FastQCFastQC Report
Thu 26 May 2016
SRR1926452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710446
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTACA300.00213383222.7269952
CTTATAC452.3579097E-416.9686343
CCCTAGC758.694322E-615.9724881
GTCTAGC751.5528679E-414.1977671
GTCCAGG1151.7514867E-713.889121
TACACCC601.9201961E-413.8484855
ACCAGTA600.00705511113.6361972
CTGGAGT952.436791E-713.519784
GCTCTAC600.00827901313.3104071
GACCTAC600.00827901313.3104071
TAGGACC1553.6379788E-1213.1200014
GTCCTAC3900.012.9691141
TCTAGAG956.4345586E-512.9185032
TCCTACA4750.012.9185032
TGTAGGA3700.012.8991062
CTTGGAC851.2882749E-512.833423
CCAGTAC1005.1515217E-611.9992483
GTCTATG1001.302982E-411.9793651
GTATAGA907.244724E-411.8314711
CCTAGCA1051.6384176E-411.68816952
Produced by FastQC (version 0.11.5)