FastQCFastQC Report
Thu 26 May 2016
SRR1926450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726893
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA501.12006295E-418.7009471
TGTAGGA4300.018.1494372
CTGTAGG4200.016.220211
CTCCGGA500.00240866416.0293831
TAGGACC1550.015.2278134
GTAGGAC4200.015.119283
GTGTAGC1456.548362E-1114.7396621
GATTACA550.004543517714.5721661
GTCCTAT751.5067407E-414.2483411
TAGGACA1700.013.8841814
TCTATAC652.4206984E-413.4750073
TAGGACG3750.013.4466194
ATGTAGG600.00808699413.357821
GTCTTAC600.00808699413.357821
GTCCTAA2000.013.3578191
ATAGGAC500.00902695113.1381333
CTAAAGT2300.013.0610084
GTCCTAC5700.012.8891231
GTTACAC851.24069575E-512.8805223
TCCTACA6300.012.8223692
Produced by FastQC (version 0.11.5)