##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926447_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 760100 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.420552558873833 31.0 28.0 31.0 22.0 34.0 2 28.867154321799763 31.0 28.0 33.0 23.0 34.0 3 29.235830811735298 31.0 29.0 33.0 25.0 34.0 4 33.00446520194711 35.0 33.0 37.0 27.0 37.0 5 32.67348506775424 35.0 33.0 37.0 26.0 37.0 6 32.753398237074066 35.0 33.0 37.0 26.0 37.0 7 32.576984607288516 35.0 33.0 37.0 25.0 37.0 8 32.542902249703985 35.0 33.0 37.0 25.0 37.0 9 33.798772529930275 37.0 34.0 39.0 23.0 39.0 10 33.69919615840021 37.0 33.0 39.0 23.0 39.0 11 33.943695566372845 37.0 34.0 39.0 25.0 39.0 12 33.728936981976055 37.0 34.0 39.0 23.0 39.0 13 33.870568346270225 37.0 34.0 39.0 25.0 39.0 14 34.52726088672543 38.0 34.0 40.0 22.0 41.0 15 34.66414945401921 39.0 34.0 40.0 24.0 41.0 16 34.643004867780554 39.0 34.0 40.0 24.0 41.0 17 34.54706222865413 39.0 34.0 40.0 22.0 41.0 18 34.52112748322589 39.0 34.0 40.0 22.0 41.0 19 34.55255098013419 39.0 34.0 40.0 22.0 41.0 20 34.537996316274175 39.0 34.0 40.0 22.0 41.0 21 34.453852124720434 39.0 34.0 40.0 19.0 41.0 22 34.3830732798316 39.0 34.0 40.0 19.0 41.0 23 34.03748191027496 39.0 34.0 40.0 17.0 41.0 24 34.12398368635706 39.0 34.0 41.0 18.0 41.0 25 33.91104328377845 39.0 34.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8450396407249876 1101 2 0.9518045458693294 1101 3 1.0436095853016454 1101 4 0.7185085364542374 1101 5 0.9795750777454586 1101 6 0.4985407878956991 1101 7 0.9218453521322232 1101 8 0.9922937629939099 1101 9 1.2700738540902634 1101 10 1.2266250314890925 1101 11 1.023201112909888 1101 12 1.091686233954043 1101 13 1.0003001711267956 1101 14 1.4301480996668232 1101 15 1.2028904405246195 1101 16 1.0916543653399557 1101 17 1.1615102417802277 1101 18 1.1998626609857652 1101 19 1.2083460457685504 1101 20 1.119245914922729 1101 21 1.1176236451215544 1101 22 1.255530758409897 1101 23 1.5879849150018757 1101 24 1.4052370999485717 1101 25 1.4956455463656937 1102 1 0.659081769548056 1102 2 0.597246101395104 1102 3 0.4403780728630906 1102 4 0.18918317892661918 1102 5 0.4655312665077389 1102 6 -0.011872488560413785 1102 7 0.33301704950876143 1102 8 0.5049591545063024 1102 9 0.6393133968679763 1102 10 0.7311933674011613 1102 11 0.37454150426273713 1102 12 0.5651739897463415 1102 13 0.4004294269831945 1102 14 0.7929051247632231 1102 15 0.3730916283093606 1102 16 0.6267596873976302 1102 17 0.6876419121917223 1102 18 0.4703073347800668 1102 19 0.5979805150798541 1102 20 0.34423928678915416 1102 21 0.44234823674440094 1102 22 0.3491925222882202 1102 23 0.640914259968234 1102 24 0.6555745981283749 1102 25 0.6551459968467199 1103 1 0.4892115055501698 1103 2 0.3541865332680487 1103 3 0.25827301872244135 1103 4 0.23689456715610646 1103 5 0.4404767203810067 1103 6 0.03527182269150586 1103 7 0.3674763675495001 1103 8 0.41073373932068336 1103 9 0.7247464355717312 1103 10 0.8382671607525793 1103 11 0.5494188611950648 1103 12 0.7191052762358936 1103 13 0.5736478899600996 1103 14 0.9028339807525043 1103 15 0.794611096461523 1103 16 0.8416818618963404 1103 17 0.7649068957548337 1103 18 0.7114539870879355 1103 19 0.9355505276199452 1103 20 0.8804937522967435 1103 21 0.5965979450068559 1103 22 0.7048070315877482 1103 23 1.1539984729864372 1103 24 0.9129893830356579 1103 25 0.9961142971840431 1104 1 0.36447855520442474 1104 2 0.4038898748490283 1104 3 0.436095126409878 1104 4 0.06887545773066961 1104 5 0.018472342564606947 1104 6 -0.15496113764274355 1104 7 -0.10503499793538396 1104 8 0.06849066491778188 1104 9 0.4885817908626393 1104 10 0.29283689902537446 1104 11 -0.05142759274511377 1104 12 -0.040715844017071845 1104 13 -0.16863799229547283 1104 14 0.2663360055639359 1104 15 0.0842667215560553 1104 16 0.08065755559758969 1104 17 0.09789833703815987 1104 18 0.14991027884342856 1104 19 0.16408281758322119 1104 20 0.10883940002236159 1104 21 0.28076103721782175 1104 22 0.2645145683382921 1104 23 0.33902542766433896 1104 24 0.18904980489277534 1104 25 0.4196152375298752 1105 1 0.36540860081350246 1105 2 0.3576282004319822 1105 3 0.5388171566558988 1105 4 0.2555087037908166 1105 5 0.3575297921406104 1105 6 -0.18697458031876835 1105 7 0.028006184216224028 1105 8 0.21597211703941355 1105 9 0.5983483862593033 1105 10 0.5836592580810347 1105 11 0.27470970342361056 1105 12 0.19644690279950083 1105 13 0.3217065041903382 1105 14 0.5711860873476624 1105 15 0.3555771033436841 1105 16 0.4825918941542895 1105 17 0.4171066259440437 1105 18 0.537049858257383 1105 19 0.4860382580150784 1105 20 0.5325097864992969 1105 21 0.4162283767626249 1105 22 0.5575436922755159 1105 23 0.6756254578098719 1105 24 0.3940517308575693 1105 25 0.5653577388278066 1106 1 0.503522764368654 1106 2 0.5756125683018176 1106 3 0.29023504076592843 1106 4 0.39314855335909726 1106 5 0.5982171652728638 1106 6 -0.604168574739397 1106 7 -0.4853780123610747 1106 8 -0.3004198146773547 1106 9 -0.2974958589183174 1106 10 -0.28197051175304466 1106 11 -0.5026324747790767 1106 12 -0.4145110990572931 1106 13 -0.7570003944930477 1106 14 -0.29323204628300203 1106 15 -0.4168803151743461 1106 16 -0.1904439176756867 1106 17 -0.16827888342646702 1106 18 -0.4432009212135881 1106 19 -0.44277418115021305 1106 20 -0.33136884307047154 1106 21 -0.35349105864637664 1106 22 -0.2870757662871952 1106 23 -0.14929311079593788 1106 24 -0.27143664395388356 1106 25 -0.3997813071042202 1107 1 0.40444636897947817 1107 2 0.48857161593956633 1107 3 0.5921804361616516 1107 4 0.4386502083416133 1107 5 0.3283633753956394 1107 6 0.08499909015336726 1107 7 0.3764947689041307 1107 8 0.46038775569485324 1107 9 0.6269749721512738 1107 10 0.1133488442329309 1107 11 0.2892684750085124 1107 12 0.34212792274658455 1107 13 0.26622499950624956 1107 14 0.5818238652883494 1107 15 0.6540013748497202 1107 16 0.5852519495591508 1107 17 0.48669926469501235 1107 18 0.692926572442488 1107 19 0.8536575150923085 1107 20 0.5029201690238878 1107 21 0.8615999381178412 1107 22 0.6908552546526394 1107 23 0.7743278643891429 1107 24 0.53068003176773 1107 25 0.5880239700753478 1108 1 1.0177476848892795 1108 2 0.8849343903765039 1108 3 0.7786356785154709 1108 4 0.4094619015335468 1108 5 -0.6820075999585171 1108 6 0.12489210612949364 1108 7 0.47697019505856275 1108 8 0.5277483784684307 1108 9 0.8765195700256783 1108 10 0.800513073854404 1108 11 0.5041688751737539 1108 12 0.4801139784543835 1108 13 0.41883542860909984 1108 14 0.7446228986690926 1108 15 0.6833158172724936 1108 16 0.6372397942904158 1108 17 0.7036830047631426 1108 18 0.6871537658094411 1108 19 0.656713045245553 1108 20 0.6961178855488086 1108 21 0.6677442141362064 1108 22 0.7269741879237444 1108 23 1.0357265822206259 1108 24 0.797354897249356 1108 25 0.8557184317758484 1109 1 0.47574789324991684 1109 2 -0.4675513184361435 1109 3 0.44120458595517675 1109 4 0.5133470432915956 1109 5 -0.7693112676658629 1109 6 0.15326724041568696 1109 7 0.6592224615406153 1109 8 0.752839480236922 1109 9 1.0759005372895345 1109 10 -1.2900347465924895 1109 11 0.07978642362039778 1109 12 0.6503911093059784 1109 13 0.670080831512486 1109 14 1.235319484131118 1109 15 1.0531520301274497 1109 16 1.0948725709871852 1109 17 1.2003901225193943 1109 18 1.129980614758061 1109 19 1.0168151195541242 1109 20 1.2722837809450454 1109 21 1.231364782691898 1109 22 1.0802922190725397 1109 23 1.366645844713581 1109 24 1.0636162688672712 1109 25 1.3608518329944204 1110 1 0.8132640964241062 1110 2 -0.1291854698255328 1110 3 0.4763704665182651 1110 4 0.7179463846348355 1110 5 0.9524643460577238 1110 6 0.7669036865739471 1110 7 0.8941585021202627 1110 8 1.133084860743729 1110 9 1.116857804481242 1110 10 0.7268982512398239 1110 11 0.799998907598777 1110 12 0.93410914321192 1110 13 0.7963857089856248 1110 14 1.1897636017888544 1110 15 0.9284084669178228 1110 16 0.564806378524338 1110 17 0.7997096799775747 1110 18 0.9109899499649714 1110 19 1.168342484309818 1110 20 0.8766267762719977 1110 21 0.8487247818204651 1110 22 0.8904218807756834 1110 23 1.1872544667992742 1110 24 1.1241262739869313 1110 25 1.262935642891705 1111 1 0.13482979205012668 1111 2 0.3069922392060036 1111 3 0.39398497197499793 1111 4 0.10240285967135776 1111 5 0.6003392279001218 1111 6 0.07613630666503468 1111 7 0.43219064098767035 1111 8 0.32110239103504057 1111 9 0.6627132655107175 1111 10 0.4866267035900833 1111 11 0.2835840240377223 1111 12 0.22910785421991164 1111 13 0.3131168252527061 1111 14 0.6543283521540673 1111 15 0.4713679925687657 1111 16 0.3402038177268807 1111 17 0.32321745689602466 1111 18 0.4448371614455908 1111 19 0.22662210964280405 1111 20 0.2925759040540683 1111 21 0.45075967053748656 1111 22 0.4312468356609642 1111 23 0.6727386981303738 1111 24 0.4877268583299781 1111 25 0.6226984683621808 1112 1 0.24029434547584572 1112 2 -0.1899844985474317 1112 3 0.18895855646314885 1112 4 0.43992726981712593 1112 5 0.4704999337889859 1112 6 0.36971470956383 1112 7 0.5314211336162202 1112 8 0.5925255094484498 1112 9 0.05949140287138022 1112 10 0.6922184940362399 1112 11 0.0513731425064492 1112 12 0.3890364908475732 1112 13 -0.04408174747985072 1112 14 0.5151238601340467 1112 15 0.34003781250071796 1112 16 0.3770278710557875 1112 17 0.46995106129953257 1112 18 0.4098685557973738 1112 19 0.4448667919651612 1112 20 0.26921408069224384 1112 21 0.5759022589810456 1112 22 0.28907274222473234 1112 23 0.5427904227452061 1112 24 0.21062177714722452 1112 25 0.3828113220672691 1113 1 -0.21833007667936144 1113 2 -0.029951033180303455 1113 3 -1.3104606445275806 1113 4 -1.132257243469759 1113 5 -1.4514094828359276 1113 6 -0.6626635058056607 1113 7 -0.29567269703389343 1113 8 -0.5105677918060252 1113 9 0.31105831942539197 1113 10 0.09269093237583093 1113 11 -0.04380463043954563 1113 12 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0.2766659909666416 2202 18 0.3750174505994437 2202 19 0.2568565431431793 2202 20 0.304026857340169 2202 21 0.4038361062266773 2202 22 0.14058155140314454 2202 23 0.49756215300048723 2202 24 0.2852811466774199 2202 25 0.2897867697273142 2203 1 0.24660422681743555 2203 2 0.299268125543648 2203 3 0.053659468324081416 2203 4 -0.08407730220073262 2203 5 0.18931433126952868 2203 6 -0.3237176565618469 2203 7 0.22709870819977596 2203 8 0.4688669727188639 2203 9 0.5074462645429989 2203 10 0.527942794027318 2203 11 0.144525345406592 2203 12 0.2237502400736986 2203 13 0.19002366082937527 2203 14 0.4316011594031224 2203 15 0.35188000101426553 2203 16 0.32003138876585524 2203 17 0.3714746927542052 2203 18 0.4697707411060108 2203 19 0.6019894009245874 2203 20 0.47227151415348345 2203 21 0.35785213116913894 2203 22 0.37443501196077733 2203 23 0.379276922815869 2203 24 0.35558821136947927 2203 25 0.44763380750179493 2204 1 0.11761083863777344 2204 2 0.29902202801246247 2204 3 0.45894503564572986 2204 4 0.012639635203605337 2204 5 0.13866502875854536 2204 6 -0.1319311160915646 2204 7 0.03652138769754032 2204 8 0.15557327020277256 2204 9 0.4718557003477031 2204 10 0.34119365734353124 2204 11 0.21132229185273133 2204 12 0.4841688246300748 2204 13 0.23737707936714258 2204 14 0.5751714208559235 2204 15 0.5188805703806523 2204 16 0.3118390753943743 2204 17 0.3542916162434011 2204 18 0.520419435968769 2204 19 0.5920579983327201 2204 20 0.44012145420898463 2204 21 0.609526050117374 2204 22 0.7350602576785263 2204 23 0.8553219106598675 2204 24 0.4059537421593191 2204 25 0.43042764035843106 2205 1 0.8055654592241481 2205 2 0.8359623051623721 2205 3 0.7731123184996065 2205 4 0.5598559291792213 2205 5 0.4377009732070718 2205 6 0.4762783665331227 2205 7 0.6000546693996469 2205 8 0.5645422692694382 2205 9 0.9169192523194454 2205 10 0.7904420257615712 2205 11 0.9028940189641546 2205 12 0.7893818268226482 2205 13 0.7884592910061272 2205 14 1.1529990776741101 2205 15 0.8920377033754576 2205 16 0.8826188354843651 2205 17 0.9328166451253495 2205 18 0.8347204694110886 2205 19 0.7828879645387943 2205 20 0.9033318654949625 2205 21 1.1633218217297738 2205 22 1.0060109148086056 2205 23 1.1429873028995274 2205 24 0.8313290906993345 2205 25 1.15213530245655 2206 1 0.2062117710440461 2206 2 0.4707291104055642 2206 3 0.5573293611402832 2206 4 0.5933426053872211 2206 5 0.6935177795861378 2206 6 0.5338035261233003 2206 7 0.6664305525410157 2206 8 0.6909365535557725 2206 9 0.89771050155273 2206 10 0.9434702635679741 2206 11 0.5446896072328542 2206 12 0.6195721991618655 2206 13 0.5642776876075928 2206 14 0.7292979486162068 2206 15 0.69378921976611 2206 16 0.7486891687495429 2206 17 0.669198661981703 2206 18 0.62338331954399 2206 19 0.4866829167185358 2206 20 0.6029683860883424 2206 21 0.659488249863081 2206 22 0.6885934359926367 2206 23 0.801367774968405 2206 24 0.7507229887887945 2206 25 0.8289339575827483 2207 1 -1.127673400797896 2207 2 -0.10577915287572992 2207 3 0.26560693876938046 2207 4 0.37892904102512404 2207 5 0.3159458690240058 2207 6 0.3222921176484306 2207 7 0.5536210369008217 2207 8 0.5752020078932816 2207 9 0.9068001109768744 2207 10 0.7006241493116647 2207 11 0.5414128873838209 2207 12 0.201904789085809 2207 13 0.4725037984327827 2207 14 -5.076271903273771 2207 15 -1.5556818158603534 2207 16 -0.543959656091765 2207 17 -0.48768931194177867 2207 18 0.166395271279562 2207 19 0.14743947873603958 2207 20 0.46013656816645465 2207 21 0.5205936424404243 2207 22 0.3946951115230348 2207 23 0.6287795665850382 2207 24 0.5454790933235927 2207 25 0.5971045986303807 2208 1 0.4572609275521273 2208 2 0.8527225077709204 2208 3 0.8258797730036598 2208 4 0.6986530253703407 2208 5 0.7038191716950308 2208 6 0.7175907460722328 2208 7 1.0353094935553386 2208 8 1.003768421417604 2208 9 1.2866842085367765 2208 10 1.241457955744174 2208 11 0.9758940004421817 2208 12 0.9733136921265384 2208 13 0.8426006397758883 2208 14 1.298667279485123 2208 15 1.041941443614654 2208 16 1.2571395795445355 2208 17 1.0687510076264246 2208 18 0.9784923484866042 2208 19 0.6681661590608741 2208 20 0.8278286944249729 2208 21 1.0800563114876738 2208 22 0.9710686761127221 2208 23 1.3113171334017224 2208 24 1.120905362532099 2208 25 0.9358902284830748 2209 1 -0.8113077740874388 2209 2 -0.372150959292032 2209 3 0.06466938911102815 2209 4 0.23613899211716927 2209 5 0.28292328661321875 2209 6 0.24949694841277648 2209 7 0.3994956418410567 2209 8 0.4072570249316101 2209 9 0.47431251452931633 2209 10 0.2812640137112652 2209 11 0.32892281905393617 2209 12 0.407500071321806 2209 13 0.47937386570329465 2209 14 0.6935022726183107 2209 15 0.4928836095682527 2209 16 0.4876521877558986 2209 17 0.5170355145850465 2209 18 0.5549822373971196 2209 19 0.6067381704040287 2209 20 0.49076672159972645 2209 21 0.5158458627204539 2209 22 0.6176165233209758 2209 23 0.8144761959969031 2209 24 0.9024665750051781 2209 25 0.7447465994425713 2210 1 -0.3817885141541666 2210 2 -0.6100223868850527 2210 3 0.12351198306252087 2210 4 0.46950241145243865 2210 5 0.6631394166238778 2210 6 0.6396524661366101 2210 7 0.7561444036675482 2210 8 0.7196940400088536 2210 9 1.0326377760084782 2210 10 0.9468189659161013 2210 11 0.8366779141061329 2210 12 0.35624868932252696 2210 13 0.4847905690602019 2210 14 0.9056954198587164 2210 15 0.5388980704173107 2210 16 0.8092177361038537 2210 17 0.7372136824209079 2210 18 0.6876456068336552 2210 19 0.773059948755801 2210 20 0.78660751528313 2210 21 0.8335185759397064 2210 22 0.8641590791146072 2210 23 0.9321430387767577 2210 24 0.7018186252561378 2210 25 0.9188308108957983 2211 1 -1.0905116431350592 2211 2 -0.09546725153473545 2211 3 0.3550555738268528 2211 4 0.6852269337454331 2211 5 0.9394133019741986 2211 6 0.6002913992576282 2211 7 0.7815424928395203 2211 8 0.8234808632572665 2211 9 1.148864885881089 2211 10 1.1281834165530498 2211 11 0.954029625889568 2211 12 0.964698131997686 2211 13 0.8364675196971447 2211 14 1.217777426228146 2211 15 0.7512985481243177 2211 16 1.0133230306898469 2211 17 0.8601734754145411 2211 18 1.032933226260404 2211 19 1.0823802114946588 2211 20 1.0804520614614788 2211 21 1.2451925409078584 2211 22 1.0311773912165165 2211 23 1.348161151834077 2211 24 1.203276626848492 2211 25 1.328549162806631 2212 1 1.0399416365679102 2212 2 0.22318219153457264 2212 3 0.6259688370281715 2212 4 0.7441997060226555 2212 5 0.5883406947134731 2212 6 0.6950985362213231 2212 7 0.7130193905292899 2212 8 0.8098592667226114 2212 9 0.9966809956170621 2212 10 0.7301507226586565 2212 11 0.451871519453924 2212 12 0.45292523595808376 2212 13 0.42901341491167244 2212 14 0.6379403800235224 2212 15 0.435405396309136 2212 16 0.02445390940271608 2212 17 0.4279075925762541 2212 18 0.6549655076135394 2212 19 0.44629574367287717 2212 20 0.373745687699099 2212 21 0.6331102389276637 2212 22 0.7977924902264206 2212 23 1.1684556031542144 2212 24 1.087334485759385 2212 25 0.5856577642917742 2213 1 0.2488076408666835 2213 2 0.12091584022638102 2213 3 -0.5734133941330448 2213 4 -1.1448133888352139 2213 5 -2.226999078147859 2213 6 -0.5463887990858751 2213 7 -0.7032983964368498 2213 8 -0.5044173222266082 2213 9 -0.9719032865251904 2213 10 -1.0175071076405047 2213 11 -0.9315911511374537 2213 12 -1.160420383695424 2213 13 -1.2269199857518487 2213 14 -8.646785019281882 2213 15 -3.8074443681790804 2213 16 -2.620372888011115 2213 17 -1.865203867654337 2213 18 -5.606764763059559 2213 19 -3.5250133968314117 2213 20 -5.5026322072830745 2213 21 -2.6873965501993773 2213 22 -2.0349761615297766 2213 23 -4.87898915875877 2213 24 -2.6370153031863772 2213 25 -1.9218370348829978 2214 1 0.2650024405206466 2214 2 0.05600004778828094 2214 3 -1.7434861935812727 2214 4 -2.127623646525361 2214 5 -0.4302675490896277 2214 6 -0.49111636186422913 2214 7 -0.33738884178330153 2214 8 -0.27845488384138406 2214 9 -5.078947311894542 2214 10 -1.461164685477904 2214 11 -0.8533068707255183 2214 12 -1.1080079850235904 2214 13 -1.0919551713086548 2214 14 -2.3049438020116995 2214 15 -2.1504845756667805 2214 16 -1.4749763527724369 2214 17 -1.7471615100786906 2214 18 -1.197949571128376 2214 19 -4.489611144888322 2214 20 -2.1151037291574752 2214 21 -3.7699337888793742 2214 22 -6.143333005301859 2214 23 -6.326226843813888 2214 24 -5.5228068475228795 2214 25 -3.470497319301689 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24890.0 3 13.0 4 518.0 5 3053.0 6 3577.0 7 2994.0 8 2240.0 9 1841.0 10 1590.0 11 1486.0 12 1376.0 13 1443.0 14 1450.0 15 1449.0 16 1518.0 17 1590.0 18 1831.0 19 2102.0 20 2713.0 21 3488.0 22 4486.0 23 5518.0 24 6712.0 25 7998.0 26 9014.0 27 10187.0 28 11711.0 29 14313.0 30 17557.0 31 21920.0 32 28250.0 33 36142.0 34 47246.0 35 62411.0 36 85249.0 37 124980.0 38 205244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.47637644939654 18.93162719532717 8.687293766311061 30.904702588965232 2 13.667111493644764 21.175343424851878 38.92354503412533 26.23400004737802 3 18.27624283215564 24.566968098897497 28.89630003013884 28.26048903880803 4 12.298214628830731 17.138957835971162 37.13179845283624 33.43102908236186 5 13.118745106296181 38.65154196906192 35.04280140280693 13.186911521834965 6 32.56945434951425 37.684696535074515 17.134450096937616 12.611399018473616 7 28.699936452353768 31.299852601102724 21.607122610041582 18.393088336501926 8 25.204340092587586 38.24676925754565 19.47714779600141 17.071742853865356 9 26.392817254833705 15.393876100546827 19.285496714677343 38.92780992994213 10 14.832140493268644 29.583223128859625 34.02074607441796 21.563890303453775 11 34.713982925414136 22.27616412547676 24.43055111521596 18.579301833893144 12 24.089650227283194 24.463434048403755 31.20338392111201 20.243531803201044 13 29.585975529535585 22.10590711748454 25.167477963425867 23.140639389554003 14 20.69575223289518 22.59186994438827 26.979061341422312 29.733316481294235 15 22.551806978466768 32.188674628098845 22.50062822251839 22.758890170915993 16 21.9512708547401 28.13996966104839 28.152994622927352 21.755764861284156 17 21.38556753115527 29.327793448972717 27.70411439036713 21.582524629504885 18 21.564770752283007 27.67102633562829 29.833450208072122 20.93075270401659 19 23.755075083608958 26.94392476640801 26.65211568164655 22.648884468336476 20 23.883556201091448 27.66967682859443 27.46601727159344 20.98074969872068 21 22.481018919903857 26.807297470461084 26.669815379312432 24.04186823032263 22 23.407051703723194 28.690698592290488 26.180897250361795 21.721352453624522 23 21.92487830548612 28.448493619260624 27.319957900276282 22.30667017497698 24 23.590773821270414 26.873771539985636 27.938502666761387 21.596951971982563 25 22.466912248388372 27.86409682936456 27.627154321799765 22.041836600447308 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 127.0 1 127.0 2 131.5 3 136.0 4 136.0 5 136.0 6 233.5 7 331.0 8 331.0 9 331.0 10 554.5 11 778.0 12 778.0 13 778.0 14 1455.0 15 2132.0 16 2132.0 17 2132.0 18 3990.5 19 5849.0 20 5849.0 21 5849.0 22 10254.5 23 14660.0 24 14660.0 25 14660.0 26 22688.0 27 30716.0 28 30716.0 29 30716.0 30 42538.5 31 54361.0 32 54361.0 33 54361.0 34 63751.0 35 73141.0 36 73141.0 37 73141.0 38 80308.0 39 87475.0 40 87475.0 41 87475.0 42 92674.5 43 97874.0 44 97874.0 45 97874.0 46 101669.5 47 105465.0 48 105465.0 49 105465.0 50 101353.5 51 97242.0 52 97242.0 53 97242.0 54 85366.0 55 73490.0 56 73490.0 57 73490.0 58 62998.5 59 52507.0 60 52507.0 61 52507.0 62 42860.0 63 33213.0 64 33213.0 65 33213.0 66 25511.0 67 17809.0 68 17809.0 69 17809.0 70 12890.0 71 7971.0 72 7971.0 73 7971.0 74 5577.0 75 3183.0 76 3183.0 77 3183.0 78 2173.0 79 1163.0 80 1163.0 81 1163.0 82 763.5 83 364.0 84 364.0 85 364.0 86 228.0 87 92.0 88 92.0 89 92.0 90 55.5 91 19.0 92 19.0 93 19.0 94 10.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08498881726088672 2 0.03341665570319695 3 0.037231943165372976 4 0.033942902249703984 5 0.025654519142218127 6 0.38310748585712406 7 0.21234048151559004 8 0.22141823444283645 9 0.22720694645441392 10 0.019602683857387185 11 0.003025917642415472 12 0.004604657281936588 13 0.0 14 0.07209577687146428 15 0.0024996710959084333 16 0.002762794369161952 17 0.004736218918563347 18 0.0035521641889225105 19 0.001841862912774635 20 0.0010524930930140772 21 1.3156163662675965E-4 22 0.0 23 0.0 24 2.631232732535193E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 760100.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.51286102307088 #Duplication Level Percentage of deduplicated Percentage of total 1 91.69619390204168 75.66115303783725 2 6.235299757455637 10.289848446482493 3 1.069061481402341 2.6463396432020896 4 0.3557846614878495 1.17427241309949 5 0.1836287562091996 0.7575867020464525 6 0.09616187171195535 0.4760754693772166 7 0.06611563579761334 0.38187731876143083 8 0.04662281755555586 0.3077585652372456 9 0.03559766923524995 0.26435389809180826 >10 0.1834670378538617 2.736352818167272 >50 0.015071663843167984 0.8914170700060012 >100 0.014910484195617243 2.488394527694392 >500 0.0011222944978537637 0.6646301988001492 >1k 9.619667124460832E-4 1.2599398911967796 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1868 0.245757137218787 No Hit GTCCTACAGTGGACATTTCTAAATT 1839 0.24194184975661098 No Hit CTTTAGGACGTGAAATATGGCGAGG 1581 0.207998947506907 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1576 0.20734113932377318 No Hit GTCCTACAGTGTGCATTTCTCATTT 1441 0.18958031837916064 No Hit CTGTAGGACCTGGAATATGGCGAGA 1219 0.16037363504802 No Hit CTGAAGGACCTGGAATATGGCGAGA 899 0.11827391132745692 No Hit CCCATGTACTCTGCGTTGATACCAC 874 0.11498487041178793 No Hit GTATCAACGCAGAGTACTTTTTTTT 839 0.11038021312985134 No Hit GTACATGGGAAGCAGTGGTATCAAC 762 0.10024996710959085 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.3156163662675965E-4 13 0.0 0.0 0.0 1.3156163662675965E-4 1.3156163662675965E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGACCG 35 0.0021093006 16.353683 5 TAGGGTG 70 6.945389E-6 13.628069 5 ACTGATC 50 0.001498311 13.300001 8 TAGGATC 60 3.9465606E-4 12.719531 4 TAGGACA 345 0.0 12.44302 4 GAACAGT 125 1.3169483E-9 12.209941 6 GTATTGG 55 0.0029776709 12.138155 1 GGCATGA 55 0.0029776709 12.138155 1 TGTAGGA 1215 0.0 12.097016 2 CTGTAGG 1205 0.0 11.871936 1 GGCGAGG 465 0.0 11.8151655 19 CTAGGAC 105 2.5609916E-7 11.814124 3 ATAGGAC 130 2.4283509E-9 11.744218 3 GTAGGCA 65 7.739805E-4 11.741106 4 TGGCGAG 1175 0.0 11.690241 18 GTCCTAC 965 0.0 11.661973 1 TAGGACC 435 0.0 11.623021 4 GTAGGAC 1210 0.0 11.513701 3 GGATTAT 75 1.9974392E-4 11.444546 1 CTAAATT 555 0.0 11.435274 19 >>END_MODULE