FastQCFastQC Report
Thu 26 May 2016
SRR1926448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784532
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA21670.27621562919039633No Hit
GTCCTACAGTGGACATTTCTAAATT20830.2655086089541281No Hit
GTCCTAAAGTGTGTATTTCTCATTT15560.19833480342420703No Hit
CTTTAGGACGTGAAATATGGCGAGG15180.1934911514125619No Hit
GTCCTACAGTGTGCATTTCTCATTT14150.18036230517047108No Hit
CTGTAGGACCTGGAATATGGCGAGA12590.16047783901740145No Hit
CCCATGTACTCTGCGTTGATACCAC9200.11726736449246175No Hit
CTGAAGGACCTGGAATATGGCGAGA8990.11459060943339468No Hit
GTACATGGGAAGCAGTGGTATCAAC8540.10885470573539385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTT350.002193383616.25837910
TACACCG400.00516146714.2972115
CGTGCGC400.005335141414.2260810
GTTCTAG1403.6379788E-1212.9339141
TCTATAC1051.8928404E-812.7110773
TAGGACC4050.012.7086324
GGCGAGG4400.012.70833719
TAGACTG1251.3333192E-912.2002885
TGCGCCA701.1067907E-412.19378412
TCTAGGA1501.2732926E-1112.0778462
ATACGGC550.003119794712.0629215
TAAGACA1353.4924597E-1012.0025974
GTCCTAC9850.011.9974751
TGTAGGA11800.011.7977182
GTACTAG657.804588E-411.7295421
TCCTACA10600.011.6940412
GTAGGAC11750.011.68337253
TCTGGAC907.1625454E-611.651823
CTGTAGG12000.011.595141
TTAGGAC6600.011.2666363
Produced by FastQC (version 0.11.5)