FastQCFastQC Report
Thu 26 May 2016
SRR1926449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603261
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14580.24168643422996017No Hit
CTGTAGGACGTGGAATATGGCAAGA13520.22411526685796032No Hit
GTCCTACAGTGTGCATTTCTCATTT10830.17952428550826258No Hit
CTGTAGGACCTGGAATATGGCGAGA9740.16145582094648916No Hit
GTCCTAAAGTGTGTATTTCTCATTT9700.16079275802679108No Hit
CTTTAGGACGTGAAATATGGCGAGG9620.15946663218739485No Hit
CCCATGTACTCTGCGTTGATACCAC8600.14255852773509312No Hit
GTACATGGGAAGCAGTGGTATCAAC8440.13990627605630065No Hit
GTATCAACGCAGAGTACTTTTTTTT6580.10907385029033868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT350.002211001216.2380419
GAGTGAT456.7636726E-414.7749347
TGACGAA456.905628E-414.73451818
GACGAAA456.905628E-414.73451819
TCAGCGC400.00512838514.3102983
CTAGTAC400.00512838514.3102983
TAGGACT801.2218152E-714.3079094
GTCTAAG602.4727267E-514.3043271
GCAATTT400.005345270514.22125213
TCCAACG602.6349597E-514.20828518
AATCCCG1400.013.53170119
TAGGAGT603.9480854E-412.7181424
GTACTAA853.7681311E-612.3409881
GGTCTAC853.7681311E-612.3409881
GTCCTAC7350.012.3257241
TCATAAC550.002969250312.1420713
CTAGGAC1103.5792254E-812.1420713
GCTTAGG550.002978550512.1370051
ACTGGTC550.0030652912.0905918
ACTGTTC2750.011.7451468
Produced by FastQC (version 0.11.5)