FastQCFastQC Report
Thu 26 May 2016
SRR1926449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603261
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT6950.11520718229754616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA5100.015.4754762
TAGGACC2250.015.1892434
CTATAGT651.8045932E-514.7876064
GTATTAA550.004686734614.5032831
CCCAGGA1252.4045221E-814.1520562
CTGTAGG4700.014.1432711
GAACCAC450.006475656313.8143896
CTATACC703.6295627E-513.7313484
TCCTAGA903.9468032E-513.6077462
TGTAGAA1704.1836756E-1113.6077462
GTCTATG600.0083406713.2946751
GATCTAC600.0083406713.2946751
GTAGGAC5250.013.2723653
CTTAGAG755.7908863E-513.0649833
GTCCTAC4000.012.9623091
CCAGGAC1451.2369128E-1012.764643
TCTATAC600.002115275212.7020663
AGGACGT4800.012.5263535
CTTACAC704.742945E-412.44284253
GGCGAGG1950.012.2656719
Produced by FastQC (version 0.11.5)