FastQCFastQC Report
Thu 26 May 2016
SRR1926451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700768
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAC405.705946E-419.8686581
GTCCAAG501.1947284E-418.5440831
CTGTAGG3050.016.502931
TGTAGGA2900.016.3784052
GTAGGAC3050.015.6845063
TGTACCG400.002955592715.5461325
CCTAGAC450.004705564614.49628453
GTTCTAT550.00480472214.4499351
GTATTGG550.00480472214.4499351
TAGGACA1353.6379788E-1214.2352884
TAGGACG2250.013.76077654
GTCCTAA1305.7403668E-813.2457731
GTCCTAG700.001542986613.2457721
TAGGACC1605.456968E-1212.6783014
GTGTAAG1152.8008726E-612.669871
TGTTAGA1101.9299729E-512.3369812
GGACGTG2100.012.3310566
AGGACGT2300.012.1665385
AGGACCT2400.012.0914375
TAAAGTG1800.012.0914355
Produced by FastQC (version 0.11.5)