##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926451_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 700768 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.3716993355861 31.0 28.0 31.0 22.0 34.0 2 28.84019104753642 31.0 28.0 33.0 23.0 34.0 3 29.20610102059455 31.0 29.0 33.0 25.0 34.0 4 33.00276981825654 35.0 33.0 37.0 27.0 37.0 5 32.71660949130097 35.0 33.0 37.0 26.0 37.0 6 32.76965129686287 35.0 33.0 37.0 26.0 37.0 7 32.627123384629435 35.0 33.0 37.0 25.0 37.0 8 32.58355118955203 35.0 33.0 37.0 25.0 37.0 9 33.853407975250015 37.0 34.0 39.0 24.0 39.0 10 33.755018779396316 37.0 33.0 39.0 23.0 39.0 11 33.973971414219825 37.0 34.0 39.0 25.0 39.0 12 33.7845535184255 37.0 34.0 39.0 25.0 39.0 13 33.9055921503265 37.0 34.0 39.0 25.0 39.0 14 34.58936909219599 38.0 34.0 40.0 23.0 41.0 15 34.69175961231107 39.0 34.0 40.0 24.0 41.0 16 34.668124115256404 39.0 34.0 40.0 24.0 41.0 17 34.58575305950043 38.0 34.0 40.0 24.0 41.0 18 34.54733520937029 38.0 34.0 40.0 23.0 41.0 19 34.594289408192154 39.0 34.0 40.0 23.0 41.0 20 34.573074112973195 39.0 34.0 40.0 22.0 41.0 21 34.488817982556284 39.0 34.0 40.0 21.0 41.0 22 34.41402290058907 39.0 34.0 40.0 19.0 41.0 23 34.08290190191333 39.0 34.0 40.0 18.0 41.0 24 34.14116940271245 39.0 34.0 40.0 18.0 41.0 25 33.95445425590209 39.0 34.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9396207396988387 1101 2 1.0493788511107702 1101 3 1.112742374690427 1101 4 0.7869310189216066 1101 5 0.9940080553915251 1101 6 0.5589645828878247 1101 7 1.0132206372019397 1101 8 0.9609453477348708 1101 9 1.4575391328107727 1101 10 1.4091110681065828 1101 11 1.1665221341507817 1101 12 1.2801875773374505 1101 13 1.114614885180515 1101 14 1.6696781449168299 1101 15 1.1639272512382277 1101 16 1.2666813028968633 1101 17 1.409140980445443 1101 18 1.466219421882073 1101 19 1.3514996740115635 1101 20 1.3408150159577445 1101 21 1.5210026359099658 1101 22 1.378348901794439 1101 23 1.6554417425696073 1101 24 1.5380185188797597 1101 25 1.6660402589730055 1102 1 0.883367677474439 1102 2 1.025539434158297 1102 3 0.8111183750170632 1102 4 0.6352488369846512 1102 5 0.8034305198697211 1102 6 0.39875920970474255 1102 7 0.7850032600992165 1102 8 0.8956025827503851 1102 9 1.098518840470419 1102 10 1.3330096880641236 1102 11 1.107451825478563 1102 12 1.064295653443935 1102 13 0.873765832428596 1102 14 1.223323212694062 1102 15 1.0114693057866475 1102 16 1.0962532039257695 1102 17 1.1468461494802327 1102 18 1.2579167107927347 1102 19 1.1795455665479082 1102 20 1.3114329728416294 1102 21 1.287064002886936 1102 22 1.3359268864424862 1102 23 1.4706824334074327 1102 24 1.365273336779481 1102 25 1.496678834834512 1103 1 0.34009844670520906 1103 2 0.16930192167078317 1103 3 0.20910580425449155 1103 4 0.03944880368461412 1103 5 0.2768633370525393 1103 6 -0.03584784990231782 1103 7 0.3886704262663798 1103 8 0.4563647372625397 1103 9 0.5336982443998224 1103 10 0.694887393691829 1103 11 0.28895781948455834 1103 12 0.3140354969337764 1103 13 0.30800867292143863 1103 14 0.6922813379021875 1103 15 0.5390875209048644 1103 16 0.6294387683613323 1103 17 0.42534889673297727 1103 18 0.33157222194824953 1103 19 0.49289887990121883 1103 20 0.4171085472671976 1103 21 0.6355592576321953 1103 22 0.4880664135820112 1103 23 0.6678290368040365 1103 24 0.4765266667828101 1103 25 0.5758704765261555 1104 1 0.4305261045789095 1104 2 0.5217251778710192 1104 3 0.49722141566147116 1104 4 0.2014497224301408 1104 5 0.36988697009415716 1104 6 -0.047518383605222425 1104 7 0.23353674100679456 1104 8 0.3727420250427045 1104 9 0.6738374317512381 1104 10 0.5945754354661972 1104 11 0.2777732606381278 1104 12 0.46382410760368487 1104 13 0.2550127579650905 1104 14 0.5750283870161397 1104 15 0.5001245354490749 1104 16 0.24185403095645341 1104 17 0.31814180526329494 1104 18 0.18676209602300986 1104 19 0.3874715334666803 1104 20 0.39034100026584184 1104 21 0.2959050070067164 1104 22 0.20022006728243014 1104 23 0.37467654580981957 1104 24 0.20848914874529356 1104 25 0.30414824392262574 1105 1 0.5296468080161603 1105 2 0.4417891267119458 1105 3 0.5611702565887278 1105 4 0.11511878717833923 1105 5 0.4346351715330101 1105 6 0.10196203206223942 1105 7 0.1583968529172708 1105 8 0.2168667252825145 1105 9 0.735388191143727 1105 10 0.5122413027723525 1105 11 0.3225374492872035 1105 12 0.5013940516484467 1105 13 0.16868182271329601 1105 14 0.7069228714286098 1105 15 0.536895118982244 1105 16 0.5967700981027377 1105 17 0.31894116577488774 1105 18 0.5464743262388438 1105 19 0.696824051452289 1105 20 0.8867438779636814 1105 21 0.6843942156398128 1105 22 0.6518587563311868 1105 23 1.050935538615576 1105 24 0.7488568545806231 1105 25 0.7973536795741083 1106 1 0.5597199606494314 1106 2 0.8372521984856007 1106 3 0.6316241723405902 1106 4 0.4592119588561374 1106 5 0.5682271027688941 1106 6 -0.07110875433652808 1106 7 -0.19034662242198408 1106 8 0.009097960214887735 1106 9 0.0895055347190663 1106 10 0.08959169337620665 1106 11 -0.2342467647758042 1106 12 -0.05132966579193976 1106 13 -0.18265400105796914 1106 14 0.15582844816244545 1106 15 0.12943845483149374 1106 16 0.09440099736738716 1106 17 0.07514257914618128 1106 18 -0.059772787623856516 1106 19 0.0334121514901895 1106 20 0.032282267968810174 1106 21 -0.004192126441900257 1106 22 0.03622313733634286 1106 23 0.2726150874068196 1106 24 0.07471314166251375 1106 25 0.21620330107212737 1107 1 0.893895915059641 1107 2 0.9415873893676441 1107 3 0.7926686022160254 1107 4 0.6004589960239244 1107 5 0.6149263816029276 1107 6 0.47030659691668575 1107 7 0.7266820981388165 1107 8 0.9495116220233513 1107 9 1.1616984087923896 1107 10 0.6670023040978776 1107 11 0.6900345908144914 1107 12 0.7702454262449763 1107 13 0.6016240765051961 1107 14 1.2021551666051309 1107 15 1.0280629442157334 1107 16 1.0480849955518678 1107 17 0.8643181553224935 1107 18 1.050841085995593 1107 19 1.042637906697209 1107 20 1.0177681724521435 1107 21 1.2614736912997913 1107 22 1.2074174739140844 1107 23 1.420931946552514 1107 24 1.3683871796876304 1107 25 1.338004703059113 1108 1 1.0742756618950828 1108 2 1.0816190349372619 1108 3 0.9374470832286832 1108 4 0.5759336795682231 1108 5 -0.72368121333378 1108 6 0.016983812106559526 1108 7 0.5107010854805836 1108 8 0.9732457992385406 1108 9 1.016128788539227 1108 10 1.5332997724523096 1108 11 1.0294333249917074 1108 12 0.9403403629538332 1108 13 1.031212684100133 1108 14 1.5518387109089247 1108 15 1.1127148429499059 1108 16 1.0836862613746518 1108 17 1.3026092945629983 1108 18 1.0397651366285032 1108 19 1.0711189193554347 1108 20 1.0715656408065755 1108 21 1.1633724254770073 1108 22 1.3688098789773733 1108 23 1.6669762503499825 1108 24 1.3066783189281352 1108 25 1.2525616650844356 1109 1 0.1902876813561747 1109 2 -0.7518571337397439 1109 3 0.22138489396209238 1109 4 0.2021899208517226 1109 5 -0.7795759658648933 1109 6 -0.08924110299069099 1109 7 0.40939134748141726 1109 8 0.3040171899402253 1109 9 0.5852892627170831 1109 10 -1.3955875224769514 1109 11 -0.34285790474075384 1109 12 0.4074984364333005 1109 13 0.319951891603516 1109 14 0.6141257886574891 1109 15 0.5053987013140286 1109 16 0.5079012436488739 1109 17 0.726840246006077 1109 18 0.6066273812548815 1109 19 0.4983547360719598 1109 20 0.7369663072532191 1109 21 0.6976203042390736 1109 22 0.5300352731496929 1109 23 0.6277047372180036 1109 24 0.5800432091134837 1109 25 0.6723139300448793 1110 1 0.6496778859575443 1110 2 -0.3476449165377389 1110 3 0.30945930589303927 1110 4 0.5407915030413335 1110 5 0.9327909374894858 1110 6 0.5762789251917511 1110 7 0.9132410659071155 1110 8 0.7724073395200932 1110 9 0.9573767854849038 1110 10 0.7092398636517743 1110 11 0.3753305588946745 1110 12 0.7523395993733928 1110 13 0.7265743362300938 1110 14 0.9512174952028261 1110 15 0.7846649574869744 1110 16 0.6161028052590183 1110 17 0.8498618837459659 1110 18 0.7818544163893222 1110 19 1.013532451936662 1110 20 0.8433814566008593 1110 21 0.7950598038149792 1110 22 0.8574832117371258 1110 23 1.2685694179182505 1110 24 1.1663858729971608 1110 25 1.2335124840513387 1111 1 -0.0380084298482295 1111 2 0.03137677722119747 1111 3 0.16877895008132526 1111 4 -0.09631968424832849 1111 5 0.3060215752517905 1111 6 0.16844273316829828 1111 7 0.26146535089436185 1111 8 0.19304551712890117 1111 9 0.5418588389833658 1111 10 0.4490083762717987 1111 11 0.09420776837576028 1111 12 0.11974609743518272 1111 13 0.04539354364309389 1111 14 0.029092310690792544 1111 15 0.02400303000222692 1111 16 0.2837671450385102 1111 17 0.19105341797809672 1111 18 0.351148234171319 1111 19 0.08075220304866093 1111 20 -0.07941807234351472 1111 21 0.1540996443374496 1111 22 0.08255446488448115 1111 23 0.3647001027720407 1111 24 0.1784790226291193 1111 25 0.4251755314546557 1112 1 0.30024034987716064 1112 2 0.19487213609742327 1112 3 0.5763534355880999 1112 4 0.8765216848935538 1112 5 0.7923423280115003 1112 6 0.6616038011177849 1112 7 1.0168426505362689 1112 8 1.0886821277184922 1112 9 0.9932940534321943 1112 10 1.3925707620763603 1112 11 0.9645453788367249 1112 12 0.9069487397844185 1112 13 0.7396547605566752 1112 14 0.858226202720175 1112 15 0.8997874428526984 1112 16 0.9375632398831328 1112 17 0.9577690016697744 1112 18 1.113153537127225 1112 19 1.1640282513644777 1112 20 1.0836374482493554 1112 21 1.019394329071602 1112 22 0.8171348826755249 1112 23 1.3382862924031969 1112 24 0.8535457245197335 1112 25 1.2623406215730952 1113 1 -0.028651553294789522 1113 2 0.1727921814110438 1113 3 -1.361592247693558 1113 4 -0.8988628846270714 1113 5 -1.2732178317786271 1113 6 -0.5436400576945246 1113 7 -0.20718996334400686 1113 8 -0.20897617182836825 1113 9 0.4155975950491708 1113 10 0.08449778330221847 1113 11 0.07077600130273964 1113 12 0.18879199044027217 1113 13 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0.868932572215698 2202 22 0.9754826597562172 2202 23 1.1673844177288473 2202 24 1.0483688373510986 2202 25 1.0434287940048534 2203 1 0.4238850575838704 2203 2 0.5771145065754197 2203 3 0.37382690950907005 2203 4 0.18983257263533204 2203 5 0.39763916164636015 2203 6 0.14870154421820558 2203 7 0.6131440175286755 2203 8 0.5260694049797578 2203 9 0.7940980433456488 2203 10 0.8879347229316252 2203 11 0.6796074418287361 2203 12 0.8322356545167793 2203 13 0.519284687701635 2203 14 1.056486902212356 2203 15 0.9921366161610763 2203 16 0.8675860812994429 2203 17 0.9520300085082312 2203 18 1.0040155735756997 2203 19 1.2057337437821047 2203 20 1.0724262943736491 2203 21 0.978504701309852 2203 22 1.0248443845650073 2203 23 1.3522663568062114 2203 24 1.1670200645983826 2203 25 1.143639997799859 2204 1 0.6984845226545744 2204 2 0.8403215665828334 2204 3 0.4991559439881783 2204 4 0.4129589960239244 2204 5 0.4226795461598911 2204 6 0.33581292603061286 2204 7 0.5478846297843845 2204 8 0.8498280777195504 2204 9 1.025622459425307 2204 10 0.8276035699206616 2204 11 0.720097881953734 2204 12 0.7014163123209229 2204 13 0.6759911651127908 2204 14 1.048674153946898 2204 15 0.9592338302916801 2204 16 0.9262495525138945 2204 17 0.9363118262085663 2204 18 0.8261575416917921 2204 19 0.8677961345453085 2204 20 0.941027666123027 2204 21 1.0518217925656117 2204 22 0.8434934232811742 2204 23 0.9929256174385941 2204 24 0.7117416100673779 2204 25 0.6940173612869671 2205 1 0.5864936142812027 2205 2 0.5738618006071228 2205 3 0.4227114211629157 2205 4 0.31441375494535606 2205 5 0.16671856171875277 2205 6 0.16850085089026834 2205 7 0.20408314098735758 2205 8 0.19660296746569372 2205 9 0.51429554071224 2205 10 0.49088348645331337 2205 11 0.24216838522434614 2205 12 0.46215311392317915 2205 13 0.2642300139342808 2205 14 0.6796524040924226 2205 15 0.6765171703668571 2205 16 0.5096416714597964 2205 17 0.6044144239571168 2205 18 0.6706200603140928 2205 19 0.7532110540871244 2205 20 0.6367091434753576 2205 21 0.8765946252264101 2205 22 0.760212914781512 2205 23 1.0280693420950087 2205 24 0.7438663029844932 2205 25 1.0118900785141918 2206 1 0.7260616416934624 2206 2 0.9128071439348133 2206 3 0.927832604079569 2206 4 0.8292456845641141 2206 5 1.1173103313268626 2206 6 0.8718720241938342 2206 7 0.8855895701608603 2206 8 0.8833712663638238 2206 9 1.2535653487128968 2206 10 1.065284224738619 2206 11 0.9756563011125579 2206 12 0.8785720273571513 2206 13 0.813535399522948 2206 14 1.1375785035399915 2206 15 0.9602876500631012 2206 16 0.9490990415259191 2206 17 0.9122951435167366 2206 18 1.0515950534628544 2206 19 1.0157100546309508 2206 20 1.0787794429337865 2206 21 1.2112986122478233 2206 22 1.006167634991769 2206 23 1.2274045435212813 2206 24 1.1624611316914368 2206 25 1.4479142021818632 2207 1 -0.7913015532947902 2207 2 0.02032789569675586 2207 3 0.3264863237350113 2207 4 0.4191514010872126 2207 5 0.33390359679280124 2207 6 0.4034027994483367 2207 7 0.5701314652274192 2207 8 0.6590166853144908 2207 9 0.8285047379063144 2207 10 0.6851263547307909 2207 11 0.5906902870170256 2207 12 0.4647277047259877 2207 13 0.5646671144798816 2207 14 -5.0825524283315815 2207 15 -1.6310332583159166 2207 16 -0.7271352576126873 2207 17 -0.41886538898130965 2207 18 -0.055957648181625075 2207 19 0.15854043834277576 2207 20 0.3350137420723982 2207 21 0.45562938750232007 2207 22 0.2405832966988939 2207 23 0.6740939718689702 2207 24 0.4902669265230699 2207 25 0.4146224245781056 2208 1 0.6728979945575091 2208 2 1.0610935324714355 2208 3 0.9997336485277764 2208 4 0.8671170378618953 2208 5 0.8276265809676318 2208 6 0.7463682855071099 2208 7 0.8593854215198107 2208 8 0.8903812670778635 2208 9 1.1136308871150575 2208 10 1.1956776734949202 2208 11 0.8699981996018025 2208 12 0.9982312465496506 2208 13 0.7823949215635295 2208 14 1.2202261700105481 2208 15 1.1029809089787364 2208 16 1.2358121425380233 2208 17 1.0036606095115346 2208 18 1.1651914098439988 2208 19 1.004241719427931 2208 20 0.847831225116856 2208 21 1.1046343611270402 2208 22 1.2510815634660375 2208 23 1.695207460942072 2208 24 1.442182525618776 2208 25 1.269448046281191 2209 1 -0.42167469006792757 2209 2 -0.2255295981607368 2209 3 0.3829167905654778 2209 4 0.4257027524385677 2209 5 0.5448447925340005 2209 6 0.4222149206604584 2209 7 0.5826785577745142 2209 8 0.6514478073456118 2209 9 0.6722190704206525 2209 10 0.07013601894045252 2209 11 0.3460685015952407 2209 12 0.23031460071288024 2209 13 0.4368623642751288 2209 14 0.7820058026266494 2209 15 0.765139059456402 2209 16 0.6774819414045083 2209 17 0.6541206880047667 2209 18 0.4341081994842213 2209 19 0.5325034195057583 2209 20 0.40854560120425276 2209 21 0.2563180033909376 2209 22 0.6429891935047891 2209 23 0.8277096966846997 2209 24 0.5063324465885941 2209 25 0.6823478955035753 2210 1 -0.3154425655111943 2210 2 -0.1609914760309934 2210 3 0.5574475802795149 2210 4 0.7829028845078057 2210 5 0.9817388498469057 2210 6 0.9702872671621208 2210 7 1.1367283238661656 2210 8 1.023308482871208 2210 9 1.523823411553785 2210 10 1.456537174975118 2210 11 1.1882093097046322 2210 12 1.1292666227015502 2210 13 0.9680444268708044 2210 14 1.260471655438053 2210 15 1.1672620296422025 2210 16 1.2166846376752645 2210 17 1.0963318186975783 2210 18 1.0576465403000554 2210 19 1.0862444523043848 2210 20 1.2230612115313804 2210 21 1.2512496318304187 2210 22 1.3037749197355453 2210 23 1.464150516371589 2210 24 1.2113978165754276 2210 25 1.3597644839149297 2211 1 -1.5104849586534783 2211 2 -0.5043600904743748 2211 3 0.15376998838496903 2211 4 0.37858649183915816 2211 5 0.6939609865940142 2211 6 0.5093001201052019 2211 7 0.5134676795748732 2211 8 0.46200095497740534 2211 9 0.9490392236452934 2211 10 0.9331519381361488 2211 11 0.6604444788925647 2211 12 1.0024348784511403 2211 13 0.8154162933908538 2211 14 1.1163025414177739 2211 15 0.6565283665760475 2211 16 0.7757853992585311 2211 17 0.6661579472330388 2211 18 0.7852323258892469 2211 19 0.7267941980662016 2211 20 0.8009262744838068 2211 21 0.9256497850822711 2211 22 1.049554083215746 2211 23 0.9594194688439046 2211 24 0.8367422938523745 2211 25 1.2313014219851937 2212 1 0.9293841609909244 2212 2 0.06928027664913827 2212 3 0.43799108563977285 2212 4 0.42482759156340677 2212 5 0.5443416920308977 2212 6 0.6543361327816655 2212 7 0.6272743223702761 2212 8 0.7913214472192465 2212 9 0.9997047379063133 2212 10 0.7868787356831675 2212 11 0.7181569536836889 2212 12 0.7705181809164614 2212 13 0.43373378114655026 2212 14 0.5872380478588966 2212 15 0.48736674168408456 2212 16 -0.10058287666031163 2212 17 0.31903937292345574 2212 18 0.39691854229456425 2212 19 0.3170547240570656 2212 20 0.5827470754057273 2212 21 0.7861436732166069 2212 22 0.9642785347941327 2212 23 1.027655876630881 2212 24 1.2100764503325934 2212 25 0.6599938531495297 2213 1 0.4386513409167847 2213 2 0.2393857799282948 2213 3 -0.4611591852470234 2213 4 -0.9396206548010113 2213 5 -1.864469257498616 2213 6 -0.4584782384758199 2213 7 -0.15657512159892661 2213 8 -0.20810826478531652 2213 9 -0.5425307910358015 2213 10 -0.5631355215167204 2213 11 -0.4703061201686083 2213 12 -0.459085468926709 2213 13 -0.46562749629855915 2213 14 -7.219411510167909 2213 15 -3.4568248750823827 2213 16 -2.0564653973332483 2213 17 -1.2322466265062602 2213 18 -4.320743276924137 2213 19 -2.787980918942651 2213 20 -5.028430569304849 2213 21 -1.8156584368489774 2213 22 -1.5172897571933177 2213 23 -4.468962315556176 2213 24 -2.0726153090058688 2213 25 -1.657839052467807 2214 1 0.32963589163912843 2214 2 0.343217763538167 2214 3 -1.5519753502737998 2214 4 -1.8650759116881162 2214 5 -0.3568435397710772 2214 6 -0.3939311212565144 2214 7 -0.5131724995302882 2214 8 -0.5663982926590805 2214 9 -5.153687332578265 2214 10 -1.577553821480663 2214 11 -0.778201342938921 2214 12 -1.1183154670801798 2214 13 -1.5618315639342129 2214 14 -3.018335688243475 2214 15 -2.1594614521485127 2214 16 -1.1393766673043189 2214 17 -1.3127332303909967 2214 18 -0.854086282542859 2214 19 -3.8880868744325525 2214 20 -1.7754761257690106 2214 21 -3.629394401043932 2214 22 -6.366151505063218 2214 23 -6.193361534738958 2214 24 -5.421222060261069 2214 25 -3.2291062097831293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22205.0 3 8.0 4 407.0 5 2739.0 6 3334.0 7 2639.0 8 1937.0 9 1655.0 10 1499.0 11 1417.0 12 1331.0 13 1270.0 14 1312.0 15 1333.0 16 1378.0 17 1447.0 18 1635.0 19 1976.0 20 2492.0 21 3322.0 22 4205.0 23 5181.0 24 6239.0 25 7200.0 26 8525.0 27 9450.0 28 11009.0 29 13102.0 30 16307.0 31 20488.0 32 26023.0 33 33787.0 34 44597.0 35 58387.0 36 78634.0 37 115607.0 38 186691.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26227372835119 18.354247779774163 8.703370972671728 30.68010751920292 2 14.53833032381469 20.662332008193506 37.54637394637109 27.252963721620716 3 18.963782193566068 23.311403477468964 28.31420834838067 29.410605980584297 4 12.564377804930968 16.343683266909522 35.65631102330734 35.435627904852176 5 13.703033382624136 37.904864945104514 34.512078149710824 13.880023522560533 6 33.808871686296534 36.59921556942841 16.53664448219847 13.055268262076591 7 29.68007674612445 31.04486531541256 20.36746067261516 18.90759726584783 8 26.4899232520102 37.03633805716638 18.73334362740052 17.74039506342289 9 26.398492413453894 15.430886078845173 18.713718694416325 39.45690281328461 10 14.712355278481882 29.6828971706515 33.12952433148364 22.47522321938298 11 35.92802578947443 22.473460615825022 22.506282545440534 19.092231049260008 12 23.284670491195023 24.717584268059483 30.524873704940493 21.472871535805005 13 29.39874537650121 21.304768482579114 25.035247043243984 24.261239097675695 14 21.225406055344052 21.420186955391745 26.490059605240234 30.864347384023965 15 23.649811488767813 30.075889684539796 22.279287738635812 23.99501108805658 16 23.076407143244484 28.02755920119416 26.101469005888013 22.794564649673347 17 22.38253031820919 28.22554635567848 26.721123850134287 22.670799475978047 18 22.36079002197677 27.553871621428776 27.96072723121272 22.12461112538174 19 23.787764840863588 26.86432127817156 26.41394732223001 22.93396655873484 20 23.858124451484468 27.030417192884816 26.987748927942008 22.123709427688706 21 23.351874948270893 26.33646609567245 26.51184560894792 23.799813347108735 22 23.361654641764463 27.386381798255627 26.276884789259785 22.975078770720124 23 22.95010616923147 27.113395588839673 26.822571806931823 23.11392643499703 24 23.41333660595665 26.639391634546918 27.26599435187075 22.68127740762569 25 23.18616601523759 26.818329059444867 26.937484213725817 23.058020711591727 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 112.0 1 112.0 2 126.5 3 141.0 4 141.0 5 141.0 6 223.0 7 305.0 8 305.0 9 305.0 10 527.5 11 750.0 12 750.0 13 750.0 14 1251.5 15 1753.0 16 1753.0 17 1753.0 18 3332.5 19 4912.0 20 4912.0 21 4912.0 22 8269.5 23 11627.0 24 11627.0 25 11627.0 26 17384.5 27 23142.0 28 23142.0 29 23142.0 30 31894.5 31 40647.0 32 40647.0 33 40647.0 34 49046.0 35 57445.0 36 57445.0 37 57445.0 38 65418.5 39 73392.0 40 73392.0 41 73392.0 42 80216.0 43 87040.0 44 87040.0 45 87040.0 46 92244.0 47 97448.0 48 97448.0 49 97448.0 50 96151.0 51 94854.0 52 94854.0 53 94854.0 54 85886.5 55 76919.0 56 76919.0 57 76919.0 58 67088.5 59 57258.0 60 57258.0 61 57258.0 62 47205.0 63 37152.0 64 37152.0 65 37152.0 66 28722.5 67 20293.0 68 20293.0 69 20293.0 70 14936.0 71 9579.0 72 9579.0 73 9579.0 74 6773.0 75 3967.0 76 3967.0 77 3967.0 78 2678.0 79 1389.0 80 1389.0 81 1389.0 82 935.5 83 482.0 84 482.0 85 482.0 86 307.5 87 133.0 88 133.0 89 133.0 90 78.5 91 24.0 92 24.0 93 24.0 94 14.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08761815608018632 2 0.03039522352618841 3 0.028682816566966526 4 0.030823325265993882 5 0.023402895109365725 6 0.3827229553860907 7 0.1882220649344719 8 0.19064797479336956 9 0.1957851956710352 10 0.015982464952737568 11 0.0024259098588976665 12 0.003995616238184392 13 0.0 14 0.07049408648796748 15 0.001997808119092196 16 0.003139412758573451 17 0.004851819717795333 18 0.003995616238184392 19 0.0024259098588976665 20 0.001855107539157039 21 2.8540115987031367E-4 22 0.0 23 0.0 24 7.135028996757843E-4 25 1.4270057993515684E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 700768.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.17804986744105 #Duplication Level Percentage of deduplicated Percentage of total 1 91.45175558863201 77.89682197993528 2 6.400614217061642 10.903836739262571 3 1.1527036727902449 2.9455515276992976 4 0.3955025752637669 1.3475255231407397 5 0.2013142144488389 0.8573776098673961 6 0.11188956093189945 0.571832076042205 7 0.061013728469642925 0.3637921284330027 8 0.04397544304383609 0.29965939844245465 9 0.030167216872635093 0.23126262328252983 >10 0.1330829445458379 1.959660629989896 >50 0.00907459860437453 0.5652556570663704 >100 0.007393859077507518 1.1818666253070458 >500 0.0015123802577126683 0.8755574815311844 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 851 0.1214381935248185 No Hit CTGTAGGACGTGGAATATGGCAAGA 806 0.11501666742773642 No Hit GTCCTACAGTGGACATTTCTAAATT 794 0.11330426046851454 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.4270057993515686E-4 0.0 0.0 0.0 6 0.0 1.4270057993515686E-4 0.0 0.0 0.0 7 0.0 1.4270057993515686E-4 0.0 0.0 0.0 8 0.0 1.4270057993515686E-4 0.0 0.0 0.0 9 0.0 1.4270057993515686E-4 0.0 0.0 0.0 10 1.4270057993515686E-4 1.4270057993515686E-4 0.0 0.0 0.0 11 1.4270057993515686E-4 1.4270057993515686E-4 0.0 0.0 0.0 12 1.4270057993515686E-4 1.4270057993515686E-4 0.0 0.0 0.0 13 1.4270057993515686E-4 2.854011598703137E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 160 0.0 14.295802 4 GTAAGAC 75 9.307696E-7 13.978117 3 AAGGCGT 70 7.022869E-6 13.613095 6 CTAGGAC 140 3.6379788E-12 12.934297 3 TAGAGTG 90 5.184884E-7 12.707379 5 AGGCGTG 60 4.0703034E-4 12.672822 7 GTCCTAC 415 0.0 12.620867 1 TAGTATA 55 0.002991115 12.130641 2 TTTAGAC 55 0.0029927236 12.12977 3 CCCCTAT 55 0.0030104688 12.120204 1 AAAGGCG 80 2.7686116E-5 11.913168 5 AGGACGT 385 0.0 11.882225 5 CTCTTAT 65 7.852641E-4 11.720637 1 GGACGTG 385 0.0 11.63301 6 TGTAGGA 460 0.0 11.603223 2 GACGTGG 230 0.0 11.570837 7 TAGGACG 430 0.0 11.525297 4 GTAGGAC 455 0.0 11.310964 3 GGCGAGG 135 4.8839865E-9 11.232574 19 GTTTATA 60 0.005765359 11.110188 1 >>END_MODULE