FastQCFastQC Report
Thu 26 May 2016
SRR1926447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760100
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18680.245757137218787No Hit
GTCCTACAGTGGACATTTCTAAATT18390.24194184975661098No Hit
CTTTAGGACGTGAAATATGGCGAGG15810.207998947506907No Hit
GTCCTAAAGTGTGTATTTCTCATTT15760.20734113932377318No Hit
GTCCTACAGTGTGCATTTCTCATTT14410.18958031837916064No Hit
CTGTAGGACCTGGAATATGGCGAGA12190.16037363504802No Hit
CTGAAGGACCTGGAATATGGCGAGA8990.11827391132745692No Hit
CCCATGTACTCTGCGTTGATACCAC8740.11498487041178793No Hit
GTATCAACGCAGAGTACTTTTTTTT8390.11038021312985134No Hit
GTACATGGGAAGCAGTGGTATCAAC7620.10024996710959085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG350.002109300616.3536835
TAGGGTG706.945389E-613.6280695
ACTGATC500.00149831113.3000018
TAGGATC603.9465606E-412.7195314
TAGGACA3450.012.443024
GAACAGT1251.3169483E-912.2099416
GTATTGG550.002977670912.1381551
GGCATGA550.002977670912.1381551
TGTAGGA12150.012.0970162
CTGTAGG12050.011.8719361
GGCGAGG4650.011.815165519
CTAGGAC1052.5609916E-711.8141243
ATAGGAC1302.4283509E-911.7442183
GTAGGCA657.739805E-411.7411064
TGGCGAG11750.011.69024118
GTCCTAC9650.011.6619731
TAGGACC4350.011.6230214
GTAGGAC12100.011.5137013
GGATTAT751.9974392E-411.4445461
CTAAATT5550.011.43527419