FastQCFastQC Report
Thu 26 May 2016
SRR1926451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700768
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8510.1214381935248185No Hit
CTGTAGGACGTGGAATATGGCAAGA8060.11501666742773642No Hit
GTCCTACAGTGGACATTTCTAAATT7940.11330426046851454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1600.014.2958024
GTAAGAC759.307696E-713.9781173
AAGGCGT707.022869E-613.6130956
CTAGGAC1403.6379788E-1212.9342973
TAGAGTG905.184884E-712.7073795
AGGCGTG604.0703034E-412.6728227
GTCCTAC4150.012.6208671
TAGTATA550.00299111512.1306412
TTTAGAC550.002992723612.129773
CCCCTAT550.003010468812.1202041
AAAGGCG802.7686116E-511.9131685
AGGACGT3850.011.8822255
CTCTTAT657.852641E-411.7206371
GGACGTG3850.011.633016
TGTAGGA4600.011.6032232
GACGTGG2300.011.5708377
TAGGACG4300.011.5252974
GTAGGAC4550.011.3109643
GGCGAGG1354.8839865E-911.23257419
GTTTATA600.00576535911.1101881
Produced by FastQC (version 0.11.5)