FastQCFastQC Report
Thu 26 May 2016
SRR1926450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726893
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14060.19342599254635828No Hit
CTGTAGGACGTGGAATATGGCAAGA12650.17402836455984583No Hit
GTCCTACAGTGTGCATTTCTCATTT10270.14128626909325032No Hit
CTTTAGGACGTGAAATATGGCGAGG9860.13564582407589562No Hit
CCCATGTACTCTGCGTTGATACCAC9460.13014295088823252No Hit
GTACATGGGAAGCAGTGGTATCAAC9400.12931751991008306No Hit
GTATCAACGCAGAGTACTTTTTTTT9370.12890480442100832No Hit
GTCCTAAAGTGTGTATTTCTCATTT8900.12243892842550416No Hit
CTGTAGGACCTGGAATATGGCGAGA7960.10950717643449587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG350.002173120416.2807469
TGCGCCA601.50085E-615.7958112
CCAATAC508.353483E-515.2733433
CTCGAAC456.922033E-414.73058418
CATGCGC400.005356766314.21720710
GATATAC1750.014.1735441
GGCGTGC551.9629541E-413.8139678
CACGTTT552.0117353E-413.77405318
GTTACAC801.8901883E-613.1255283
TAGGACC2950.012.9426144
ACTGTTC2500.012.9160598
GCGCCAC751.5124617E-512.63664813
CGCCACC905.5490455E-712.63664714
GTGCGCC604.1724104E-412.63577811
GCCTCGA604.1918366E-412.62882116
TCCAACG604.1991417E-412.62621618
GTATTAG550.00297099612.1416711
GCCGGTT550.003122310212.06142311
ACGTTTT550.00314168112.05146719
GTCCTAC6650.011.9069241
Produced by FastQC (version 0.11.5)