FastQCFastQC Report
Thu 26 May 2016
SRR1926452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710446
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA10640.14976507714872067No Hit
GTCCTACAGTGGACATTTCTAAATT10190.14343102783322026No Hit
GTCCTACAGTGTGCATTTCTCATTT7750.10908640487806251No Hit
GTATCAACGCAGAGTACTTTTTTTT7640.10753808171205131No Hit
CTTTAGGACGTGAAATATGGCGAGG7290.10261159891110654No Hit
GTCCTAAAGTGTGTATTTCTCATTT7260.10218932895673985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGC551.9864665E-413.79451310
TAGGACC2600.013.5648734
GTCCTAA2050.012.5535491
GTAGGAC5600.012.4292583
TCTATGG701.0527468E-412.2589953
CTGTAGG5800.012.1607491
TGTAGGA5900.012.1204742
GTGTAGC1750.011.9823291
CCTACAC1209.446012E-911.9184673
GTCCTAT802.7661701E-511.91424751
AGGACGT4750.011.8398385
GTGCAAA658.139693E-411.67063611
TAGGACG4750.011.4384884
GGACGTG4750.011.4376776
GACCTGG4250.011.1785887
GGACCTG4600.010.9818286
GACGTGA2600.010.9636147
ATAGCCC700.001444932510.8968843
CAGAACT1752.0008883E-1110.8937974
GTTCTTA700.001449208510.8930261
Produced by FastQC (version 0.11.5)