##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926449_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 603261 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.581494576974144 27.0 10.0 31.0 2.0 31.0 2 16.60556044564459 20.0 2.0 30.0 2.0 31.0 3 20.20907202686731 25.0 15.0 31.0 2.0 31.0 4 22.55152910597569 28.0 7.0 35.0 2.0 37.0 5 23.15229394905356 30.0 7.0 35.0 2.0 37.0 6 23.92448210641828 31.0 7.0 35.0 2.0 37.0 7 23.657779965885414 31.0 8.0 35.0 2.0 37.0 8 23.784985271714895 31.0 10.0 35.0 2.0 37.0 9 26.557430365961 32.0 17.0 37.0 2.0 39.0 10 27.28012584934216 32.0 24.0 37.0 2.0 39.0 11 24.368999487783896 30.0 8.0 37.0 2.0 39.0 12 25.484586273602968 31.0 16.0 37.0 2.0 39.0 13 22.12024646048725 28.0 2.0 37.0 2.0 39.0 14 25.508297403611373 31.0 17.0 38.0 2.0 40.0 15 26.610458822963857 31.0 17.0 38.0 2.0 40.0 16 26.991492902740273 32.0 18.0 39.0 2.0 40.0 17 26.27349190483058 31.0 16.0 38.0 2.0 40.0 18 26.3487843570196 31.0 17.0 38.0 2.0 40.0 19 26.755946431146718 31.0 18.0 39.0 2.0 40.0 20 27.00825679100754 31.0 19.0 39.0 2.0 40.0 21 24.830229370040495 30.0 8.0 38.0 2.0 40.0 22 25.78119255181422 31.0 10.0 38.0 2.0 40.0 23 25.770029887561105 31.0 2.0 38.0 2.0 40.0 24 24.85312493265767 31.0 2.0 39.0 2.0 40.0 25 24.648323030993218 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.043747572481752 1101 2 10.845232726295984 1101 3 7.27289961351455 1101 4 8.698322049593962 1101 5 8.318363942486677 1101 6 7.960242826752783 1101 7 7.640552321306789 1101 8 6.207016123664715 1101 9 6.17732002569781 1101 10 5.742945402310788 1101 11 8.627794729371946 1101 12 6.488713396274733 1101 13 10.817579618623988 1101 14 8.122657470309637 1101 15 7.180580194710323 1101 16 6.804914596355509 1101 17 2.2581930952829445 1101 18 5.080405055453095 1101 19 5.833730355369546 1101 20 3.5937494540918884 1101 21 1.0446323878748132 1101 22 4.785375304065944 1101 23 6.091707088294552 1101 24 7.4022688449475496 1101 25 7.709918621619465 1102 1 5.6571116620717525 1102 2 10.673248380805427 1102 3 6.813423993517315 1102 4 8.203760202234285 1102 5 8.081147454581895 1102 6 7.052881876568705 1102 7 7.062783815288416 1102 8 7.130346941221294 1102 9 6.000403340428573 1102 10 5.557946341297885 1102 11 8.116580920237904 1102 12 5.637544623087951 1102 13 9.980639157492735 1102 14 7.669034574615157 1102 15 5.192110247612501 1102 16 5.844573513721102 1102 17 6.795664881378144 1102 18 6.6161113686354085 1102 19 5.474716610724432 1102 20 5.8992666233824025 1102 21 4.920845541489559 1102 22 5.972368383908194 1102 23 6.37492065648096 1102 24 7.753642246454888 1102 25 7.57253860822561 1103 1 5.510486820555553 1103 2 9.549397843782154 1103 3 6.559500153181546 1103 4 8.561478181219698 1103 5 7.836233509464652 1103 6 6.827751081890845 1103 7 7.031571614585985 1103 8 7.048066523261255 1103 9 5.976441318248124 1103 10 5.036789664575693 1103 11 5.7171398033460825 1103 12 4.716021429803295 1103 13 8.689061751817924 1103 14 6.960790502273678 1103 15 5.782180053351436 1103 16 5.83247773849849 1103 17 3.447211829016549 1103 18 2.113488188466654 1103 19 4.090661174183531 1103 20 4.800817214326603 1103 21 6.741212349516491 1103 22 2.052659556906928 1103 23 4.4859686169583775 1103 24 5.892299178208486 1103 25 1.638282061205107 1104 1 5.8950670587350125 1104 2 10.376282939879312 1104 3 7.109910629540465 1104 4 7.901506627157676 1104 5 8.082254025913187 1104 6 7.496319057365437 1104 7 7.253763130916607 1104 8 7.240408228248874 1104 9 6.162250037738751 1104 10 4.973453236088488 1104 11 8.242634291024423 1104 12 4.81576644807944 1104 13 9.25713302878998 1104 14 7.195528445912398 1104 15 6.247013209818832 1104 16 6.0834652399723765 1104 17 6.548824993737689 1104 18 3.956254371699764 1104 19 4.822403451037676 1104 20 4.032257345207395 1104 21 3.077191302470151 1104 22 5.212483722689264 1104 23 5.692389587362811 1104 24 6.598871221599595 1104 25 5.46247732119803 1105 1 3.678234433675989 1105 2 9.567664560855343 1105 3 6.422263940375675 1105 4 5.499294485113957 1105 5 2.4414532593104035 1105 6 4.895823959734685 1105 7 5.857123441168529 1105 8 3.5196557909519797 1105 9 3.9931521500627234 1105 10 3.8188819156429687 1105 11 7.156484352130441 1105 12 4.204100406360695 1105 13 5.035062992959631 1105 14 3.929843989061581 1105 15 4.585519723164943 1105 16 4.8314202714975245 1105 17 5.440734174917036 1105 18 2.9846270073134633 1105 19 2.641870752373677 1105 20 1.8832202857956766 1105 21 2.8264882360590207 1105 22 4.1337706640552625 1105 23 1.7453235442552923 1105 24 4.7635779821473285 1105 25 5.454311335030212 1106 1 6.607080864085301 1106 2 11.148834781547409 1106 3 7.914786538917795 1106 4 9.194009276524692 1106 5 8.912250588896516 1106 6 7.862798120205557 1106 7 7.780863649333288 1106 8 7.3561068018869555 1106 9 6.353987234851658 1106 10 5.769962158010927 1106 11 8.003720459896506 1106 12 5.567760633792908 1106 13 4.221012131728628 1106 14 5.960132186532206 1106 15 5.934688640878008 1106 16 6.153544577773829 1106 17 6.496465983025658 1106 18 6.428328611259822 1106 19 6.351652104872812 1106 20 5.745862560880191 1106 21 5.663363754781546 1106 22 6.26739185409787 1106 23 6.5488708699428635 1106 24 7.379951160878967 1106 25 6.257459276860516 1107 1 3.902325396300615 1107 2 -13.943792817696446 1107 3 -5.54276310501176 1107 4 1.7434155910987634 1107 5 4.530324112251428 1107 6 4.958715366828784 1107 7 4.737982066078278 1107 8 5.203799120410714 1107 9 3.5483967704287807 1107 10 2.8704345717918898 1107 11 5.68123997072647 1107 12 2.624415085289531 1107 13 4.51975576452724 1107 14 3.8831977365844814 1107 15 3.673498735466371 1107 16 3.4245224602195385 1107 17 4.059474435078474 1107 18 -0.9421712250498544 1107 19 1.95602140431345 1107 20 2.047107311349258 1107 21 -1.2761289108360963 1107 22 1.8696859827197372 1107 23 -1.2159765460382381 1107 24 1.9512391748753686 1107 25 2.8636115687009926 1108 1 5.324243962911918 1108 2 10.61206303815941 1108 3 0.3892666901465276 1108 4 6.03360634050739 1108 5 7.570255663914825 1108 6 7.674554110600489 1108 7 7.806153589557066 1108 8 7.48639512423577 1108 9 5.974110193348906 1108 10 5.039285545670797 1108 11 7.300460867601 1108 12 2.7810510508640434 1108 13 3.4545838887408387 1108 14 5.170907233791187 1108 15 5.29308200588763 1108 16 5.055434936942071 1108 17 0.29955697571967477 1108 18 3.802446017891409 1108 19 5.224671059555284 1108 20 5.242029048729098 1108 21 5.490346503125352 1108 22 2.1237501027056425 1108 23 4.7133304145536385 1108 24 5.617186127414502 1108 25 6.396220154940863 1109 1 3.8935453068502426 1109 2 -13.943792817696446 1109 3 -5.703666514112911 1109 4 2.1276637086209043 1109 5 2.0841241306390508 1109 6 2.7746823123743845 1109 7 3.7637031042217792 1109 8 3.784256683762383 1109 9 3.4368226638058097 1109 10 2.6451627684889694 1109 11 0.6882921645320046 1109 12 1.8244661019267205 1109 13 2.8873198192502443 1109 14 3.5019817739654364 1109 15 3.5268322130163448 1109 16 3.513130967919807 1109 17 3.7962132641475 1109 18 3.994756527820506 1109 19 2.497748687037557 1109 20 1.4403554487011903 1109 21 0.9731639769399223 1109 22 2.890540610541649 1109 23 3.442817694670154 1109 24 4.37873606069725 1109 25 4.737844741773582 1110 1 3.6643547633675446 1110 2 9.569617143989378 1110 3 6.697695808920475 1110 4 8.183300862615706 1110 5 8.718443506930253 1110 6 4.693245215498489 1110 7 7.187638522766136 1110 8 7.8616203494609955 1110 9 6.21957980088748 1110 10 5.756749561410672 1110 11 3.5372942527102467 1110 12 6.295978046825521 1110 13 10.88672637605574 1110 14 8.335658191645596 1110 15 7.6221438263287595 1110 16 7.110415779853952 1110 17 7.719682273431175 1110 18 7.682569244566359 1110 19 6.790152057794906 1110 20 6.993975823089663 1110 21 4.546518192055661 1110 22 6.837429299143459 1110 23 7.298765849989074 1110 24 8.195331514180577 1110 25 8.728238380062535 1111 1 4.785829751326975 1111 2 9.040259715136576 1111 3 7.11346931678818 1111 4 7.875558236117822 1111 5 8.190773555164423 1111 6 7.3299721928478405 1111 7 7.391063076905578 1111 8 7.383039986734289 1111 9 5.93175263879623 1111 10 5.126860550318973 1111 11 7.002621001468448 1111 12 6.225756356544959 1111 13 7.912780734742562 1111 14 6.001113612778052 1111 15 5.526378153817923 1111 16 5.204215424746948 1111 17 6.609820146958455 1111 18 6.429305710604758 1111 19 5.377492924572273 1111 20 5.045363903283462 1111 21 7.661480488893485 1111 22 6.952131016452412 1111 23 6.96048150560717 1111 24 6.860515573182969 1111 25 7.451646688416179 1112 1 -2.6345873376412356 1112 2 7.276642376248683 1112 3 5.501849471603482 1112 4 -0.5656325388639409 1112 5 3.8150526842085313 1112 6 2.8315767908019787 1112 7 5.717184298601019 1112 8 5.848454155831224 1112 9 4.412892400343036 1112 10 2.9662188176371806 1112 11 -0.06756950903864833 1112 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4.135451983263067 2204 7 4.93307462824281 2204 8 5.102750134708426 2204 9 3.5618155457601404 2204 10 3.277186471807017 2204 11 4.628926307831144 2204 12 4.97970096127866 2204 13 3.1543138708237635 2204 14 4.277349810821999 2204 15 4.361583280271255 2204 16 4.217526440210047 2204 17 -1.2046472284845322 2204 18 2.5588772390285115 2204 19 3.392513692103801 2204 20 3.8020588264059327 2204 21 5.161951721789393 2204 22 4.472237245310435 2204 23 3.24811225521783 2204 24 4.975033049967305 2204 25 5.03127453705407 2205 1 6.005384147144884 2205 2 10.21178356468406 2205 3 7.063118611421128 2205 4 7.544967654961489 2205 5 7.92878635955892 2205 6 7.539469635309828 2205 7 7.705682453003455 2205 8 7.14270438569967 2205 9 6.072483168010542 2205 10 5.433514545054436 2205 11 7.610263547506772 2205 12 6.644589472134424 2205 13 10.053114753251084 2205 14 7.3695951438193 2205 15 6.214757664676689 2205 16 5.840064740128575 2205 17 6.543972994362498 2205 18 6.564970397941565 2205 19 5.152017243602501 2205 20 5.42632365261133 2205 21 -0.7640106656648058 2205 22 3.9800661188154756 2205 23 5.271456675772683 2205 24 7.027272502449328 2205 25 7.432486693269258 2206 1 3.898377503310517 2206 2 8.23229615357325 2206 3 5.912899683659038 2206 4 1.3671041571377351 2206 5 5.626339458552835 2206 6 5.858182595433792 2206 7 6.279001321440941 2206 8 6.026510680013974 2206 9 4.784461786833031 2206 10 3.680704443716202 2206 11 3.589840423245615 2206 12 4.4506258156195315 2206 13 7.600058928191782 2206 14 0.027255704597489938 2206 15 2.124628566349852 2206 16 3.1558983289717304 2206 17 4.499193776561295 2206 18 -1.2047954218801493 2206 19 0.9117161212991434 2206 20 2.677701885606755 2206 21 3.4622332183855633 2206 22 -2.33193959997201 2206 23 -2.8508840315885173 2206 24 1.1339959863930353 2206 25 2.701166343019583 2207 1 1.3486492477378533 2207 2 -13.943792817696446 2207 3 -6.290465623428998 2207 4 -4.623674075664038 2207 5 1.0194607006915604 2207 6 1.610397219483751 2207 7 2.589937373340849 2207 8 2.8837682143430854 2207 9 0.28434394526525963 2207 10 0.1909176749319883 2207 11 -1.3770079970571594 2207 12 -2.3458485064370436 2207 13 0.43120525197787885 2207 14 -5.538461791065409 2207 15 -1.4975403564364562 2207 16 -0.431563151012039 2207 17 0.7495698949486531 2207 18 0.7418444985900798 2207 19 0.1859547141467779 2207 20 0.2254011397493727 2207 21 -3.2706598186840274 2207 22 -4.2019178214326445 2207 23 -1.3160100157428154 2207 24 0.44885890918288496 2207 25 1.0423486926564003 2208 1 4.779350411041623 2208 2 6.2798207780101745 2208 3 6.318604793903237 2208 4 7.842027104742108 2208 5 3.267609368245271 2208 6 6.056945446480199 2208 7 6.530008929691476 2208 8 6.332252430558192 2208 9 4.929331067174431 2208 10 4.670143416063549 2208 11 6.738255191441834 2208 12 6.253069939162 2208 13 8.964026426090353 2208 14 2.7201022248466415 2208 15 4.919981714182686 2208 16 5.379048532648692 2208 17 6.110300098090704 2208 18 0.6289653816200769 2208 19 3.2555853369811913 2208 20 4.531205344882139 2208 21 7.166890847111556 2208 22 6.160495255729867 2208 23 1.9167841366120122 2208 24 3.4592109787416767 2208 25 5.01687641048084 2209 1 3.5298622765380436 2209 2 5.035699369803554 2209 3 5.490829190146528 2209 4 0.866853454809192 2209 5 5.5820764690950035 2209 6 5.898899549789679 2209 7 6.463960810840849 2209 8 4.800422432794328 2209 9 4.869547622966024 2209 10 3.924329183058184 2209 11 -0.36543862564224483 2209 12 3.4839719405036824 2209 13 2.270272233335433 2209 14 3.833016608791187 2209 15 4.363253740121863 2209 16 4.225747436942072 2209 17 1.683916139571025 2209 18 3.6083317864724904 2209 19 4.250019454825555 2209 20 4.440549248616485 2209 21 6.6907388876974245 2209 22 5.464939925340779 2209 23 1.3608318222112956 2209 24 -2.2235127746125265 2209 25 0.9229709291525765 2210 1 -2.3516101089817596 2210 2 -13.943792817696446 2210 3 -7.67806004252674 2210 4 -2.0170671052155598 2210 5 -0.7307522747168775 2210 6 -1.2194250170420027 2210 7 -1.1645180722037054 2210 8 -21.055046317205672 2210 9 -13.094911303395362 2210 10 -7.08817855209033 2210 11 -10.271495246929373 2210 12 -4.679309309496318 2210 13 -5.579534387951696 2210 14 -4.15681553848604 2210 15 -5.836050096511801 2210 16 -5.126743280879712 2210 17 -4.736220385800262 2210 18 -4.821423396077016 2210 19 -10.561494313738802 2210 20 -7.352761781705297 2210 21 -5.5049081574758425 2210 22 -10.0203226829513 2210 23 -8.113170343630287 2210 24 -11.006870602582824 2210 25 -8.506919618495939 2211 1 0.07248064180186375 2211 2 4.905453413459332 2211 3 0.7500707102470301 2211 4 1.6751066347715025 2211 5 0.8172700149492762 2211 6 -0.0839953936776574 2211 7 -20.91006262665915 2211 8 -13.544493553386577 2211 9 -9.283647061393271 2211 10 -7.385889881012169 2211 11 -9.966400466094505 2211 12 -5.013982676330489 2211 13 -6.966901918674617 2211 14 -4.872308846610824 2211 15 -5.120422453345473 2211 16 -11.746575113309184 2211 17 -7.80286051715008 2211 18 -5.751146267861682 2211 19 -8.755812439018666 2211 20 -6.89068406639608 2211 21 -4.542606550430715 2211 22 -5.89538611894314 2211 23 -7.888308410200764 2211 24 -22.209840899612526 2211 25 -21.992068133347423 2212 1 -0.8715920068370941 2212 2 -13.943792817696446 2212 3 -7.666474669942659 2212 4 -19.864591857690808 2212 5 -20.349564155904996 2212 6 -21.13625670021032 2212 7 -9.881077119412774 2212 8 -9.370542415310466 2212 9 -4.768304462109228 2212 10 -5.222936877421191 2212 11 -4.908111249666771 2212 12 -2.434493255984613 2212 13 -6.137889521123876 2212 14 -3.421066010355972 2212 15 -3.397432923757737 2212 16 -3.4872629448862433 2212 17 -3.5654796327951956 2212 18 -2.692727081629517 2212 19 -3.3700530960384363 2212 20 -3.3111632740284414 2212 21 -1.2695769654937692 2212 22 -2.745016657365465 2212 23 -2.7373731318020837 2212 24 -1.614523173860018 2212 25 -2.099091544718661 2213 1 -18.949629910961956 2213 2 -13.943792817696446 2213 3 -8.570544184557964 2213 4 -5.702653323411848 2213 5 -20.349564155904996 2213 6 -10.846658591463276 2213 7 -20.91006262665915 2213 8 -10.662611329026003 2213 9 -8.356763233269199 2213 10 -7.161506735972548 2213 11 -6.693609798810094 2213 12 -5.257559900512867 2213 13 -19.350821516664567 2213 14 -12.426019480156803 2213 15 -7.15117075751407 2213 16 -6.261822746273058 2213 17 -4.7932997107540345 2213 18 -4.8472057615476025 2213 19 -5.666051577975864 2213 20 -5.580512641897698 2213 21 -3.6271795335496186 2213 22 -4.7387506959653685 2213 23 -4.451234150010926 2213 24 -3.4970749421657175 2213 25 -4.392777353205581 2214 1 -18.949629910961956 2214 2 -13.943792817696446 2214 3 -12.39529121769905 2214 4 -19.864591857690808 2214 5 -20.349564155904996 2214 6 -21.13625670021032 2214 7 -16.859004096148567 2214 8 -15.150936728164577 2214 9 -14.25372910298354 2214 10 -14.104211251250659 2214 11 -14.559345264807874 2214 12 -12.528126245984486 2214 13 -19.350821516664567 2214 14 -16.92996449721753 2214 15 -23.972683759878137 2214 16 -18.452908774141363 2214 17 -23.649091672928975 2214 18 -17.954704532409828 2214 19 -15.039429778518157 2214 20 -13.889014448067824 2214 21 -11.442189145848653 2214 22 -12.485199444600067 2214 23 -12.440718029905765 2214 24 -11.24097414992386 2214 25 -10.988332143310064 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 103342.0 3 3.0 4 531.0 5 2098.0 6 2654.0 7 2568.0 8 2238.0 9 2085.0 10 2025.0 11 1826.0 12 1717.0 13 1907.0 14 2943.0 15 6176.0 16 8929.0 17 9440.0 18 9653.0 19 11094.0 20 13202.0 21 15330.0 22 16153.0 23 17574.0 24 18670.0 25 19327.0 26 16426.0 27 13004.0 28 13763.0 29 16146.0 30 15518.0 31 17874.0 32 23002.0 33 28918.0 34 35151.0 35 40400.0 36 39766.0 37 41359.0 38 30449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78931686295728 18.345519735112457 11.82201630018556 26.043147101744708 2 13.814710371769332 20.608749644270823 41.15437352857476 24.422166455385096 3 19.374848566783363 23.564413106303483 28.63202694018871 28.42871138672444 4 11.364813684973926 16.11276750048924 39.433131113132035 33.08928770140479 5 12.319165371301056 37.32960776647181 37.488103298652504 12.863123563574637 6 32.420607819702916 37.292594719605134 17.78660344265603 12.500194018035918 7 27.92629399284011 31.486551515460835 22.066232601547178 18.52092189015187 8 24.461421589003397 38.114187223185745 20.243632376338383 17.180758811472472 9 26.15332276774231 15.355841914418713 19.941087149530773 38.549748168308206 10 13.72071035383104 29.864840311705095 35.40295554568579 21.01149378877808 11 33.72497304586943 22.124161085610535 24.6931443441981 19.457721524321933 12 23.40288794553013 24.49841270400231 32.29808415221843 19.800615198249133 13 28.465966853441145 21.326571624465092 25.253958121537384 24.953503400556386 14 19.914618901771302 22.488181759982748 27.865101908102446 29.732097430143504 15 22.244832113025176 32.106879533477525 23.272519934924485 22.37576841857281 16 21.34487837048225 28.32811095810368 28.723120713751193 21.60388995766288 17 20.972409932970198 28.60558217635254 29.18031835742857 21.241689533248692 18 20.832225069105185 27.66564201325877 31.24076212665524 20.261370790980806 19 22.757051957185208 26.963662609737305 27.26835292257064 23.01093251050685 20 22.840641199139483 27.929934929126947 28.55088462472028 20.67853924701329 21 21.568437160439696 26.514948018570863 26.930522171175653 24.986092649813788 22 22.091598860279806 28.757041108446973 28.028455935256346 21.122904096016875 23 21.496589607846392 28.292978409147484 28.461257613223363 21.749174369782764 24 22.433739587389507 27.24928763005528 28.911848085972043 21.405124696583172 25 22.069725489284938 27.763901865862987 28.65210774640992 21.514264898442153 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 567.0 1 567.0 2 1241.5 3 1916.0 4 1916.0 5 1916.0 6 2746.0 7 3576.0 8 3576.0 9 3576.0 10 4111.0 11 4646.0 12 4646.0 13 4646.0 14 5705.0 15 6764.0 16 6764.0 17 6764.0 18 9541.0 19 12318.0 20 12318.0 21 12318.0 22 17604.0 23 22890.0 24 22890.0 25 22890.0 26 30301.0 27 37712.0 28 37712.0 29 37712.0 30 46215.5 31 54719.0 32 54719.0 33 54719.0 34 61088.5 35 67458.0 36 67458.0 37 67458.0 38 70785.0 39 74112.0 40 74112.0 41 74112.0 42 75327.5 43 76543.0 44 76543.0 45 76543.0 46 75073.0 47 73603.0 48 73603.0 49 73603.0 50 67726.0 51 61849.0 52 61849.0 53 61849.0 54 53062.0 55 44275.0 56 44275.0 57 44275.0 58 36772.0 59 29269.0 60 29269.0 61 29269.0 62 23028.5 63 16788.0 64 16788.0 65 16788.0 66 12652.0 67 8516.0 68 8516.0 69 8516.0 70 6115.0 71 3714.0 72 3714.0 73 3714.0 74 2525.5 75 1337.0 76 1337.0 77 1337.0 78 907.0 79 477.0 80 477.0 81 477.0 82 315.5 83 154.0 84 154.0 85 154.0 86 98.0 87 42.0 88 42.0 89 42.0 90 27.5 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.989236831156001 2 35.926572412272634 3 5.586802395646329 4 13.600580843117655 5 13.088198971920942 6 14.561690545220063 7 9.058931374645468 8 7.480178562844275 9 0.11205763342898016 10 1.4675240070218363 11 5.598737528200895 12 5.8538509865547415 13 15.863283056587447 14 1.6057726257788916 15 2.8975849590807297 16 2.976986743714578 17 2.859956138387862 18 1.5315095787727038 19 1.47316004183927 20 0.29224498185694087 21 4.347703564460491 22 1.6215203701217218 23 0.11454411937784806 24 8.899464742458074 25 0.11570447948731975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 603261.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.41737798953531 #Duplication Level Percentage of deduplicated Percentage of total 1 97.27912740684606 90.87561015457503 2 1.9781052417714953 3.6957881014729783 3 0.33761369234769056 0.9461695773746068 4 0.12985061057557584 0.4852121428124208 5 0.07796497078282424 0.36416415727810847 6 0.043384620992336975 0.2431726522904323 7 0.028649850499339193 0.18734757394083124 8 0.019381174097354962 0.14484307732268795 9 0.014756260296975472 0.12406420312870775 >10 0.07954835184966468 1.4033486025269966 >50 0.005990451308823084 0.41631716575011024 >100 0.00508278457100598 0.8221176197364498 >500 5.445840609731163E-4 0.2918449717906467 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 695 0.11520718229754616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.972971897735806E-4 0.0 10 0.0 0.0 0.0 4.972971897735806E-4 0.0 11 0.0 0.0 0.0 4.972971897735806E-4 0.0 12 0.0 0.0 0.0 4.972971897735806E-4 0.0 13 0.0 0.0 0.0 4.972971897735806E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 510 0.0 15.475476 2 TAGGACC 225 0.0 15.189243 4 CTATAGT 65 1.8045932E-5 14.787606 4 GTATTAA 55 0.0046867346 14.503283 1 CCCAGGA 125 2.4045221E-8 14.152056 2 CTGTAGG 470 0.0 14.143271 1 GAACCAC 45 0.0064756563 13.814389 6 CTATACC 70 3.6295627E-5 13.731348 4 TCCTAGA 90 3.9468032E-5 13.607746 2 TGTAGAA 170 4.1836756E-11 13.607746 2 GTCTATG 60 0.00834067 13.294675 1 GATCTAC 60 0.00834067 13.294675 1 GTAGGAC 525 0.0 13.272365 3 CTTAGAG 75 5.7908863E-5 13.064983 3 GTCCTAC 400 0.0 12.962309 1 CCAGGAC 145 1.2369128E-10 12.76464 3 TCTATAC 60 0.0021152752 12.702066 3 AGGACGT 480 0.0 12.526353 5 CTTACAC 70 4.742945E-4 12.4428425 3 GGCGAGG 195 0.0 12.26567 19 >>END_MODULE