##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926450_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 726893 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.532536425581206 31.0 28.0 31.0 23.0 34.0 2 28.967500030953662 31.0 28.0 33.0 23.0 34.0 3 29.32819410834882 31.0 30.0 33.0 25.0 34.0 4 33.10866110968189 35.0 33.0 37.0 28.0 37.0 5 32.80729625955952 35.0 33.0 37.0 26.0 37.0 6 32.902290983679855 35.0 33.0 37.0 26.0 37.0 7 32.741480520516774 35.0 33.0 37.0 25.0 37.0 8 32.70229593626572 35.0 33.0 37.0 25.0 37.0 9 33.96681905039669 38.0 34.0 39.0 25.0 39.0 10 33.874442318195385 37.0 34.0 39.0 25.0 39.0 11 34.092141484372526 38.0 34.0 39.0 26.0 39.0 12 33.88332808267516 37.0 34.0 39.0 25.0 39.0 13 34.0228369237288 38.0 34.0 39.0 26.0 39.0 14 34.7075250415123 39.0 34.0 40.0 24.0 41.0 15 34.84494279075462 39.0 34.0 40.0 25.0 41.0 16 34.82918806481835 39.0 34.0 40.0 25.0 41.0 17 34.7569036983435 39.0 34.0 40.0 25.0 41.0 18 34.71902191931962 39.0 34.0 40.0 24.0 41.0 19 34.7575695459992 39.0 34.0 41.0 24.0 41.0 20 34.74470382848645 39.0 34.0 41.0 24.0 41.0 21 34.66497407458869 39.0 34.0 41.0 23.0 41.0 22 34.599345433234326 39.0 34.0 41.0 22.0 41.0 23 34.261453886610546 39.0 34.0 40.0 18.0 41.0 24 34.34384015253964 39.0 34.0 41.0 18.0 41.0 25 34.13778231459101 39.0 34.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9240929176668295 1101 2 0.769120414086462 1101 3 0.8626144080070937 1101 4 0.6464177232071222 1101 5 0.6174563005410931 1101 6 0.21694141449610527 1101 7 0.5304229102873705 1101 8 0.7472578110650403 1101 9 1.0866908428186761 1101 10 0.9911480944168574 1101 11 0.6641095416927527 1101 12 0.8802196209143816 1101 13 0.7501657260294081 1101 14 1.1214470942238677 1101 15 0.9995717005521811 1101 16 0.8562836451148073 1101 17 0.906337017343418 1101 18 0.9682407944751716 1101 19 0.8639734600021001 1101 20 1.082824952446316 1101 21 1.0524464343588065 1101 22 1.0479246364189976 1101 23 1.3040167771068667 1101 24 1.0274940986161525 1101 25 1.1571506426270375 1102 1 0.7011672755658473 1102 2 0.8206981842390917 1102 3 0.7869900990361103 1102 4 0.45811088726217974 1102 5 0.49292556484142125 1102 6 0.24415937934417542 1102 7 0.5339533536897108 1102 8 0.7194997315855858 1102 9 0.7259552702336265 1102 10 1.0910540509281006 1102 11 0.8042051102110577 1102 12 0.773627045266565 1102 13 0.8108091614126351 1102 14 1.0664939253395573 1102 15 0.9864056121258855 1102 16 1.1268874297298055 1102 17 1.1165991953397807 1102 18 1.0160148867648715 1102 19 1.1034469435508427 1102 20 0.9485524550833446 1102 21 0.9017595356334738 1102 22 0.996195634169581 1102 23 1.2786777757470489 1102 24 1.0999596901369912 1102 25 1.1641842059856558 1103 1 0.5394135525874653 1103 2 0.4337172394832898 1103 3 0.41413821753090474 1103 4 0.13289391368331138 1103 5 0.4415007449855324 1103 6 0.22806522401991458 1103 7 0.31457529123974837 1103 8 0.35360701741424805 1103 9 0.7894844936123278 1103 10 0.6759353960041565 1103 11 0.7163127162959242 1103 12 0.7155402558350232 1103 13 0.5461974720611522 1103 14 0.9523296339064089 1103 15 0.6681939227743996 1103 16 0.841363010194172 1103 17 0.9295497157561172 1103 18 0.9210915881259609 1103 19 0.8892115552401947 1103 20 0.863256698478061 1103 21 0.950186116898486 1103 22 0.7733722554666116 1103 23 0.9479088405989344 1103 24 0.8700464795685363 1103 25 0.8199760394524276 1104 1 0.3617025627199659 1104 2 0.51384693550823 1104 3 0.4142660429401026 1104 4 0.13954395265447772 1104 5 0.2968578767080601 1104 6 -0.2195986886846839 1104 7 0.2872131712085704 1104 8 0.3450333619193131 1104 9 0.8311711654751477 1104 10 0.7850970483814024 1104 11 0.49677974669342717 1104 12 0.6296764990150692 1104 13 0.37511407377588313 1104 14 0.6362660329711005 1104 15 0.5639607193449407 1104 16 0.4029686220414064 1104 17 0.30552913898292644 1104 18 0.46087833793110633 1104 19 0.6490400821302984 1104 20 0.6024342510891287 1104 21 0.7116813624168046 1104 22 0.3898093560122149 1104 23 0.6881245849481132 1104 24 0.6679164717743049 1104 25 0.7597827424921846 1105 1 0.792032420511994 1105 2 0.6794228998606471 1105 3 0.4666350728768158 1105 4 0.470698945129854 1105 5 0.5436617512748398 1105 6 0.2396727711897313 1105 7 0.08496019690012702 1105 8 0.27734915577902797 1105 9 0.6539939275745894 1105 10 0.8877039494632797 1105 11 0.25396428862296716 1105 12 0.5055490608664641 1105 13 0.4455685412435386 1105 14 0.7408466150384854 1105 15 0.625095809566858 1105 16 0.7103567208859971 1105 17 0.6742465711020316 1105 18 0.49082240573602576 1105 19 0.7235197313408221 1105 20 0.6409636167170589 1105 21 0.707784859036849 1105 22 0.7641420667873717 1105 23 1.0887352556932726 1105 24 0.7823458506377179 1105 25 1.0470578004587168 1106 1 0.5922172850913547 1106 2 0.5299760264195257 1106 3 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0.8059149099800251 1107 15 0.5806896282958718 1107 16 0.5810255868812888 1107 17 0.6122650531794278 1107 18 0.5827504838314823 1107 19 0.5975551135224038 1107 20 0.6670468825271456 1107 21 0.6681149512543172 1107 22 0.538291273871522 1107 23 0.7829996381449433 1107 24 0.541268565458104 1107 25 0.7805097817837208 1108 1 1.3024083298532112 1108 2 1.1075740751207697 1108 3 1.0017081100040244 1108 4 0.8746819321164949 1108 5 -0.025426443954557953 1108 6 0.4743914913010201 1108 7 0.8851080101337558 1108 8 1.1247283691807581 1108 9 1.5337978576676292 1108 10 1.4116294052207508 1108 11 1.162983069598539 1108 12 1.0291955553741872 1108 13 0.9555676717539328 1108 14 1.5178116615561805 1108 15 1.1511334005009743 1108 16 1.0047731484430216 1108 17 1.3224148463244703 1108 18 1.3511388999539236 1108 19 1.4551408947179212 1108 20 1.4071977123029455 1108 21 1.5302242121365808 1108 22 1.606757201703175 1108 23 1.782946628617367 1108 24 1.681564759138432 1108 25 1.455435332847209 1109 1 0.6049151919317275 1109 2 -0.2472860392052354 1109 3 0.7680398568751663 1109 4 0.8606644054865953 1109 5 -0.6971156484570855 1109 6 0.3592521092658174 1109 7 0.8343261109118814 1109 8 1.0003283288896583 1109 9 1.1342058050877384 1109 10 -1.0162809974384643 1109 11 0.4796963228533002 1109 12 0.9044845181301042 1109 13 0.9781646851759049 1109 14 1.3021132404637825 1109 15 1.255400480151451 1109 16 1.0238220265876166 1109 17 1.3358972567397203 1109 18 1.1291047028800634 1109 19 1.0903590962238 1109 20 1.3845944033960933 1109 21 1.150559887390287 1109 22 1.2210706161223541 1109 23 1.0683285127300834 1109 24 1.1986104139947642 1109 25 1.4211891542065302 1110 1 0.6227422284607371 1110 2 -0.39370371908697877 1110 3 0.29484333532131757 1110 4 0.6290141411406651 1110 5 1.0486188603389053 1110 6 0.7568470274317747 1110 7 0.8572729354314603 1110 8 0.927124482889468 1110 9 1.1244869306553795 1110 10 0.7540676462072469 1110 11 0.7250652751911346 1110 12 0.9287470795555706 1110 13 0.7675622161716333 1110 14 1.0784326395928403 1110 15 1.0065217862999916 1110 16 0.7030851872859714 1110 17 1.1723370431322309 1110 18 1.1230613687920865 1110 19 1.287278167750351 1110 20 1.1533895985593006 1110 21 1.1766595531291912 1110 22 1.1444702245974057 1110 23 1.4202708227272822 1110 24 1.3591369751006255 1110 25 1.4848046340909349 1111 1 0.18323230258746648 1111 2 0.26639223948328805 1111 3 0.564325717530906 1111 4 0.27361266368331627 1111 5 0.5408069949855374 1111 6 -0.050097275980085954 1111 7 0.31215654123975156 1111 8 0.2567320174142509 1111 9 0.6406094936123239 1111 10 0.57164164600416 1111 11 0.30980646629592457 1111 12 0.3315465058350213 1111 13 0.2454162220611522 1111 14 0.5361296339064054 1111 15 0.5030439227744026 1111 16 0.40026926019417175 1111 17 0.40787471575611534 1111 18 0.42517283812596673 1111 19 0.26311780524019923 1111 20 0.19135669847806014 1111 21 0.3904548668984873 1111 22 0.3083785054666137 1111 23 0.7534463405989342 1111 24 0.6693839795685363 1111 25 0.7313197894524279 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0.8644732963856683 2202 22 0.8443761016204618 2202 23 1.195713968804064 2202 24 0.7901772487993028 2202 25 0.8711234753498687 2203 1 -0.11536455686526992 2203 2 0.1608522311914129 2203 3 -0.13938748727838401 2203 4 -0.6360745771956218 2203 5 -0.45559510907748546 2203 6 -0.6007321225804176 2203 7 -0.3146611930056906 2203 8 -0.38022382835689683 2203 9 -0.08556691600625044 2203 10 -0.0021236421384571713 2203 11 -0.0762126568384005 2203 12 -0.2021897607487304 2203 13 -0.3418622294313849 2203 14 -0.005371195280986285 2203 15 -0.24006345699342546 2203 16 -0.1899139383962094 2203 17 -0.1106731698160246 2203 18 -0.14474191104484646 2203 19 -0.04650983779463047 2203 20 -0.09402887366123736 2203 21 -0.09901056635192873 2203 22 -0.11097135979043316 2203 23 0.13342923860888334 2203 24 0.026854439767539873 2203 25 -0.033834905821201744 2204 1 0.09195194121305761 2204 2 0.3180559440646462 2204 3 -0.012458938867201397 2204 4 -0.10891967241463618 2204 5 0.08077910201554772 2204 6 -0.03309243790741334 2204 7 0.24818413800119998 2204 8 0.1261188657554797 2204 9 0.36892209234850526 2204 10 0.4320718888951518 2204 11 0.3060112944949793 2204 12 0.29942145646693064 2204 13 0.04540758264567302 2204 14 0.5467536465446372 2204 15 0.4097876036590762 2204 16 0.4291039264658991 2204 17 0.2715743603058769 2204 18 0.42508274136451263 2204 19 0.4047881745134987 2204 20 0.3967871882095011 2204 21 0.5037064960454032 2204 22 0.6541760469358096 2204 23 0.7290412260649646 2204 24 0.7329330514484766 2204 25 0.47743575509224456 2205 1 0.6105883557370717 2205 2 0.669320389089588 2205 3 0.6985161702868083 2205 4 0.6061852522659876 2205 5 0.5473338158516796 2205 6 0.19654396417739406 2205 7 0.34995796840509996 2205 8 0.4433707969418066 2205 9 1.064256147155632 2205 10 0.8462787030907748 2205 11 0.7087914564534046 2205 12 0.6383686022917132 2205 13 0.6729691256044603 2205 14 1.1650666417804274 2205 15 1.0005340802547167 2205 16 0.93947324641465 2205 17 1.0638048338663495 2205 18 1.1099230841889565 2205 19 0.9698414765000365 2205 20 0.9965732339111355 2205 21 0.9381357231976963 2205 22 0.6748462712146477 2205 23 0.8647529350871253 2205 24 0.7596275819307436 2205 25 1.0866532048067583 2206 1 0.037086390220242293 2206 2 0.3949724746881422 2206 3 0.485415911005866 2206 4 0.2032065085239836 2206 5 0.3871662988550355 2206 6 0.28200391901233246 2206 7 0.3857303746995342 2206 8 0.4810728747738864 2206 9 0.8582735679674087 2206 10 0.9218704491149339 2206 11 0.6132195448239983 2206 12 0.6499657490064905 2206 13 0.4354235722128976 2206 14 0.7958792545437348 2206 15 0.7832506754299402 2206 16 0.8275845579938732 2206 17 0.9511837066514133 2206 18 0.754152589643418 2206 19 0.7614118587303338 2206 20 0.856170810769413 2206 21 0.9091894552899902 2206 22 0.8880648199582666 2206 23 1.0018790985655457 2206 24 0.6378850228158797 2206 25 0.5800003186633589 2207 1 -1.0102323232962753 2207 2 -0.05630758376887357 2207 3 0.03871323716955288 2207 4 0.3108137879959614 2207 5 -0.10938755037974346 2207 6 -0.05028261572871173 2207 7 0.20768322525388783 2207 8 0.24991495142838716 2207 9 0.5326141086948084 2207 10 0.30192628367108654 2207 11 0.08325317191258108 2207 12 0.11879304452315864 2207 13 -0.10069962141881916 2207 14 -5.554031403014253 2207 15 -2.3375147967856904 2207 16 -1.0276550573627787 2207 17 -0.8036556259563739 2207 18 -0.39616025790702736 2207 19 -0.12755199542751683 2207 20 -0.23987857253528944 2207 21 -0.008061801404736002 2207 22 -0.13789812945090318 2207 23 0.13644049810089598 2207 24 -0.1532974324503158 2207 25 -0.07167203595997762 2208 1 0.20391610131310145 2208 2 0.6829730115972303 2208 3 0.5819583074859267 2208 4 0.3986630291255935 2208 5 0.6459337284937803 2208 6 0.5515027052792902 2208 7 0.8503558009848788 2208 8 0.7389143637410811 2208 9 1.1434814951115797 2208 10 1.0757938667687768 2208 11 0.8420110671205094 2208 12 0.755095278323779 2208 13 0.7336037689127295 2208 14 0.9113545214626271 2208 15 0.8867114640037883 2208 16 0.8885294269857766 2208 17 0.5378831490394731 2208 18 0.7104673002698902 2208 19 0.4688817201577393 2208 20 0.5627397569488295 2208 21 0.8300385906616015 2208 22 0.670870306441131 2208 23 1.0986182858762916 2208 24 0.879361022297175 2208 25 0.9669445551945586 2209 1 -0.3085046955877182 2209 2 -0.1990756234526394 2209 3 0.366096043970483 2209 4 0.5800407101147798 2209 5 0.5598326184648528 2209 6 0.5765868785211339 2209 7 0.802048446146479 2209 8 0.8459022323372025 2209 9 0.9606799518442841 2209 10 0.6396129304729357 2209 11 0.7570419944792164 2209 12 0.9599496637831137 2209 13 0.987803311477208 2209 14 1.1226868115219801 2209 15 1.035257020909036 2209 16 1.0137393281179357 2209 17 1.003046552739086 2209 18 1.0009832345168803 2209 19 0.9823664619717434 2209 20 0.9322630245121246 2209 21 1.0829990933786178 2209 22 0.8643769594406621 2209 23 1.0356759127806043 2209 24 1.0498901129829719 2209 25 0.9704805001174748 2210 1 -0.3834234222024513 2210 2 -0.5668272983318374 2210 3 0.23390292341325747 2210 4 0.3612636615824698 2210 5 0.8102200727166249 2210 6 0.4625996778014283 2210 7 0.9147366357775653 2210 8 0.9443633199352561 2210 9 1.1593332331081285 2210 10 1.1960967405419751 2210 11 0.8689648171362592 2210 12 0.8483335331459472 2210 13 0.8024999930695529 2210 14 1.1014657683601925 2210 15 0.8612897210937334 2210 16 0.9163209933874512 2210 17 0.8946656821426728 2210 18 0.7131520923276469 2210 19 0.9108922275091018 2210 20 0.8969239253688173 2210 21 1.2358264530329421 2210 22 1.1165520873993913 2210 23 1.4175088405989342 2210 24 1.0311355551987873 2210 25 1.0821272999566318 2211 1 -1.4015963053908145 2211 2 -0.462709159848373 2211 3 0.379615243428983 2211 4 0.624446127551316 2211 5 0.8994529588535372 2211 6 0.8014861095671151 2211 7 0.9112082068621348 2211 8 0.8540247283582758 2211 9 1.1408261811645417 2211 10 1.1359267076165267 2211 11 0.9220042785348568 2211 12 1.0364852850747894 2211 13 0.9768574553527145 2211 14 1.299308414190449 2211 15 0.9644839812539345 2211 16 1.1319728681724541 2211 17 1.0056686798329721 2211 18 1.0678230835311453 2211 19 1.1947587565768742 2211 20 1.0147380685699616 2211 21 1.2695725830638978 2211 22 1.1845996573045454 2211 23 1.6136658999138902 2211 24 1.5396912581817332 2211 25 1.6466660979319627 2212 1 0.9297032077598786 2212 2 0.0608965498281151 2212 3 0.48679338994469745 2212 4 0.461893913683312 2212 5 0.5386248829165723 2212 6 0.4161203964337119 2212 7 0.413099429170785 2212 8 0.603607017414248 2212 9 0.6452431143019837 2212 10 0.33928712014208884 2212 11 0.1964713369855815 2212 12 0.43669197997294873 2212 13 0.305680230681844 2212 14 0.2809572201133079 2212 15 0.4140353020847485 2212 16 -0.1398438863575464 2212 17 0.5030255778250847 2212 18 0.5230984846776892 2212 19 0.42128052075743483 2212 20 0.4012704915815135 2212 21 0.6367102548295236 2212 22 0.3290412209838536 2212 23 0.782164013012725 2212 24 0.6274119968099185 2212 25 0.1358725911765717 2213 1 0.4733645580719248 2213 2 0.3074971297940756 2213 3 -0.41954368374880247 2213 4 -1.3702358852197953 2213 5 -1.8347171709194008 2213 6 -0.34245616537679524 2213 7 -0.3714400652501979 2213 8 -0.15187744328044772 2213 9 -0.41370380620485747 2213 10 -0.5677764869940134 2213 11 -0.5521465158795777 2213 12 -0.4326659598871032 2213 13 -0.5278427473172727 2213 14 -7.529181152199623 2213 15 -3.210564395324319 2213 16 -2.074797867319539 2213 17 -1.3305336480464405 2213 18 -4.9251285215632485 2213 19 -2.8955416440285404 2213 20 -4.947194489821754 2213 21 -2.125342950743196 2213 22 -1.7862270132719615 2213 23 -4.221796461046406 2213 24 -2.1178789317660147 2213 25 -1.945201291443361 2214 1 0.6707025330200089 2214 2 0.5929724403071823 2214 3 -1.1738453046112163 2214 4 -1.533754901970653 2214 5 0.1274517233583481 2214 6 0.027598076749065115 2214 7 -0.048047983734505806 2214 8 0.034348521018777944 2214 9 -4.711125187129177 2214 10 -0.9092213496189103 2214 11 -0.3352316709337373 2214 12 -0.5310492395305815 2214 13 -0.8497860500809722 2214 14 -1.6913935380812362 2214 15 -1.356990835207057 2214 16 -0.707576227704898 2214 17 -1.1967950834199925 2214 18 -0.6587057342221314 2214 19 -3.8998512665929645 2214 20 -1.6968899544570561 2214 21 -3.07273787898205 2214 22 -5.160216210032871 2214 23 -5.3122542901940655 2214 24 -4.529452593551959 2214 25 -2.3277438369636343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22297.0 3 15.0 4 423.0 5 2738.0 6 3371.0 7 2690.0 8 2057.0 9 1690.0 10 1490.0 11 1339.0 12 1342.0 13 1328.0 14 1287.0 15 1282.0 16 1346.0 17 1387.0 18 1726.0 19 1926.0 20 2565.0 21 3276.0 22 4242.0 23 5176.0 24 6237.0 25 7319.0 26 8340.0 27 9431.0 28 10968.0 29 13254.0 30 16392.0 31 20623.0 32 26397.0 33 34587.0 34 45144.0 35 59504.0 36 81246.0 37 120757.0 38 201701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.651876136828356 18.988561837266076 8.94178750380377 30.417774522101805 2 13.685405628569464 21.148558453175532 38.91887428610748 26.247161632147524 3 18.407033855037522 23.986695235854054 28.882264947561232 28.724005961547196 4 12.042814246828701 17.049598582032658 37.03958033451038 33.868006836628254 5 13.011117119869219 38.38977208459414 35.27893251430749 13.320178281229145 6 32.536268410404425 37.91441967090863 16.984180689531357 12.56513122915559 7 28.671530150012547 31.488435441669903 21.188548206108027 18.651486202209522 8 25.227550342325056 38.24803469849752 19.539412188040266 16.985002771137157 9 26.10106162967048 15.509444367847788 19.531090583206947 38.85840341927479 10 14.303444423193614 30.148369014088384 33.99622163300856 21.55196492970944 11 34.95878922452753 22.751842481423854 23.622420973127355 18.666947320921263 12 23.13069486463024 25.052520358087722 31.375187277897698 20.44159749938434 13 29.092589968537325 22.040520406717356 25.316380815333204 23.550508809412115 14 20.431729948511798 22.543709903907047 27.124645502354138 29.899914645227017 15 22.7150160412505 31.63180514970917 22.660261172469582 22.992917636570752 16 22.00624319858682 28.783511792326287 27.57036668129558 21.639878327791315 17 21.526959948050234 29.140572548070043 27.66476441000693 21.667703093872788 18 21.342378645652047 28.250045744773054 29.367456411766245 21.040119197808654 19 23.23034020886615 27.481816043154485 27.0016797890152 22.286163958964174 20 23.185712143494897 27.889082562117974 27.838042711137835 21.0871625832493 21 22.450209315538878 27.151726595248544 27.04607143004541 23.351992659167166 22 22.646937031997833 28.594304801394426 26.947845143645626 21.810913022962115 23 21.87749778853284 28.23551746955879 27.539954298638175 22.347030443270192 24 22.778487037964375 27.371741606731128 28.14104433671027 21.708727018594235 25 22.332722972982268 27.71191908575265 27.803404352497544 22.151953588767533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 149.0 1 149.0 2 156.5 3 164.0 4 164.0 5 164.0 6 283.0 7 402.0 8 402.0 9 402.0 10 681.5 11 961.0 12 961.0 13 961.0 14 1659.0 15 2357.0 16 2357.0 17 2357.0 18 4447.5 19 6538.0 20 6538.0 21 6538.0 22 10812.5 23 15087.0 24 15087.0 25 15087.0 26 22390.5 27 29694.0 28 29694.0 29 29694.0 30 40401.5 31 51109.0 32 51109.0 33 51109.0 34 60049.0 35 68989.0 36 68989.0 37 68989.0 38 76309.5 39 83630.0 40 83630.0 41 83630.0 42 89291.0 43 94952.0 44 94952.0 45 94952.0 46 98315.5 47 101679.0 48 101679.0 49 101679.0 50 97095.0 51 92511.0 52 92511.0 53 92511.0 54 81354.5 55 70198.0 56 70198.0 57 70198.0 58 60066.5 59 49935.0 60 49935.0 61 49935.0 62 40203.5 63 30472.0 64 30472.0 65 30472.0 66 23455.5 67 16439.0 68 16439.0 69 16439.0 70 11901.0 71 7363.0 72 7363.0 73 7363.0 74 5091.5 75 2820.0 76 2820.0 77 2820.0 78 1929.5 79 1039.0 80 1039.0 81 1039.0 82 659.5 83 280.0 84 280.0 85 280.0 86 190.0 87 100.0 88 100.0 89 100.0 90 61.5 91 23.0 92 23.0 93 23.0 94 12.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08804597100260973 2 0.03342995461505339 3 0.03246695180721234 4 0.03012823070245552 5 0.024212642025717677 6 0.3821745428832029 7 0.20456931075137608 8 0.21447448248916967 9 0.21915192469868333 10 0.01705890688175564 11 0.002751436593831554 12 0.004402298550130487 13 0.0 14 0.07057434863177936 15 0.002338721104756821 16 0.0030265802532147097 17 0.005090157698588375 18 0.004677442209513642 19 0.0022011492750652435 20 0.001375718296915777 21 0.0 22 0.0 23 0.0 24 1.3757182969157772E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 726893.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.03905375391999 #Duplication Level Percentage of deduplicated Percentage of total 1 91.72255507199041 77.08276736141889 2 6.301806443713737 10.591957009401161 3 1.016807195444731 2.5635454366605717 4 0.3497395370646298 1.1756711902098207 5 0.17242315258263766 0.7245139294156316 6 0.09760648551917084 0.4921654009966086 7 0.07417981848396292 0.43638012271208504 8 0.04073955417537923 0.27389708666043444 9 0.036205817874115434 0.2738432407074681 >10 0.16215583984244494 2.4548086855409226 >50 0.013629922274703922 0.799730157814175 >100 0.01050911225344386 1.7884310906700138 >500 0.0011494341464415435 0.8319792449984493 >1k 4.92614634189233E-4 0.5103100427938738 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1406 0.19342599254635828 No Hit CTGTAGGACGTGGAATATGGCAAGA 1265 0.17402836455984583 No Hit GTCCTACAGTGTGCATTTCTCATTT 1027 0.14128626909325032 No Hit CTTTAGGACGTGAAATATGGCGAGG 986 0.13564582407589562 No Hit CCCATGTACTCTGCGTTGATACCAC 946 0.13014295088823252 No Hit GTACATGGGAAGCAGTGGTATCAAC 940 0.12931751991008306 No Hit GTATCAACGCAGAGTACTTTTTTTT 937 0.12890480442100832 No Hit GTCCTAAAGTGTGTATTTCTCATTT 890 0.12243892842550416 No Hit CTGTAGGACCTGGAATATGGCGAGA 796 0.10950717643449587 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACCG 35 0.0021731204 16.280746 9 TGCGCCA 60 1.50085E-6 15.79581 12 CCAATAC 50 8.353483E-5 15.273343 3 CTCGAAC 45 6.922033E-4 14.730584 18 CATGCGC 40 0.0053567663 14.217207 10 GATATAC 175 0.0 14.173544 1 GGCGTGC 55 1.9629541E-4 13.813967 8 CACGTTT 55 2.0117353E-4 13.774053 18 GTTACAC 80 1.8901883E-6 13.125528 3 TAGGACC 295 0.0 12.942614 4 ACTGTTC 250 0.0 12.916059 8 GCGCCAC 75 1.5124617E-5 12.636648 13 CGCCACC 90 5.5490455E-7 12.636647 14 GTGCGCC 60 4.1724104E-4 12.635778 11 GCCTCGA 60 4.1918366E-4 12.628821 16 TCCAACG 60 4.1991417E-4 12.626216 18 GTATTAG 55 0.002970996 12.141671 1 GCCGGTT 55 0.0031223102 12.061423 11 ACGTTTT 55 0.003141681 12.051467 19 GTCCTAC 665 0.0 11.906924 1 >>END_MODULE