FastQCFastQC Report
Thu 26 May 2016
SRR1926453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714548
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA10370.14512670947228176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAC250.003594167520.7757856
CTTATAC502.6792284E-517.441013
GTATAAT654.6650137E-516.3475191
GTATTAG708.787938E-515.1798391
TAGGACC1400.014.5218284
TGTAGGA2850.014.3239332
GTCCTAC3350.014.273581
TATACTG952.2577296E-814.224515
GTAGGAC2850.013.3867393
GTATTAT700.001511303913.282361
GCATAGA802.732589E-413.2823581
TCCTACA3700.013.239962
AGACTGT1306.348273E-1012.7850986
ATAGGAG600.002097118212.7174033
TAGGACG1800.012.4836784
TTAGGAC1056.5810127E-712.4578633
GTGTATA1604.9039954E-912.4522111
GCCTTAC750.00253881112.3968681
GTACTAA750.00253881112.3968681
AAAGTGT1203.6705387E-812.1192086
Produced by FastQC (version 0.11.5)