FastQCFastQC Report
Thu 26 May 2016
SRR1926454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672677
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15320.22774674918274299No Hit
GTACATGGGAAGCAGTGGTATCAAC9220.1370642968319119No Hit
CCCATGTACTCTGCGTTGATACCAC8970.13334780288310735No Hit
GGTATCAACGCAGAGTACTTTTTTT8210.12204966127874152No Hit
TATCAACGCAGAGTACTTTTTTTTT7220.10733234524147547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG453.4359895E-516.9443781
TAGGACC1800.013.2358174
GTTCTAG801.9224262E-613.1054171
GTATTAA905.179372E-712.7082841
GTGTAAC603.9750763E-412.7082841
GGCGAGG1207.6579454E-1012.63554619
GTAGGAC3300.011.5555763
CTCCTAG1001.8495411E-611.4374551
GTCCTAG855.1375588E-511.213191
GATATAC1451.1459633E-911.1745251
AGGACCT2750.011.0892075
AGGACGT2950.010.9834845
GTCCTAC4000.010.9608951
ACTGATT700.001486762410.8595288
GTATAGC1002.3076984E-510.4843341
ACTGTTC2001.8189894E-1210.4522958
GGACGTG3100.010.4520256
TGCGTTG2850.010.316380512
CCTACAC750.00256842610.1689083
CTATACT750.002576407310.1651084
Produced by FastQC (version 0.11.5)