##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926447_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 760100 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.797797658202867 27.0 10.0 31.0 2.0 31.0 2 16.71714774371793 22.0 2.0 31.0 2.0 31.0 3 20.43413103539008 25.0 15.0 31.0 2.0 31.0 4 22.778126562294435 28.0 7.0 35.0 2.0 37.0 5 23.386480726220235 30.0 7.0 35.0 2.0 37.0 6 24.153708722536507 31.0 8.0 35.0 2.0 37.0 7 23.91260097355611 31.0 10.0 35.0 2.0 37.0 8 24.050542033942904 31.0 12.0 35.0 2.0 37.0 9 26.931833969214576 32.0 17.0 37.0 2.0 39.0 10 27.67555847914748 32.0 25.0 38.0 2.0 39.0 11 24.681994474411262 30.0 10.0 37.0 2.0 39.0 12 25.864311274832257 31.0 17.0 37.0 2.0 39.0 13 22.40557295092751 29.0 2.0 37.0 2.0 39.0 14 25.90138929088278 31.0 17.0 38.0 2.0 40.0 15 27.03053940271017 31.0 18.0 38.0 2.0 40.0 16 27.41703854755953 32.0 22.0 39.0 2.0 40.0 17 26.723653466649125 31.0 17.0 39.0 2.0 40.0 18 26.82315879489541 31.0 17.0 38.0 2.0 40.0 19 27.220756479410603 31.0 22.0 39.0 2.0 40.0 20 27.486604394158665 32.0 24.0 39.0 2.0 40.0 21 25.28523746875411 31.0 10.0 38.0 2.0 40.0 22 26.25115379555322 31.0 16.0 38.0 2.0 40.0 23 26.26747927904223 31.0 10.0 39.0 2.0 40.0 24 25.260057887120116 31.0 2.0 39.0 2.0 40.0 25 25.066324167872647 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.435963343324438 1101 2 11.34330112148594 1101 3 7.773172111855555 1101 4 9.286640603045527 1101 5 9.22131113132426 1101 6 8.62953637771249 1101 7 8.398896474551517 1101 8 6.9362667936155304 1101 9 6.719239900425773 1101 10 6.185228382492472 1101 11 9.060008651657256 1101 12 6.553356710403804 1101 13 11.19603868926795 1101 14 8.534684390747302 1101 15 7.64074909064766 1101 16 7.255364154808994 1101 17 3.1047068824589594 1101 18 5.758636020064188 1101 19 6.174903981437552 1101 20 4.353273107265451 1101 21 1.5815803728588484 1101 22 5.158927431949476 1101 23 6.335802223640233 1101 24 7.949063059988596 1101 25 8.304308015674842 1102 1 6.063606112161246 1102 2 11.285353539724941 1102 3 7.484298035950808 1102 4 8.708241530810184 1102 5 8.504429028561216 1102 6 7.543940921856443 1102 7 7.469619965062986 1102 8 7.615118783476952 1102 9 6.405100017510232 1102 10 5.834168200199951 1102 11 8.737266408604036 1102 12 5.901816111707852 1102 13 10.603921556079307 1102 14 8.080476526677124 1102 15 6.005701956931084 1102 16 6.431679469481452 1102 17 7.004681530210647 1102 18 7.045447107524808 1102 19 5.991680904277956 1102 20 6.3110537818905215 1102 21 4.985207890896945 1102 22 6.28560455279268 1102 23 6.685529237360033 1102 24 8.087180020294632 1102 25 7.813398566131909 1103 1 5.048731522039834 1103 2 9.392080885417094 1103 3 6.571700042246494 1103 4 7.982923243203768 1103 5 7.404879887554632 1103 6 6.876911111257769 1103 7 6.979576608420466 1103 8 6.470899361060656 1103 9 5.418780227835562 1103 10 4.6273276180009155 1103 11 5.657420658312297 1103 12 4.3955421890795705 1103 13 8.62452058256735 1103 14 6.665971281960452 1103 15 5.71829112546267 1103 16 5.49793266487972 1103 17 3.205964042474953 1103 18 2.0788163974740677 1103 19 4.233414907608861 1103 20 4.605513421930805 1103 21 6.893021209871481 1103 22 2.3402598015939766 1103 23 4.501827303991277 1103 24 6.102411791393504 1103 25 1.7838724003891997 1104 1 5.931780468538577 1104 2 10.80787926613872 1104 3 7.324772932604169 1104 4 8.390625232056603 1104 5 8.124648268533107 1104 6 7.926456349169545 1104 7 7.637943117839619 1104 8 7.511353352607532 1104 9 6.3250384505106005 1104 10 5.406087503047562 1104 11 8.450541721773511 1104 12 5.407260125284456 1104 13 9.85137480470586 1104 14 7.415053338906311 1104 15 6.561068727252604 1104 16 6.220500108651606 1104 17 6.6812533522126 1104 18 4.19295697275453 1104 19 5.138802703946219 1104 20 4.427528644403218 1104 21 3.4448846356983154 1104 22 5.68434703829001 1104 23 6.121791744934992 1104 24 7.204838425868665 1104 25 6.198322048365959 1105 1 3.804873344701832 1105 2 9.764559014479321 1105 3 6.466470907829006 1105 4 5.650736547239347 1105 5 2.6019944565184367 1105 6 4.876531433884082 1105 7 5.948374031528484 1105 8 2.8811726468670393 1105 9 3.77722316449087 1105 10 3.439713173646176 1105 11 6.630234237306837 1105 12 4.279521207955796 1105 13 5.414650842912533 1105 14 3.988358908573204 1105 15 4.3484695679781105 1105 16 4.608374904192296 1105 17 5.235495219896023 1105 18 2.571607792545631 1105 19 2.455173404718284 1105 20 1.5607875443144543 1105 21 2.5035389166435458 1105 22 3.8936357711168235 1105 23 1.561127761593191 1105 24 4.6468685602396 1105 25 5.36151379072211 1106 1 6.328355258952158 1106 2 11.031652863458646 1106 3 7.744600986753952 1106 4 8.988328965408943 1106 5 8.602708175432323 1106 6 7.464076083464956 1106 7 7.450800675835705 1106 8 7.200986158296658 1106 9 6.130074555849365 1106 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1.3082593861682312 2207 10 1.0095626412472214 2207 11 -0.7412921275994648 2207 12 -1.6382418469766478 2207 13 1.0420295388394045 2207 14 -4.860588176129529 2207 15 -1.1573291463813007 2207 16 0.2046060876424285 2207 17 1.2142473718079643 2207 18 1.347138307671326 2207 19 0.6521221819685898 2207 20 0.5903851005539842 2207 21 -3.2367118354388076 2207 22 -3.8510532812304277 2207 23 -0.8584912992747604 2207 24 0.9796019666075182 2207 25 1.5332105696649663 2208 1 4.834415794104434 2208 2 6.67979774194481 2208 3 6.282243738521963 2208 4 7.927936338882777 2208 5 3.74202815743579 2208 6 6.077755258006878 2208 7 6.828711950366884 2208 8 6.7174042735319155 2208 9 5.037732230275882 2208 10 4.6202253366234665 2208 11 6.768772405869903 2208 12 6.098884507526847 2208 13 8.743604067728622 2208 14 3.2793899378914837 2208 15 4.724782219791969 2208 16 4.859285381257372 2208 17 5.78960836704406 2208 18 0.4322314327861747 2208 19 3.256497183682715 2208 20 4.33581551109436 2208 21 6.888525331231971 2208 22 5.972983996241549 2208 23 1.9605220764043771 2208 24 3.2758936918973447 2208 25 4.583214820700984 2209 1 2.9267328835005166 2209 2 4.846414927993035 2209 3 5.321845276599497 2209 4 0.6850431301526356 2209 5 5.351123574760823 2209 6 5.657102927524729 2209 7 6.076211804673953 2209 8 4.585429568024065 2209 9 4.508317651966205 2209 10 3.5056782547154484 2209 11 -0.5904596329404939 2209 12 3.0617740436955287 2209 13 2.160724031838683 2209 14 3.863459707762626 2209 15 4.22154508777658 2209 16 3.839450800462668 2209 17 1.5172983808656504 2209 18 3.2968425646058392 2209 19 3.8791981217252847 2209 20 4.023672527501034 2209 21 6.234245312715224 2209 22 4.8617669599546645 2209 23 0.5771638378264257 2209 24 -2.9340322301190014 2209 25 0.047439783782945 2210 1 -2.3479278540223554 2210 2 -14.214260955263494 2210 3 -7.938782664735244 2210 4 -2.2108067137915164 2210 5 -0.6308015869208603 2210 6 -1.3983861779181481 2210 7 -1.3778458919613072 2210 8 -21.5159529204552 2210 9 -13.693779562510297 2210 10 -7.46783016447306 2210 11 -10.857053408317915 2210 12 -5.308195723541548 2210 13 -5.459968533249814 2210 14 -4.457773182352533 2210 15 -6.027441038418299 2210 16 -5.475111802881525 2210 17 -4.934094380882566 2210 18 -5.0779593080643295 2210 19 -10.37654267880697 2210 20 -7.189107663679611 2210 21 -5.3627404612532885 2210 22 -10.181067679551438 2210 23 -7.9893494636108535 2210 24 -10.770407184867699 2210 25 -8.297523134608163 2211 1 -0.00708888109241812 2211 2 5.013082794736505 2211 3 0.7831620008698508 2211 4 1.7533154995842786 2211 5 1.13368885893901 2211 6 0.10507719787803182 2211 7 -21.37386500024156 2211 8 -13.639390420455198 2211 9 -9.405309220153608 2211 10 -7.313207353963506 2211 11 -9.8635323095918 2211 12 -5.141232148700784 2211 13 -7.017995006179751 2211 14 -4.964296868658266 2211 15 -5.4840592709023745 2211 16 -12.132322193008914 2211 17 -8.052078059226517 2211 18 -5.957582118573882 2211 19 -8.91211692243754 2211 20 -6.71911861113184 2211 21 -4.424822467622718 2211 22 -5.687173372226596 2211 23 -7.83516455119047 2211 24 -22.83648998741547 2211 25 -22.6422683575381 2212 1 -1.2148162944587533 2212 2 -14.214260955263494 2212 3 -8.077806439724208 2212 4 -20.33262200041572 2212 5 -20.81631114106099 2212 6 -21.58867280212197 2212 7 -10.249277541495687 2212 8 -9.911992524415595 2212 9 -5.3726385312097165 2212 10 -5.80708885148826 2212 11 -5.691868736984539 2212 12 -3.041673052166132 2212 13 -6.487056990503184 2212 14 -4.198854933839783 2212 15 -3.989736869912278 2212 16 -4.1958870651211235 2212 17 -4.335468116982291 2212 18 -3.4606478157686027 2212 19 -3.9128620750778076 2212 20 -3.9505826169074147 2212 21 -1.9184177646524212 2212 22 -3.120978549619334 2212 23 -3.5491574348538357 2212 24 -2.541935531969923 2212 25 -2.9170208327856244 2213 1 -19.378182631092418 2213 2 -14.214260955263494 2213 3 -8.803927962076965 2213 4 -5.710128539212583 2213 5 -20.81631114106099 2213 6 -11.096083438564865 2213 7 -21.37386500024156 2213 8 -10.892587619670543 2213 9 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21 -11.98092135543703 2214 22 -13.039344854334914 2214 23 -13.04887495979782 2214 24 -11.866470645055701 2214 25 -11.597780930071949 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 123354.0 3 5.0 4 610.0 5 2356.0 6 3273.0 7 3099.0 8 2610.0 9 2510.0 10 2211.0 11 2219.0 12 2078.0 13 2195.0 14 3458.0 15 7797.0 16 11602.0 17 12447.0 18 12626.0 19 14447.0 20 16975.0 21 19668.0 22 20593.0 23 22398.0 24 23624.0 25 24381.0 26 20340.0 27 15858.0 28 16715.0 29 19470.0 30 19137.0 31 21786.0 32 28744.0 33 36204.0 34 44411.0 35 51855.0 36 51914.0 37 54547.0 38 42583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67061544665343 18.182039518233626 11.774364124687104 26.37298091042584 2 13.993952641467958 20.500669854871692 40.617261931919515 24.888115571740833 3 18.958803864129344 23.9380911916635 28.75162123482189 28.35148370938527 4 11.409998272416919 16.297070905819204 39.06692167627539 33.22600914548848 5 12.367966725635693 37.220823272567834 37.2404626987921 13.170747303004376 6 32.48435072348375 36.9274905236873 18.01916794334195 12.568990809487001 7 27.914986632296717 31.33441900565188 22.400990458275636 18.349603903775762 8 24.60130047912389 37.724446725986766 20.1303331051791 17.543919689710243 9 26.557789441104223 15.052715455078266 19.71630458403851 38.673190519779 10 14.16448763463345 29.163131446071127 35.2780052938119 21.39437562548352 11 33.533485683935396 21.668728291786348 25.24155956698884 19.556226457289416 12 23.968185546060422 24.08756672330158 32.016408264032535 19.92783946660546 13 28.515400698179665 21.253262393161048 25.37500806257502 24.856328846084264 14 20.236421169528832 22.20445681333164 27.65030539406873 29.9088166230708 15 22.156854767620054 32.052972049310384 23.111599332763742 22.678573850305817 16 21.57233071599554 27.6929092911192 28.592027959304765 22.142732033580494 17 21.290988274343484 28.394040633033597 28.923647644564003 21.391323448058923 18 21.282140073294944 27.07901868740282 30.966645586459823 20.67219565284242 19 23.146581789319246 26.642828318461092 26.98436877817776 23.22622111404191 20 23.456987211540618 27.41728005446277 28.133291290047218 20.992441443949396 21 21.954628534566176 26.19941610910324 26.50362933128227 25.342326025048312 22 22.640421092640647 28.58890335774004 27.405416763138067 21.365258786481245 23 21.761944650787882 28.050502336510146 28.318529289353204 21.869023723348768 24 22.97277510137393 26.823172261019206 28.39281908685901 21.811233550747854 25 22.327152127728233 27.383807691142554 28.297751558472516 21.991288622656693 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 677.0 1 677.0 2 1523.5 3 2370.0 4 2370.0 5 2370.0 6 3499.5 7 4629.0 8 4629.0 9 4629.0 10 5302.0 11 5975.0 12 5975.0 13 5975.0 14 7090.0 15 8205.0 16 8205.0 17 8205.0 18 11500.0 19 14795.0 20 14795.0 21 14795.0 22 21639.5 23 28484.0 24 28484.0 25 28484.0 26 37434.0 27 46384.0 28 46384.0 29 46384.0 30 57094.0 31 67804.0 32 67804.0 33 67804.0 34 75550.0 35 83296.0 36 83296.0 37 83296.0 38 87552.5 39 91809.0 40 91809.0 41 91809.0 42 93149.5 43 94490.0 44 94490.0 45 94490.0 46 93520.5 47 92551.0 48 92551.0 49 92551.0 50 85475.5 51 78400.0 52 78400.0 53 78400.0 54 68016.0 55 57632.0 56 57632.0 57 57632.0 58 48309.0 59 38986.0 60 38986.0 61 38986.0 62 31098.5 63 23211.0 64 23211.0 65 23211.0 66 17656.0 67 12101.0 68 12101.0 69 12101.0 70 8731.0 71 5361.0 72 5361.0 73 5361.0 74 3650.5 75 1940.0 76 1940.0 77 1940.0 78 1337.5 79 735.0 80 735.0 81 735.0 82 461.0 83 187.0 84 187.0 85 187.0 86 125.0 87 63.0 88 63.0 89 63.0 90 37.5 91 12.0 92 12.0 93 12.0 94 7.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.542297066175504 2 36.561768188396265 3 5.867122747006973 4 13.946454413892909 5 13.584791474805947 6 15.06946454413893 7 9.357452966714906 8 7.738455466386002 9 0.1098539665833443 10 1.5365083541639257 11 5.873569267201684 12 5.996842520720957 13 16.372450993290357 14 1.6714905933429811 15 2.990396000526246 16 3.104986186028154 17 2.9696092619392185 18 1.6001841862912776 19 1.5426917510853835 20 0.28298908038416 21 4.554926983291672 22 1.6972766741218261 23 0.11156426785949218 24 9.252335219050124 25 0.11353769240889355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 760100.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.36486182923682 #Duplication Level Percentage of deduplicated Percentage of total 1 96.64938214890518 89.27006828064734 2 2.4867432799897125 4.59375398922066 3 0.41842636997824983 1.1594368154625052 4 0.15392927142471202 0.5687062354647447 5 0.07068192839879156 0.32642632751891965 6 0.048631937311056944 0.2695129302134329 7 0.030957178505939337 0.2001548860726875 8 0.022547672911325155 0.16660901544202994 9 0.014186439164379392 0.11792956439400988 >10 0.09205184020147131 1.5403493286264927 >50 0.006304521492613994 0.4292810966679595 >100 0.005424386527613803 0.8618944686051544 >500 7.330251889878083E-4 0.4958770616640836 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 956 0.12577292461518222 No Hit GTCCTAAAGTGTGTATTTCTCATTT 774 0.10182870674911196 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.3156163662675965E-4 0.0 0.0 0.0 6 0.0 1.3156163662675965E-4 0.0 0.0 0.0 7 0.0 1.3156163662675965E-4 0.0 0.0 0.0 8 0.0 1.3156163662675965E-4 0.0 0.0 0.0 9 0.0 1.3156163662675965E-4 0.0 1.3156163662675965E-4 0.0 10 0.0 1.3156163662675965E-4 0.0 1.3156163662675965E-4 0.0 11 0.0 1.3156163662675965E-4 0.0 1.3156163662675965E-4 0.0 12 0.0 1.3156163662675965E-4 0.0 1.3156163662675965E-4 0.0 13 0.0 1.3156163662675965E-4 0.0 1.3156163662675965E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGTAT 30 0.002392824 22.283836 1 GGACGTA 35 0.0011633656 17.872597 6 CTCTAGG 80 8.307725E-7 16.712877 1 TAGGACC 225 0.0 16.69338 4 GTCCTAC 545 0.0 14.228944 1 CTAGCAC 55 0.0010489867 13.942115 3 TGTAGGA 650 0.0 13.698701 2 TATTAAG 60 0.006845641 13.6987 2 CTGTAGG 655 0.0 13.676492 1 AGGACCT 430 0.0 13.583794 5 GTCCTAA 355 0.0 13.558615 1 GATATAC 130 5.081165E-8 13.370301 1 TGTAGAA 175 6.184564E-11 13.307308 2 GTAGGAC 695 0.0 12.7671385 3 GTATAGC 105 1.3949868E-5 12.733621 1 ACTGTTC 150 9.094947E-12 12.364367 8 GGACCTG 405 0.0 12.098938 6 TCCTACA 680 0.0 12.087089 2 TTCTGAC 80 0.0032677336 11.986364 2 AAGACTA 70 6.809594E-4 11.920473 5 >>END_MODULE