FastQCFastQC Report
Thu 26 May 2016
SRR1926446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712672
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14280.200372681963091No Hit
GTCCTAAAGTGTGTATTTCTCATTT11790.16543374792330834No Hit
CTGTAGGACGTGGAATATGGCAAGA10930.1533664855641866No Hit
CTTTAGGACGTGAAATATGGCGAGG8820.12375959768308561No Hit
GTCCTACAGTGTGCATTTCTCATTT8790.12333864667055812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGA404.7556584E-420.4128422
GTATTGA450.001237354817.7074451
CTCTAGG908.8493834E-917.7074451
TAGGACC3500.016.7983464
TGTAGGA8300.016.3958572
GTATTAA604.710735E-415.4940161
CTGTAGG8550.015.222191
GTCCTAC8350.014.9505991
GTATAAA801.6368263E-514.9406581
GGTCTGG550.004721810614.4879111
GTCTTAC658.6445716E-414.3021691
GTTTAAA852.9007966E-514.0617961
GTAGGAC9550.014.0362353
AGGACCT5900.013.7412925
ACCTACA1007.0290025E-613.6085622
ACGAAAT400.00722561813.58868812
TCCTACA9800.013.3308362
GTTCTAG600.0084027613.2805851
GTTTAAT600.0084027613.2805851
CCACTAT600.0084027613.2805851
Produced by FastQC (version 0.11.5)