FastQCFastQC Report
Thu 26 May 2016
SRR1926447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760100
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9560.12577292461518222No Hit
GTCCTAAAGTGTGTATTTCTCATTT7740.10182870674911196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAT300.00239282422.2838361
GGACGTA350.001163365617.8725976
CTCTAGG808.307725E-716.7128771
TAGGACC2250.016.693384
GTCCTAC5450.014.2289441
CTAGCAC550.001048986713.9421153
TGTAGGA6500.013.6987012
TATTAAG600.00684564113.69872
CTGTAGG6550.013.6764921
AGGACCT4300.013.5837945
GTCCTAA3550.013.5586151
GATATAC1305.081165E-813.3703011
TGTAGAA1756.184564E-1113.3073082
GTAGGAC6950.012.76713853
GTATAGC1051.3949868E-512.7336211
ACTGTTC1509.094947E-1212.3643678
GGACCTG4050.012.0989386
TCCTACA6800.012.0870892
TTCTGAC800.003267733611.9863642
AAGACTA706.809594E-411.9204735
Produced by FastQC (version 0.11.5)