FastQCFastQC Report
Thu 26 May 2016
SRR1926448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784532
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10920.1391912630714872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTCG304.97739E-420.277747
GGTCTAA405.690854E-419.877231
GTGCTAC500.002541152315.9017831
ATAGGAC855.189031E-815.3432113
TAGGAGT702.1674896E-615.2465564
TTTAGAC505.411486E-415.215353
TAGGACT1155.820766E-1114.848824
GTCCTAA2700.014.7238721
TAGGACA2000.014.4079964
GGGTAGG658.791926E-414.270831
GTCCTAG751.613177E-414.1349191
GTCTTAG751.613177E-414.1349191
GTCTATG852.962802E-514.0309861
GTCCTAC6900.013.8276381
TGTAGGA6350.013.6856192
CCTATAC807.167271E-613.58513453
TAGGACC2600.013.54597954
CTGTAGG6850.013.1547591
TTAGGAC3650.013.1012813
TCCTACA7500.012.8545042
Produced by FastQC (version 0.11.5)