FastQCFastQC Report
Thu 26 May 2016
SRR1926453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714548
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA27420.3837390910057827No Hit
GCTTACTCTGCGTTGATACCACTGC15550.21762008990298762No Hit
CTGCTTACTCTGCGTTGATACCACT13270.18571180662460746No Hit
GCGTTGATACCACTGCTTACTCTGC10700.1497450136309947No Hit
CTGTAGGACGTGGAATATGGCAAGA7470.10454161231995612No Hit
GTATCAACGCAGAGTACTTTTTTTT7330.10258233176777488No Hit
GTCCTACAGTGGACATTTCTAAATT7150.1000632567721133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAG250.00609601518.96364211
TAGGACC2650.014.39694
GCTACAC1009.480573E-913.3540673
AGATAGT500.001453808613.3531266
AATCCCG802.0610114E-613.02473119
GGCGAGG1600.013.02473119
GTCCTAC3900.011.9801981
GTAGGAC4400.011.9232733
TGTAGGA5150.011.8563242
TTAGGAC2750.011.7932013
GTCCTAA1700.011.778851
AGGACCT4050.011.7752445
TAAGGGA657.750401E-411.7390124
GGACCTG3750.011.6998826
TAAACTG907.121318E-611.657495
TAGATAG751.9955123E-411.4455365
ACAGTCT752.0698868E-411.4005488
TGGCGAG4450.011.28189118
GGACAGT1950.011.24988656
TGGACCT600.00569868411.12760455
Produced by FastQC (version 0.11.5)