.562962033144771 1104 20 3.922831409383992 1104 21 3.115684511415896 1104 22 5.103042365597446 1104 23 5.27414327613285 1104 24 6.587554168322409 1104 25 5.6764016861902356 1105 1 3.2868302126061906 1105 2 9.396776678250532 1105 3 6.04434591043837 1105 4 5.231636822176416 1105 5 1.922509796413376 1105 6 4.586126492633795 1105 7 5.563393264431916 1105 8 2.8228063137648896 1105 9 3.6173157291934963 1105 10 3.13724258022334 1105 11 6.25533525102248 1105 12 4.091920202914228 1105 13 4.9261316565464455 1105 14 3.8113379404538072 1105 15 4.226022679286622 1105 16 4.363551919125388 1105 17 4.871419738627363 1105 18 2.6901861068817787 1105 19 2.477262659373057 1105 20 1.5999481643442266 1105 21 2.740922471566872 1105 22 3.920792561866463 1105 23 1.6238913700692947 1105 24 4.670450431990687 1105 25 5.286209346216374 1106 1 6.670952068198979 1106 2 11.234932965930788 1106 3 8.14934910384542 1106 4 9.494365260653534 1106 5 9.114706116162385 1106 6 7.920339755516057 1106 7 7.689533906189773 1106 8 7.472139738777244 1106 9 6.587069450522829 1106 10 5.820783384593309 1106 11 8.279958156080113 1106 12 5.8588518172187065 1106 13 5.03619751657952 1106 14 6.142093392106474 1106 15 6.2101307830266315 1106 16 6.220126474369913 1106 17 6.595732659910766 1106 18 6.362162595466675 1106 19 6.359889887049153 1106 20 6.060550169421074 1106 21 6.275942557069371 1106 22 6.702239469436293 1106 23 7.0713171153807295 1106 24 8.136908316232695 1106 25 7.282481459939945 1107 1 3.8758012835752815 1107 2 -13.889551782626508 1107 3 -5.498368207847983 1107 4 1.7699249927536727 1107 5 4.546141722516801 1107 6 4.776788043662851 1107 7 4.5091106123284455 1107 8 5.2237784185528895 1107 9 3.5698524158038936 1107 10 3.041041578335406 1107 11 5.699947543873879 1107 12 3.132735287506243 1107 13 4.722990536685739 1107 14 4.117213740428461 1107 15 3.9322921036628387 1107 16 3.4942254836456392 1107 17 4.073492674408165 1107 18 -0.5686967529877904 1107 19 1.9022388364338738 1107 20 2.2269421497232784 1107 21 -1.1745943340758913 1107 22 1.7762132738984988 1107 23 -0.716131239349771 1107 24 2.527817787798366 1107 25 3.3505251965073697 1108 1 5.061056404717164 1108 2 10.40856451548979 1108 3 0.25413124490126293 1108 4 5.749376323578858 1108 5 7.001326829658613 1108 6 6.9606922567094465 1108 7 6.764711834624091 1108 8 6.781056989986332 1108 9 5.218070083172268 1108 10 4.152735287031611 1108 11 6.651016166049914 1108 12 2.2484592167189312 1108 13 3.087336791956222 1108 14 4.555350537432869 1108 15 4.478044653578589 1108 16 4.32894825425517 1108 17 -0.24526639955490737 1108 18 3.259493787109495 1108 19 4.136105509101547 1108 20 4.4373796707353215 1108 21 4.3123033576511105 1108 22 1.3785471193630698 1108 23 4.094099246983962 1108 24 4.899953756936831 1108 25 5.429235198614538 1109 1 3.89818169011831 1109 2 -13.889551782626508 1109 3 -5.600319057824903 1109 4 2.447480021682555 1109 5 2.2006655462387066 1109 6 2.8940164658957244 1109 7 4.02563419899473 1109 8 4.151249705006634 1109 9 3.6775816806148995 1109 10 2.8577320609939036 1109 11 1.0112722504439269 1109 12 1.6804397866305365 1109 13 3.243296706536828 1109 14 3.8335023328260434 1109 15 3.7945613149228343 1109 16 3.667799524140925 1109 17 4.0325703955232655 1109 18 3.9978175943988177 1109 19 2.3965542867917016 1109 20 1.4662836651565563 1109 21 0.9197847237531676 1109 22 2.8181463520657495 1109 23 3.2918086515899176 1109 24 4.425852213869771 1109 25 4.8621368728955225 1110 1 3.195686618453138 1110 2 9.346045809806853 1110 3 6.4278368820634775 1110 4 7.682195721574523 1110 5 8.47625012315456 1110 6 3.873314798738697 1110 7 6.578010633305521 1110 8 7.50208404377349 1110 9 5.695561644928663 1110 10 5.447646707480434 1110 11 3.0395140988298763 1110 12 5.919951157342428 1110 13 10.299304485437244 1110 14 7.774364595655868 1110 15 7.046315026664093 1110 16 6.581092761773711 1110 17 7.143130830630803 1110 18 7.267142254775155 1110 19 6.534012444411747 1110 20 6.371229333733741 1110 21 4.152788335711325 1110 22 6.427241401075406 1110 23 6.963530235073591 1110 24 8.047062641792923 1110 25 8.330061452837182 1111 1 4.854276890309819 1111 2 9.504413012008865 1111 3 7.3489740111606245 1111 4 8.076577105096487 1111 5 8.336491759123625 1111 6 7.502338070777725 1111 7 7.465325501708836 1111 8 7.496892539015512 1111 9 6.177908150302873 1111 10 5.234209049142422 1111 11 7.070691957058479 1111 12 6.543029028472315 1111 13 8.464024307788751 1111 14 6.238097217664883 1111 15 5.705682258416527 1111 16 5.451745516197445 1111 17 6.697702172919115 1111 18 6.347406968903645 1111 19 5.32518897647504 1111 20 4.683960016225978 1111 21 7.575614523728412 1111 22 7.097940138839913 1111 23 6.865497788709412 1111 24 6.9506583516481335 1111 25 7.370942660858464 1112 1 -2.835560011390779 1112 2 6.696888895339594 1112 3 4.981553890967977 1112 4 -1.0388085485043206 1112 5 3.2445145474226003 1112 6 2.0905809281235435 1112 7 4.867802521613083 1112 8 4.856652153717089 1112 9 3.4901511781347203 1112 10 2.4235018162727187 1112 11 -1.1296859536352564 1112 12 2.779884555771389 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3.8908923869095773 2204 7 4.726813096725351 2204 8 4.751190760120103 2204 9 2.8914042565064406 2204 10 2.348831383127706 2204 11 4.126362141395887 2204 12 4.3130657813254984 2204 13 2.4983807030001337 2204 14 3.3171745992569335 2204 15 3.5748442503781845 2204 16 3.754477979784898 2204 17 -1.0991419534304612 2204 18 2.3256690532847593 2204 19 2.649457322453358 2204 20 3.185263918619569 2204 21 4.67144991679767 2204 22 4.022626163442112 2204 23 2.754850207734922 2204 24 4.463812720795797 2204 25 4.626710996090338 2205 1 5.8522650139544155 2205 2 10.343809632706265 2205 3 7.008404090159786 2205 4 7.482193693295976 2205 5 7.851157130205003 2205 6 7.303761143165023 2205 7 7.462638527496022 2205 8 7.194455760588134 2205 9 5.948090292552209 2205 10 5.300127835435113 2205 11 7.52473410547973 2205 12 6.802567433843155 2205 13 10.003608275203277 2205 14 7.2954988959468565 2205 15 5.98338333836125 2205 16 5.652660570385347 2205 17 6.444969827328329 2205 18 5.997727463843592 2205 19 4.7784091438230405 2205 20 5.087210937247296 2205 21 -1.4368112313960815 2205 22 3.4355942867724956 2205 23 4.604674155199984 2205 24 6.499625190643648 2205 25 6.950463327256315 2206 1 3.8611198503398185 2206 2 8.508474533162964 2206 3 5.804552329861821 2206 4 1.769047160683904 2206 5 5.422034618787453 2206 6 5.837363934367968 2206 7 5.942348128214331 2206 8 5.730010204564547 2206 9 4.4108107454853 2206 10 3.564712231080925 2206 11 2.998213243510147 2206 12 4.073503534361933 2206 13 7.580638631475168 2206 14 -0.00821901297878469 2206 15 2.135847118124474 2206 16 2.9088203661996523 2206 17 3.8015735773837527 2206 18 -1.555197174785416 2206 19 0.6126939396373459 2206 20 2.005896228561088 2206 21 3.1676150090153925 2206 22 -2.409364671015826 2206 23 -3.1209679434121753 2206 24 0.6962252826491486 2206 25 2.2317576672554296 2207 1 1.0528808373476402 2207 2 -13.889551782626508 2207 3 -6.309368137237918 2207 4 -4.33079292283724 2207 5 0.9135720265385707 2207 6 1.6678262283270442 2207 7 2.8814493644598542 2207 8 3.1341742473857046 2207 9 0.5012236282905498 2207 10 0.22392234310531833 2207 11 -1.5847941805916683 2207 12 -2.786365853370622 2207 13 0.2906367257347462 2207 14 -5.804685935953728 2207 15 -1.874325807104217 2207 16 -0.6309619651008909 2207 17 0.7074997396092186 2207 18 0.6692458794812346 2207 19 0.09764135224947879 2207 20 -0.03541898090464102 2207 21 -3.9879873154355323 2207 22 -4.677375989456767 2207 23 -1.499628898381463 2207 24 0.35133667179581707 2207 25 0.8811276681392499 2208 1 4.7925090203803045 2208 2 6.618140525065801 2208 3 6.262978443222146 2208 4 7.9508538408458485 2208 5 3.0560427159534456 2208 6 5.933897335177683 2208 7 6.45506320918599 2208 8 6.4587930993013885 2208 9 4.860140198926594 2208 10 4.431197554967564 2208 11 6.713901502619461 2208 12 5.9126609230259035 2208 13 8.706612821758569 2208 14 3.2187040639442905 2208 15 4.511821308407875 2208 16 4.956682208304915 2208 17 5.600498172525455 2208 18 0.6198660843238954 2208 19 3.041135235183905 2208 20 4.173343090909267 2208 21 6.782291122375717 2208 22 5.899909114409276 2208 23 1.9441889391789182 2208 24 3.5430749790054215 2208 25 4.823382161182554 2209 1 3.1291425302445433 2209 2 4.6752872077999506 2209 3 5.108868726270597 2209 4 0.4482833958540766 2209 5 4.995027152340658 2209 6 5.53899664702428 2209 7 6.149640081171135 2209 8 4.362911096644616 2209 9 4.427265986885143 2209 10 3.5670791347958257 2209 11 -0.3566777372053487 2209 12 3.295157232085355 2209 13 2.6397410832639103 2209 14 3.8934460504631083 2209 15 4.236255511006156 2209 16 3.7963047633871376 2209 17 1.438098902609351 2209 18 3.1818918557216627 2209 19 3.968180403839625 2209 20 4.029331223522384 2209 21 6.38980478502199 2209 22 5.032811162661055 2209 23 0.6844277668163983 2209 24 -2.439617240597613 2209 25 0.40564027684729353 2210 1 -1.8842365844114433 2210 2 -13.889551782626508 2210 3 -7.441153329835368 2210 4 -1.7405073334280878 2210 5 -0.156816618895796 2210 6 -0.8723437681073989 2210 7 -0.7706813418649787 2210 8 -21.004364795435453 2210 9 -12.866504653457262 2210 10 -6.8601488299620925 2210 11 -9.726125857667453 2210 12 -4.321953537255464 2210 13 -4.911981081334254 2210 14 -3.65211471112827 2210 15 -5.065724769642891 2210 16 -4.725173114934691 2210 17 -4.222136918891181 2210 18 -4.36930811566501 2210 19 -9.856045546063207 2210 20 -6.919911119432182 2210 21 -4.780768792955286 2210 22 -9.55929632806942 2210 23 -7.463660505296339 2210 24 -10.536842940133837 2210 25 -7.89400086434755 2211 1 -0.2915267892078255 2211 2 4.376087383284641 2211 3 0.24666445230300837 2211 4 1.232518494844328 2211 5 0.46941735486609204 2211 6 -0.58983073805652 2211 7 -20.836434766522512 2211 8 -13.568281386550593 2211 9 -9.732252282381285 2211 10 -7.943774640847309 2211 11 -10.174801739550405 2211 12 -5.697151109671086 2211 13 -7.480406753121864 2211 14 -5.634942780693631 2211 15 -5.970230952448606 2211 16 -12.382984728756687 2211 17 -8.373829480067219 2211 18 -6.623620762369516 2211 19 -9.643455556950908 2211 20 -7.498376657586736 2211 21 -5.2586924300264535 2211 22 -6.702665387960046 2211 23 -8.559674282582858 2211 24 -22.15328129629822 2211 25 -21.944229174849834 2212 1 -1.0896313026715738 2212 2 -13.889551782626508 2212 3 -7.848201640936779 2212 4 -19.834571260368726 2212 5 -20.314807486475704 2212 6 -21.04585974984256 2212 7 -10.017315741217919 2212 8 -9.621984289343606 2212 9 -5.261332468395231 2212 10 -5.5856994926402805 2212 11 -5.632299014172485 2212 12 -3.0281857506476832 2212 13 -6.073736183549741 2212 14 -3.852013715292209 2212 15 -3.7936103191073123 2212 16 -4.047589483107309 2212 17 -4.100666316810493 2212 18 -3.2331563137880295 2212 19 -3.7259646210098225 2212 20 -4.065022450223502 2212 21 -1.9114865053140093 2212 22 -3.182561318034029 2212 23 -3.3496387740736466 2212 24 -2.3519692063263378 2212 25 -2.8458224269585486 2213 1 -18.91437357071281 2213 2 -13.889551782626508 2213 3 -8.45427251073042 2213 4 -5.511162636138746 2213 5 -20.314807486475704 2213 6 -10.623888497275825 2213 7 -20.836434766522512 2213 8 -10.527978758474468 2213 9 -8.11723927883114 2213 10 -7.107203296315596 2213 11 -6.326532784804993 2213 12 -5.005604255003679 2213 13 -19.283670579051147 2213 14 -12.22806662996787 2213 15 -6.669535257861693 2213 16 -6.078290233065452 2213 17 -4.570013678100953 2213 18 -4.6475000090013445 2213 19 -5.389913322872111 2213 20 -5.463834872162998 2213 21 -3.51612269767433 2213 22 -4.505206897861175 2213 23 -4.175892697545969 2213 24 -3.3432196946555095 2213 25 -4.136220961297472 2214 1 -18.91437357071281 2214 2 -13.889551782626508 2214 3 -12.00065329912095 2214 4 -19.834571260368726 2214 5 -20.314807486475704 2214 6 -21.04585974984256 2214 7 -16.41827333154493 2214 8 -14.671405154179848 2214 9 -13.318342399428555 2214 10 -13.216290689617683 2214 11 -14.022290650754663 2214 12 -11.769807623296787 2214 13 -19.283670579051147 2214 14 -16.454938389896416 2214 15 -23.89951472398079 2214 16 -17.92263098499223 2214 17 -23.6367487910373 2214 18 -17.568875042380412 2214 19 -14.680290483303414 2214 20 -13.087282170070017 2214 21 -10.667203258626806 2214 22 -11.57574492237292 2214 23 -11.56142551952735 2214 24 -10.404402372531406 2214 25 -10.106785228661494 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 117825.0 3 6.0 4 597.0 5 2466.0 6 3223.0 7 3095.0 8 2660.0 9 2425.0 10 2236.0 11 2054.0 12 1994.0 13 2072.0 14 3197.0 15 6923.0 16 10003.0 17 10664.0 18 11049.0 19 12565.0 20 15044.0 21 17359.0 22 18147.0 23 19869.0 24 20868.0 25 21742.0 26 18041.0 27 14264.0 28 14898.0 29 17436.0 30 16891.0 31 19575.0 32 25188.0 33 31632.0 34 38012.0 35 44133.0 36 44279.0 37 45489.0 38 34756.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.901873828964035 19.564207484001912 13.423479174630712 25.11043951240334 2 13.569865707086864 21.0709807282314 41.257416933843636 24.1017366308381 3 18.119886495379216 25.316293970027587 29.166870405042406 27.39694912955079 4 11.296703675906036 17.253034498822434 40.062458483225065 31.387803342046467 5 12.363430512312483 37.44589969894243 37.12854557462539 13.062124214119686 6 30.786717346402515 37.80779296967073 18.6860865268609 12.719403157065859 7 26.81533587358585 32.6033110651696 22.72368365010042 17.85766941114413 8 24.33305852725411 36.93780150842774 20.884832636656984 17.844307327661163 9 25.773091547671314 16.40015179813824 20.682496335265537 37.14426031892491 10 14.029887687250797 29.962844129463008 34.84562656388184 21.16164161940436 11 32.29820327165459 23.43713028252331 24.830975028788668 19.433691417033426 12 22.888281656217455 25.519681766530805 31.71334965380889 19.87868692344285 13 27.126324743912384 22.135185907205898 25.7535533264807 24.984936022401023 14 20.44505165670207 22.69146833193285 28.41118613943985 28.45229387192523 15 22.299099827561385 30.802344920105483 24.33130266192537 22.567252590407758 16 21.531794344299616 29.176049655620673 27.38064761402535 21.91150838605436 17 21.066520941154142 28.762573034963406 28.589753598363956 21.581152425518496 18 20.869391890769563 28.210614693015902 30.081557397471887 20.838436018742648 19 22.14247981284431 27.82627726209343 28.112293411817973 21.918949513244286 20 22.504118370541537 27.91651447951996 28.704856322910068 20.874510827028438 21 21.79432706898564 26.809851756740983 27.405470604256642 23.99035057001673 22 22.067221505077285 28.066399223669613 28.398788344161048 21.467590927092058 23 21.661599182058872 28.020138972602844 28.340112303197802 21.97814954214049 24 21.607449908299508 27.970367898120017 28.597465107185688 21.824717086394788 25 22.064063295321613 27.674587511794947 28.33183012897791 21.929519063905534 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 724.0 1 724.0 2 1554.5 3 2385.0 4 2385.0 5 2385.0 6 3391.5 7 4398.0 8 4398.0 9 4398.0 10 5214.0 11 6030.0 12 6030.0 13 6030.0 14 7664.0 15 9298.0 16 9298.0 17 9298.0 18 12875.5 19 16453.0 20 16453.0 21 16453.0 22 22596.5 23 28740.0 24 28740.0 25 28740.0 26 36939.0 27 45138.0 28 45138.0 29 45138.0 30 53548.5 31 61959.0 32 61959.0 33 61959.0 34 68385.0 35 74811.0 36 74811.0 37 74811.0 38 78189.5 39 81568.0 40 81568.0 41 81568.0 42 82258.5 43 82949.0 44 82949.0 45 82949.0 46 81262.0 47 79575.0 48 79575.0 49 79575.0 50 73226.0 51 66877.0 52 66877.0 53 66877.0 54 57524.5 55 48172.0 56 48172.0 57 48172.0 58 39632.0 59 31092.0 60 31092.0 61 31092.0 62 24424.0 63 17756.0 64 17756.0 65 17756.0 66 13382.0 67 9008.0 68 9008.0 69 9008.0 70 6355.5 71 3703.0 72 3703.0 73 3703.0 74 2535.0 75 1367.0 76 1367.0 77 1367.0 78 923.0 79 479.0 80 479.0 81 479.0 82 318.5 83 158.0 84 158.0 85 158.0 86 92.5 87 27.0 88 27.0 89 27.0 90 17.5 91 8.0 92 8.0 93 8.0 94 5.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.279181538836617 2 36.33749927528368 3 5.752240674201734 4 13.839034187284534 5 13.339537400565204 6 14.855569612161556 7 9.252434675185862 8 7.675749282345018 9 0.1088189428209973 10 1.4959631442728085 11 5.7601196413732 12 5.93657877406244 13 16.117244977901727 14 1.6354059972319552 15 2.9272592938364177 16 3.0243341157791925 17 2.88310734572462 18 1.55260251205259 19 1.5054773687817482 20 0.28275085962505037 21 4.482389021774194 22 1.650569292543078 23 0.11090017943232784 24 9.134844806645685 25 0.11580595144474987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 672677.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.09272703256303 #Duplication Level Percentage of deduplicated Percentage of total 1 97.49162024523511 92.70744031942438 2 1.9384369658081668 3.686625145188515 3 0.2843096717208553 0.8110734601700672 4 0.09921388915954456 0.3773807711875014 5 0.04872571792251364 0.23167306969356272 6 0.03257143867770811 0.18583841563422981 7 0.02116579361563194 0.14088991243031973 8 0.01678418501898358 0.12768431395793914 9 0.009792984200031444 0.08381174160310134 >10 0.051343080739869484 0.9131974388521411 >50 0.0030180865074580995 0.18533917786082327 >100 0.0030179413941005023 0.5490462339974492 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.486597579521821E-4 0.0 0.0 0.0 6 0.0 1.486597579521821E-4 0.0 0.0 0.0 7 0.0 1.486597579521821E-4 0.0 0.0 0.0 8 0.0 1.486597579521821E-4 0.0 0.0 0.0 9 0.0 1.486597579521821E-4 0.0 0.0 0.0 10 0.0 1.486597579521821E-4 0.0 0.0 0.0 11 0.0 1.486597579521821E-4 0.0 0.0 0.0 12 0.0 1.486597579521821E-4 0.0 0.0 0.0 13 0.0 1.486597579521821E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 90 8.521965E-9 17.76048 1 GTCCTAT 50 0.0024538052 15.984432 1 GTCCTAA 160 1.8189894E-12 14.985406 1 TCCTAAA 160 1.4551915E-11 14.51884 2 TAAAGTA 45 0.006278119 13.879567 5 CCTAGGA 100 6.73306E-6 13.664791 2 TGTAGGA 295 0.0 13.433185 2 GTTCTAA 60 0.008237087 13.32036 1 GTTTAGA 60 0.008237087 13.32036 1 GGTTTAA 60 0.008237087 13.32036 1 GTACTGG 100 8.765048E-6 13.32036 1 GTGTAGG 140 9.769792E-9 13.32036 1 GTATATG 90 4.826684E-5 13.32036 1 GTATAAT 70 0.001478805 13.32036 1 GTAGGAC 265 0.0 12.801903 3 CTATTAA 85 4.4498796E-4 12.53681 1 CTAAAAC 70 4.54358E-4 12.506928 3 TAGGACA 130 5.4224074E-9 12.369706 4 TAGACAG 70 6.9219834E-4 11.896772 5 GTATTAA 90 7.197742E-4 11.840321 1 >>END_MODULE